| Project/Area Number |
25860174
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General physiology
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| Research Institution | National Cardiovascular Center Research Institute |
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Principal Investigator |
NAKAO Shu 国立研究開発法人国立循環器病研究センター, 研究所, 流動研究員 (30646956)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAMURA Tomoe Y 国立研究開発法人国立循環器病研究センター, 研究所, 室長 (50393244)
WAKABAYASHI Shigeo 国立研究開発法人国立循環器病研究センター, 研究所, 非常勤研究員 (70158583)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 細胞生物学 / 分子生物学 / カルシウム / 心肥大 / 心筋細胞 / カルシウムセンサー / 興奮収縮連関 / 受容体刺激 / 細胞内シグナル伝達 / イノシトール三リン酸受容体 |
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| Outline of Final Research Achievements |
To investigate the regulation of nuclear calcium in cardiomyocytes, we generated adenovirus vectors of cytoplasmic/nuclear-specific calcium probes GECOs, and induced them in mouse ventricular myocytes. Calcium imaging analysis revealed that a hypertrophy-associated hormone induced larger calcium transients in the nucleus than those in the cytoplasm. Using mice lacking a calcium sensor protein neuronal calcium censor-1 (NCS-1), which we previously reported as a crucial regulator of calcium signaling in cardiomyocytes, we demonstrated that NCS-1 contributes to the nuclear calcium regulation. Moreover, a protein/protein interaction assay exhibited that a NCS-1-dependent regulation of nuclear calcium is modulated through the interaction between NCS-1 and inositol-1,4,5 trisphosphate receptor, an intracellular calcium release channel, in cardiomyocytes.
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