| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Outline of Final Research Achievements |
Atg9 is a membrane protein essential for autophagy and considered to be directly involved in the autophagosome formation. Mammalian Atg9A was reported to be localized to trans-Golgi network and endosomal compartment. For dissecting the Atg9A-containing membranes, we examined subcellular localization of Atg9A and performed immunoisolation of those membranes. Atg9A-GFP in human culture cells was observed as numerous puncta that move rapidly throughout the cytoplasm. We succeeded in isolation of these cytoplasmic membranes, and revealed that they were small vesicles by electron microscopy. Proteomic analyses revealed that isolated these vesicles contained proteins including Rab1, a small GTPase in ER-Golgi vesicle trafficking. In Rab1B-depleted cells, Atg9A accumulated on intermediate membrane structures at autophagosome formation site. These results indicated that Rab1B is involved in the regulation of proper development of autophagosome.
|
