Ubiquitin-independent endosomal sorting of cytokine receptors

• Project/Area Number: 25860161
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General physiology
• Research Institution: Shinshu University
• Principal Investigator: AMANO Yuji 信州大学, 学術研究院医学系, 助教 (50624681)
• Research Collaborator: TAKESHITA Toshikazu 信州大学, 学術研究院医学系, 教授 (60212023) ; KOJIMA Katsuhiko 信州大学, 学術研究院医学系, 講師 (80345743) ; YOSHINO Kazuhisa 信州大学, 学術研究院医学系, 助教 (40551859)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 小胞輸送 / インターロイキン２受容体 / インターロイキン４受容体 / ユビキチン非依存性小胞輸送 / ユビキチン

Outline of Final Research Achievements

We had reported that ubiquitin-independent endosomal sorting mechanism of interleukin-2 receptor β (IL-2Rβ) and interleukin-4 receptor α (IL-4Rα）. Hrs, which is also involved in an ubiquitin-dependent endosomal sorting, binds the hydrophobic amino acids clusters of the receptors and sorts the receptors into endosomes. Here, we elucidated that the hydrophobic amino acids clusters act as endosomal sorting signal and suggest Hrs controls endosomal sorting by novel manner.

Research Products

• [Journal Article] ESCRT-0 protein hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) is targeted to endosomes independently of signal-transducing adaptor molecule (STAM) and the complex formation with STAM promotes its endosomal dissociation (2014)
  • Author(s): Kojima, K., Amano, Y., Yoshino, K., Tanaka, N., Sugamura, K., Takeshita, T.
  • Journal Title: J Biol Chem Volume: 289 Issue: 48 Pages: 33296-33310
  • DOI: 10.1074/jbc.m114.578245
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A hydrophobic amino acid cluster inserted into the C-terminus of a recycling cell surface receptor functions as an endosomal sorting signal (2013)
  • Author(s): Amano Y., Yoshino K., Kojima K., Takeshita T.
  • Journal Title: Biochem Biophys Res Commun Volume: 441 Issue: 1 Pages: 164-168
  • DOI: 10.1016/j.bbrc.2013.10.019
  • Peer Reviewed
• [Presentation] Hydrophobic amino acid cluster in the cytoplasmic tail of interleukin-2 receptor β chain acts as a sorting signal for lysosome
  • Author(s): 天野勇治
  • Organizer: 国際免疫学会
  • Place of Presentation: ミラノ(イタリア）