Molecular mechanism of the substrate selectivity in Voltage-sensing phosphatase
| Project/Area Number |
25860163
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General physiology
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| Research Institution | Osaka Medical College (2015)
Osaka University (2013-2014) |
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Principal Investigator |
SAKATA Souhei 大阪医科大学, 医学部, 講師 (40528006)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 膜電位 / イノシトールリン脂質 / 非天然アミノ酸 / 電位依存性ホスファターゼ / 脱リン酸化酵素 |
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| Outline of Final Research Achievements |
Voltage-sensing phosphatase, VSP, has an activity to dephosphorylate several kinds of phosphoinositides in a voltage-dependent manner. Our recent study has shown that VSP mainly dephosphoryltaes PI(3,4,5)P3 in low membrane voltages but dephosphorylates PI(3,4)P2 in high voltages. However, little is know about the molecular mechanisms how the membrane voltage regulates the substrate specificity of VSP. In this work, I introduced a fluorescent label using the method of the genetic incorporation of a fluorescent unnatural amino acid to reveal the conformational change of the catalytic region which is responsible for the dephosphorylation activity. Results showed that Cα2 loop located in the catalytic region plays key roles in the substrate specificity in VSP.
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] A Kir3.4 mutation causes Andersen–Tawil syndrome by an inhibitory effect on Kir2.1.2014
Author(s)
Kokunai Y, Nakata T, Furuta M, Sakata S, Kimura H, Aiba T, Yoshinaga M, Osaki Y, Nakamori M, Itoh H, Sato T, Kubota T, Kadota K, Shindo K, Mochizuki H, Shimizu W, Horie M, Okamura Y, Ohno K, Takahashi MP
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Journal Title
Neurology
Volume: 82
Issue: 12
Pages: 1058-64
DOI
Related Report
Peer Reviewed / Open Access
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