Morphological analysis of circuitry of cholinergic C-terminal inputs to oro-facial motor neurons

• Project/Area Number: 25860152
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including histology/embryology)
• Research Institution: National Defense Medical College
• Principal Investigator: MATSUI Toshiyasu 防衛医科大学校(医学教育部医学科進学課程及び専門課程、動物実験施設、共同利用研究, 医学教育部医学科専門課程, 助教 (90531343)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 運動ニューロン / 脳幹網様体 / コリン作動性ニューロン / 脳神経運動核 / 神経トレーサー / 介在ニューロン / ウイルスベクター / partition細胞

Outline of Final Research Achievements

Large cholinergic synaptic boutons, named C-terminals, contact motor neurons innervating oro-facial muscles and regulate their excitability. In the present study, we first determined the distribution of cholinergic interneurons that gave rise to C-terminals in oro-facial motor nuclei, using neuronal tracing techniques. Next, we produced viral vectors for the tracer to visualize cholinergic neurons in a single-cell level, and examined morphological characteristics of cholinergic interneurons that are origins of C-terminals in the spinal cord.

Research Products

• [Journal Article] VALUE OF A NOVEL PGA-COLLAGEN TUBE ON RECCURENT LARYNGEAL NERVE REGENERATION IN A RAT MODEL (2015)
  • Author(s): Suzuki H, Araki K, Matsui T, Tomifuji M, Yamashita T, Kobayashi Y, Shiotani A
  • Journal Title: Laryngoscope Volume: Epub head of print Issue: 7 Pages: 0-0
  • DOI: 10.1002/lary.25750
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Neuroprotection by JM-1232(-) against oxygen-glucose deprivation-induced injury in rat hippocampal slice culture (2015)
  • Author(s): Takahiro Ogura, Tsuyoshi Hamada, Toshiyasu Matsui, Shinji Tanaka, Shigeo Okabe, Tomiei Kazama, Yasushi Kobayashi
  • Journal Title: Brain Research Volume: 1594 Pages: 52-60
  • DOI: 10.1016/j.brainres.2014.10.038
  • Peer Reviewed
• [Journal Article] C-terminals in the mouse branchiomotor nuclei originate from the magnocellular reticular formation (2013)
  • Author(s): Toshiyasu Matsui, Yu Hongo, Yoshinori Haizuka, Kenichi Kaida, George Matsumura, Donna M. Martin, Yasushi Kobayashi
  • Journal Title: Neuroscience Letters Volume: 548 Pages: 137-142
  • DOI: 10.1016/j.neulet.2013.05.063
  • Peer Reviewed
• [Journal Article] Organic cation transporter 2 (SLC22A2), a low-affinity and high-capacity choline transporter, is preferentially enriched on synaptic vesicles in cholinergic neurons. (2013)
  • Author(s): Nakata T, Anzai N, et al.
  • Journal Title: Neuroscience Volume: 252 Pages: 212-221
  • DOI: 10.1016/j.neuroscience.2013.08.011
  • Peer Reviewed
• [Presentation] マウス脳において有機カチオントランスポーターOCT2はモノアミン作動性・コリン作動性神経の軸索終末に分布する (2016)
  • Author(s): 松井利康，中田隆博，小林 靖
  • Organizer: 第121回日本解剖学会総会・学術集会
  • Place of Presentation: 郡山
  • Year and Date: 2016-03-28
• [Presentation] Changes of neuronal activities in the auditory pathway on a rat tinnitus model induced by laser-induced shock waves (2016)
  • Author(s): Katsuki Niwa, Kunio Mizutari, Toshiyasu Matsui, Takaomi Kurioka, Takeshi Matsunobu, Satoko Kawauchi, Yasushi Sato, Shunichi Sato, Akihiro Shiotani, Yasushi Kobayashi
  • Organizer: ARO 39th MidWinter Meeting
  • Place of Presentation: San Diego
  • Year and Date: 2016-02-20
  • Int'l Joint Research
• [Presentation] レーザー誘起衝撃波を利用した難聴耳鳴モデル (2015)
  • Author(s): Katsuki Niwa, Kunio Mizutari, Toshiyasu Matsui, Takaomi Kurioka, Takeshi Matsunobu, Atsushi Tamura, Satoko Kawauchi, Yasushi Sato, Shunichi Sato, Akihiro Shiotani, Yasushi Kobayashi
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸
  • Year and Date: 2015-07-28
• [Presentation] Morphological analysis of spinal cholinergic interneuron, partition cells (2014)
  • Author(s): 本郷 悠，松井利康，中田隆博，古川敬世，小野岳史，海田賢一，宮平 靖，小林 靖
  • Organizer: The 37th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] OCT2, a low-affinity and high-capacity choline transporter, is preferentially enriched on synaptic vesicles in cholinergic neurons (2014)
  • Author(s): 中田隆博，松井利康，小林久美子，小林 靖，安西尚彦
  • Organizer: The 37th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] マウス脳における有機カチオントランスポーターOCT2の局在解析 (2014)
  • Author(s): 松井利康，中田隆博，安西尚彦，小林 靖
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 札幌
  • Year and Date: 2014-09-09 – 2014-09-12