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The regulation of in vitro megakaryocyte differentiation and platelet production by hypoxia

Research Project

Project/Area Number 25860150
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field General anatomy (including histology/embryology)
Research InstitutionWaseda University

Principal Investigator

KANAI MAI  早稲田大学, ナノ理工学研究機構, 招聘研究員 (30528526)

Research Collaborator GODA Nobuhito  早稲田大学, 理工学術院, 教授 (00245549)
HOUKUWA Kaori  早稲田大学, 理工学術院
MATHUBARA Yumiko  慶應義塾大学, 医学部, 講師 (26461410)
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords細胞分化 / 巨核球 / 糖代謝 / 脂質代謝 / ミトコンドリア代謝 / 低酸素応答 / ミトコンドリア
Outline of Final Research Achievements

The skill of producing platelets in vitro is needed for over tens of thousands of patients with various kinds of platelet disorders. In this study, we aimed to investigate relevance of metabolic systems to megakaryocyte(MK) differentiation and their regulatory mechanism in MK differentiation using preadipocytes isolated from subcutaneous adipose tissues of mice. In MK differentiation, we found characteristic metabolic alterations such as activation of glycolysis, increased cholesterol levels, and enhanced mitochondrial biogenesis. Moreover, hypoxia-inducible factor α (HIFα), a central transcription factor that controls adaptive response to hypoxic stress in normal and pathological conditions, do not affect MK differentiation unlike the regulation of the hematopoietic stem cells.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (5 results)

All 2015 2014 Other

All Presentation (5 results)

  • [Presentation] The regulation of in vitro megakaryocyte differentiation by hypoxia.2015

    • Author(s)
      Kanai M, Houkuwa K, Goda N
    • Organizer
      私立大学戦略的研究基盤形成支援事業 「ストレス応答制御に基づく次世代健康寿命科学の研究拠点形成」第三回成果報告会
    • Place of Presentation
      東京(早稲田大学)
    • Year and Date
      2015-03-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] 低酸素応答による巨核球分化機構の解明2014

    • Author(s)
      金井 麻衣, 法桑 かおり, 合田 亘人
    • Organizer
      第37回分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] マウス脂肪前駆細胞由来巨核球における脂質代謝を介した分化制御機構の解明2014

    • Author(s)
      法桑 かおり, 金井 麻衣, 合田 亘人
    • Organizer
      第37回分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Relevance of metabolic systems to in vitro megakaryocyte differentiation.

    • Author(s)
      Kanai M, Houkuwa K, Matsubara Y, Ikeda Y, Goda N.
    • Organizer
      第36回分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド
    • Related Report
      2013 Research-status Report
  • [Presentation] in vitroにおけるにおける巨核球分化誘導と代謝制御との関連性

    • Author(s)
      金井 麻衣, 法桑 かおり, 松原 由美子, 池田 康夫, 合田 亘人
    • Organizer
      私立大学戦略的研究基盤形成支援事業 「ストレス応答制御に基づく次世代健康寿命科学の研究拠点形成」第二回成果報告会
    • Place of Presentation
      早稲田大学
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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