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Protein 4.1R regulates degranulation of mast cells.

Research Project

Project/Area Number 25860168
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field General physiology
Research InstitutionTokyo Women's Medical University

Principal Investigator

TANAKA Shotaro  東京女子医科大学, 医学部, 助教 (90380667)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsマスト細胞 / 分泌顆粒 / ライブイメージング / 共焦点顕微鏡 / GFP / ライブセルイメージング / イメージング
Outline of Final Research Achievements

Mast cells cause various allergic symptoms by releasing inflammatory mediators such as histamine from their intracellular secretory granules (SGs) in response to allergic stimuli (degranulation). It is hoped that the development of treatments that inhibit degranulation will enable the mitigation of allergy symptoms. To do so, it is necessary to observe the intracellular dynamics of these SGs to elucidate the process of their degranulation.
In this study, we observed individual SGs by overexpressing green fluorescent protein (GFP) in the cytoplasm of mast cells and using confocal microscopy to photograph the “absence of GFP” representing SGs that arose as a result of GFP not penetrating the granular lumen. This imaging method has notable advantages that provide (i) whole SG images in the cell, (ii) accurate and clear outline of SGs, and (iii) the images of cell body and nucleus simultaneously. We regard that this method will contribute to the development of mast cell SG study.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (6 results)

All 2015 2014 2013

All Presentation (6 results)

  • [Presentation] マスト細胞分泌顆粒の分類の試み~構造および結合タンパク質に基づく~2015

    • Author(s)
      田中正太郎、高桑雄一
    • Organizer
      日本膜学会
    • Place of Presentation
      早稲田大学(東京都)
    • Year and Date
      2015-05-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] Observation of individual secretory granules in living mast cells using confocal microscopy2014

    • Author(s)
      Shotaro Tanaka, Yuichi Takakuwa
    • Organizer
      The 2014 ASCB/IFCB meeting
    • Place of Presentation
      Philadelphia, US
    • Year and Date
      2014-12-08
    • Related Report
      2014 Annual Research Report
  • [Presentation] 共焦点顕微鏡を用いたマスト細胞分泌顆粒の構造観察法の開発2014

    • Author(s)
      田中正太郎、高桑雄一
    • Organizer
      日本生化学会
    • Place of Presentation
      国際会議場(京都府)
    • Year and Date
      2014-10-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] マスト細胞分泌顆粒の新規イメージング方法の開発2014

    • Author(s)
      田中正太郎、高桑雄一
    • Organizer
      日本膜学会
    • Place of Presentation
      早稲田大学(東京都)
    • Year and Date
      2014-05-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] マスト細胞分泌顆粒の新規イメージング方法の開発2014

    • Author(s)
      田中正太郎、高桑雄一
    • Organizer
      日本膜学会
    • Place of Presentation
      東京都新宿区
    • Related Report
      2013 Research-status Report
  • [Presentation] Protein4.1Rによるマスト細胞の脱顆粒制御機構の解明2013

    • Author(s)
      田中正太郎、高桑雄一
    • Organizer
      日本生化学会
    • Place of Presentation
      神奈川県横浜市
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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