The dynamics of spatiotemporal dopamine release

• Project/Area Number: 25860169
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General physiology
• Research Institution: Tokyo University of Science
• Principal Investigator: SHINODA Yo 東京理科大学, 理工学部, 助教 (80403096)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2013: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
• Keywords: ドーパミン / CAPS2

Outline of Final Research Achievements

I established the primary culture method of DAergic neuron from mouse mesencephalon. The subcellular distributions of CAPS2 in the DAergic neuron from VTA and SNc are clarified by using anti-CAPS2 and anti-calbindin antibodies. Primary cultured DAergic neurons from WT and CAPS2 KO mice shows that the release activity of DA is significantly reduced in the DAergic neuron from CAPS2 KO mice.

Research Products

• [Journal Article] Lack of stress responses to long-term effects of corticosterone in Caps2 knockout mice. (2015)
  • Author(s): Yuriko Mishima, Yo Shinoda, Tetsushi Sadakata, Masami Kojima, Shigeharu Wakana, Teiichi Furuichi.
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 1-6
  • DOI: 10.1038/srep08932
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Axonal localization of Ca2+-dependent activator protein for secretion 2 is critical for subcellular locality of brain-derived neurotrophic factor and neurotrophin-3 release affecting proper development of postnatal mouse cerebellum. (2014)
  • Author(s): Sadakata T, Kakegawa W, Shinoda Y, Hosono M, Katoh-Semba R, Sekine Y, Sato Y, Saruta C, Ishizaki Y, Yuzaki M, Kojima M, Furuichi T.
  • Journal Title: PLoS One. Volume: 9(6) Issue: 6 Pages: 1-10
  • DOI: 10.1371/journal.pone.0099524
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Age-dependent redistribution and hypersialylation of the central myelin paranodal loop membrane protein Opalin in the mouse brain. (2014)
  • Author(s): Yumi Sato, Fumio Yoshikawa, Tetsushi Sadakata, Yo Shinoda, Michinori Koebis, Teiichi Furuichi.
  • Journal Title: Neurosci Letter Volume: 581 Pages: 14-19
  • DOI: 10.1016/j.neulet.2014.08.017
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] BDNF enhances spontaneous and activity-dependent neurotransmitter release at excitatory terminals but not at inhibitory terminals in cultured hippocampal neurons. (2014)
  • Author(s): Shinoda Y, Ahmed S, Ramachandran B, Bharat V, Brockelt D, Altas B, Dean C
  • Journal Title: Front. Synaptic Neurosci Volume: 6 Pages: 1-12
  • DOI: 10.3389/fnsyn.2014.00027
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Autistic-like behavioral phenotypes in a mouse model with copy number variation of the CAPS2/CADPS2 gene (2013)
  • Author(s): Sadakata T, Shinoda Y, Oka M, Sekine Y, Furuichi T.
  • Journal Title: FEBS Lett Volume: 587 Issue: 1 Pages: 54-59
  • DOI: 10.1016/j.febslet.2012.10.047
  • Peer Reviewed
• [Journal Article] Animal Models of Autism Spectrum Disorder (ASD): A Synaptic-Level Approach to Autistic-Like Behavior in Mice (2013)
  • Author(s): Shinoda Y, Sadakata T, Furuichi T
  • Journal Title: Experimental Animals Volume: 62 Issue: 2 Pages: 71-78
  • DOI: 10.1538/expanim.62.71
  • NAID: 10031163545
  • ISSN: 0007-5124, 1341-1357, 1881-7122
  • Peer Reviewed
• [Journal Article] CAPS1 deficiency perturbs dense-core vesicle trafficking and Golgi structure and reduces presynaptic release probability in the mouse brain. (2013)
  • Author(s): Sadakata T, Kakegawa W, Shinoda Y, Hosono M, Katoh-Semba R, Sekine Y, Sato Y, Tanaka M, Iwasato T, Itohara S, Furuyama K, Kawaguchi Y, Ishizaki Y, Yuzaki M, Furuichi T.
  • Journal Title: J Neurosci. Volume: 33 Issue: 44 Pages: 17326-17334
  • DOI: 10.1523/jneurosci.2777-13.2013
  • Peer Reviewed
• [Presentation] Brain-specific expression of lysosomal-associated membrane protein 5 (LAMP5) gene (2014)
