| Project/Area Number |
25860144
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SAITO Yuki 札幌医科大学, 医学部, 研究員 (40758702)
OZASA Yasuhiro 札幌医科大学, 医学部, 助教 (40404636)
FUJIMIYA Mineko 札幌医科大学, 医学部, 教授 (10199359)
IBA Kosuke 札幌医科大学, 医学部, 准教授 (60363686)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 手根管症候群 / 間葉系幹細胞 / 手根管症候群病態 |
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| Outline of Final Research Achievements |
An increased risk of carpal tunnel syndrome (CTS) has been reported in postmenopausal women. Fibrosis of subsynovial connective tissue (SSCT) is a prominent feature of CTS. Mesenchymal stem cells (MSCs) is considered to maintain the turnover of skeletal tissues in homeostasis. The purpose of this study was to assess the effect of the estradiol (E2) on SSCT-derived MSC (SSCT-MSC) of CTS patients in vitro. The proliferation activity of the SSCT-MSC was increased with E2. P16 mRNA expression and TP53 expression was significantly downregulated with E2. TGF-β was also downregulated with E2. We demonstrated that the effect of estradiol on SSCT-MSC in CTS patients, which increased the cell proliferation and inhibited the cellular senescence with downregulation of the TGF-β. Our findings suggested that the functional deterioration of SSCT-MSC might reside in the postmenopausal CTS patients, and relate to the homeostatic imbalance in SSCT fibrosis.
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