  • Author(s): Michinori Koebis, Yuzuru Saito, Yo Shinoda, Teiichi Furuichi
  • Organizer: Society for Neuroscience 44st Annual Meeting
  • Place of Presentation: Washington DC, US
  • Year and Date: 2014-11-17
• [Presentation] Age-dependent redistribution and hypersialylation of the central myelin loop membrane protein Opalin in the mouse brain (2014)
  • Author(s): T. FURUICHI, Y. SATO, F. YOSHIKAWA, T. SADAKATA, Y. SHINODA, M. KOEBIS.
  • Organizer: Society for Neuroscience 44st Annual Meeting
  • Place of Presentation: Washington DC, US
  • Year and Date: 2014-11-16
• [Presentation] Tutorial contents on the INCF japan node platforms (2014)
  • Author(s): Y. YAMAGUCHI, S. SATOH, T. IIJIMA, R. KANZAKI, T. FURUICHI, Y. SHINODA, S. KAKEI, S. MASAKI, H. WAGATSUMA, T. MIYAKAWA, K. TAKAO, H. IKENO, K. TANAKA, Y. OKAMURA-OHO, Y.OKUMURA, S. KAMAKURA1, Y. ISONO, Y. MORII, S. SUENAGA, S. USUI.
  • Organizer: Society for Neuroscience 44st Annual Meeting
  • Place of Presentation: Washington DC, US
  • Year and Date: 2014-11-15
• [Presentation] BDNFの分泌と自閉症 (2014)
  • Author(s): 定方哲史、篠田陽、古市貞一
  • Organizer: 第36回日本生物学的精神医学会・第57回日本神経化学会大会
  • Place of Presentation: 奈良：奈良県文化会館・奈良県新公会堂
  • Year and Date: 2014-10-01
  • Invited
• [Presentation] Cerebellar Development Transcriptome Database (CDT-DB) - Profiling of Spatio-Temporal Gene Expression During Postnatal Development of Mouse Brain. (2014)
  • Author(s): Yo Shinoda, Akira Sato, Michinori Koebis, Tetsushi Sadakata, Noriyuki Morita, Michisuke Yuzaki, Takafumi Inoue, Hirozumi Nishibe, Yoko Yamaguchi, Teiichi Furuichi.
  • Organizer: INCF Japan Node International Workshop, Advances in Neuroinformatics 2014
  • Place of Presentation: 埼玉 和光市：理研BSI
  • Year and Date: 2014-09-26
• [Presentation] 成体ニューロン新生におけるCAPS2の役割 (2014)
  • Author(s): 柳下香織、篠田陽、古市貞一
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: 神奈川；パシフィコ横浜
  • Year and Date: 2014-09-13
• [Presentation] シナプス小胞と有芯小胞における同時イメージング解析 (2014)
  • Author(s): 中島柚依、篠田陽、古市貞一
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: 神奈川：パシフィコ横浜
  • Year and Date: 2014-09-12
• [Presentation] 長期増強のシナプス特異性に寄与するプレシナプスタグの探求 (2014)
  • Author(s): 齊藤新平、篠田陽、定方哲史、西岡朋生、貝淵弘三、古市貞一
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: 神奈川：パシフィコ横浜
  • Year and Date: 2014-09-12
• [Presentation] 小脳におけるLAMP5の発現 (2014)
  • Author(s): 古戎道則、齊藤由弦、篠田陽、古市貞一
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: 神奈川：パシフィコ横浜
  • Year and Date: 2014-09-11
• [Presentation] シナプトタグミン３：ポストシナプスタンパク質の表面発現制御因子 (2013)
  • Author(s): Yo Shinoda, Saheeb Ahmed, Reimi Abe, Ankit Awasthi, Teiichi Furuichi, Camin Dean
  • Organizer: 第36回日本神経科学大会
  • Place of Presentation: 京都
• [Presentation] マウスドーパミン作動性神経の初代培養系における大型有芯小胞からのドーパミン分泌とCAPS2の関係 (2013)
  • Author(s): 井口大壽、小林翔太、定方哲史、篠田陽、古市貞一
  • Organizer: 第36回日本神経科学大会
  • Place of Presentation: 京都
• [Presentation] CAPS1コンディショナル・ノックアウトマウスにおけるシナプス伝達 (2013)
  • Author(s): 石井千晶、篠田陽、定方哲史、古市貞一
  • Organizer: 第36回日本神経科学大会
  • Place of Presentation: 京都
• [Presentation] Synaptotagmin3: Potential molecule to regulate surface expression level of synaptic proteins (2013)
  • Author(s): Yo Shinoda, Saheeb Ahmed, Reimi Abe, Ankit Awasthi, Teiichi Furuichi, and Camin Dean
  • Organizer: 包括脳ネットワーク夏のワークショップ
  • Place of Presentation: 名古屋
• [Presentation] Ca2+依存性分泌関連タンパク質CAPSファミリーのマウス胚脳神経系における遺伝子発現 (2013)
  • Author(s): 三島百合子、須田翔子、猿田千尋、定方哲史、篠田陽、和田直之、小島正巳、若菜茂晴、古市貞一
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 神奈川
• [Presentation] A synaptotagmin enriched in the post-synaptic membrane is involved in receptor recycling (2013)
  • Author(s): A. AWASTHI, S. AHMED, Y. SHINODA, H. MARTENS, B. COOPER, F. GETTFERT, S. W. HELL, C. DEAN
  • Organizer: Neuroscience 2013
  • Place of Presentation: San Diego, CA, US
• [Book] 自閉症の今 (2013)
  • Author(s): 篠田陽
  • Total Pages: 5
  • Publisher: 科学フォーラム