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The mechanisms of Nrf2-HO-1 activation and cytoprotective effects induced by flavonoids

Research Project

Project/Area Number 25860187
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field General pharmacology
Research InstitutionNagoya University

Principal Investigator

MATSUSHIMA Miyoko  名古屋大学, 医学(系)研究科(研究院), 助教 (10509665)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsフラボノイド / Nrf2 / heme oxygenase-1 / heme oxygenase
Outline of Final Research Achievements

Flavonoids are known to exhibit various cytoprotective effects. In this study, we investigated the mechanisms of Nrf2 activation and cytoprotective effects induced by flavonoids. We found the chrysin, one of the flavonoids, had cytoprotective effect without HO-1 induction. We compared the effect of quercetin, which can induce HO-1, and chrysin on the inhibition of mast cell activation, and suggested that HO-1 might regulate signaling events before calcium mobilization associated with mast cell activation. We also focused on caveolin-1 (Cav-1), with which Nrf2 interacted in cell membrane to regulate cellular anti-oxidant capacity. We showed that quercetin changed the formation of lipid rafts, and the expression level of Cav-1 and Nrf2 was decreased in cell membrane and increased in cytosol and nucleus after exposure to quercetin. These results suggested that the disruption of lipid rafts would translocate Cav-1 and Nrf2 from cell membrane and to nucleus, leading to HO-1 induction.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (20 results)

All 2014 2013 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (17 results) Remarks (1 results)

  • [Journal Article] Protective effects of intratracheally administered quercetin on lipopolysaccharide-induced acute lung injury.2014

    • Author(s)
      Takashima K, Matsushima M, Hashimoto K, Nose H, Sato M, Hashimoto N, Hasegawa Y, Kawabe T.
    • Journal Title

      Respir Res.

      Volume: 15 Issue: 1 Pages: 150-150

    • DOI

      10.1186/s12931-014-0150-x

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Micromachined catheter flow sensor and its applications in breathing measurements in animal experiments.2014

    • Author(s)
      Shikida M, Shikano T, Matsuyama T, Yamazaki Y, Matsushima M, Kawabe T
    • Journal Title

      Microsyst Technol.

      Volume: 20 Issue: 3 Pages: 505-513

    • DOI

      10.1007/s00542-013-1855-3

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] The involvement of cell membrane changes in quercetin-induced cytoprotective effects.2014

    • Author(s)
      Kodera Y, Matsushima M, Shiga M, Oomura A, Ogiso H, Kawabe T.
    • Organizer
      第43回日本免疫学会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Year and Date
      2014-12-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] Different mechanism of cytoprotective effects by flavonoids, quercetin and chrysin.2014

    • Author(s)
      Shiga M, Matsushima M, Kodera Y, Oomura A, Ogiso H, Kawabe T.
    • Organizer
      第43回日本免疫学会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Year and Date
      2014-12-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] 肥満細胞活性化に対するクリシンとケルセチンの抑制効果とその機序の相違2014

    • Author(s)
      大村 綾、松島充代子、志賀茉梨化、草次裕人、小笠原名奈子、武村和哉、小寺佑果、小木曽寛希、川部 勤
    • Organizer
      第9回日本臨床検査学教育学会学術大会
    • Place of Presentation
      大田区産業プラザ(東京都)
    • Year and Date
      2014-08-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] TLR7を介した肺胞マクロファージの活性化に対するケルセチンの効果2014

    • Author(s)
      小寺佑果、松島充代子、小笠原名奈子、武村和哉、志賀茉梨化、大村 綾、小木曽寛希、川部 勤
    • Organizer
      第9回日本臨床検査学教育学会学術大会
    • Place of Presentation
      大田区産業プラザ(東京都)
    • Year and Date
      2014-08-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] Inhibitory action of mast cell degranulation by chrysin2014

    • Author(s)
      Shiga M, Matsushima M, Kusatsugu Y, Ogasawara N, Takemura K, Kodera Y, Oomura A, Ogiso H, Kawabe T.
    • Organizer
      The 24th Congress of Interasma Japan / North Asia
    • Place of Presentation
      ウインクあいち(愛知県)
    • Year and Date
      2014-07-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Effects of quercetin on TLR7-mediated macrophage activation.2014

    • Author(s)
      Oomura A, Matsushima M, Ogasawara N, Takemura K, Kodera Y, Shiga M, Ogiso H, Kawabe T.
    • Organizer
      The 24th Congress of Interasma Japan / North Asia
    • Place of Presentation
      ウインクあいち(愛知県)
    • Year and Date
      2014-07-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 非侵襲的治療薬物モニタリングのための呼気用新規吸着剤の開発2014

    • Author(s)
      日置清香、三輪俊夫、山本良平、井上嘉則、山本 敦、松島充代子、川部 勤
    • Organizer
      日本薬学会第134年会
    • Place of Presentation
      熊本市総合体育館(熊本県)
    • Related Report
      2013 Research-status Report
  • [Presentation] The effect of quercetin on the activated alveolar macrophages.2013

    • Author(s)
      Haruka Nose, Miyoko Matsushima, Aya Oomura, Kanae Mori, Nanako Ogasawara, Kazuya Takemura, Takehiro Yamaguchi, Yuka Kodera, Marika Shiga, Tsutomu Kawabe.
    • Organizer
      ICI 2013
    • Place of Presentation
      MiCo Milano Congressi (Italy)
    • Related Report
      2013 Research-status Report
  • [Presentation] Difference in reaction patterns of M1 and M2 macrophages induced by quercetin.2013

    • Author(s)
      Miyoko Matsushima, Aya Oomura, Kanae Mori, Nanako Ogasawara, Kazuya Takemura, Haruka Nose, Takehiro Yamaguchi, Yuka Kodera, Marika Shiga, Tsutomu Kawabe.
    • Organizer
      ICI 2013
    • Place of Presentation
      MiCo Milano Congressi (Italy)
    • Related Report
      2013 Research-status Report
  • [Presentation] Investigation on frequency of mutation in the gene segments of immunoglobulin in CD40 knockout mice.2013

    • Author(s)
      Yuka Kodera, Miyoko Matsushima, Hiroki Ogiso, Nanako Ogasawara, Kazuya Takemura, Haruka Nose, Takehiro Yamaguchi, Marika Shiga, Tsutomu Kawabe
    • Organizer
      ICI 2013
    • Place of Presentation
      MiCo Milano Congressi (Italy)
    • Related Report
      2013 Research-status Report
  • [Presentation] Chrysin regulates mast cell activation via HO-1-independent pathway.2013

    • Author(s)
      Marika Shiga, Miyoko Matsushima, Ayaka Teranishi, Haruka Nose, Nanako Ogasawara, Kazuya Takemura, Takehiro Yamaguchi, Yuka Kodera, Tsutomu Kawabe.
    • Organizer
      ICI 2013
    • Place of Presentation
      MiCo Milano Congressi (Italy)
    • Related Report
      2013 Research-status Report
  • [Presentation] 環境物質に対するフラボノイドの細胞保護効果とその機序2013

    • Author(s)
      松島充代子、小笠原名奈子、武村和哉、野瀬遥加、山口剛広、小寺佑果、志賀茉梨花、上山 純、川部 勤
    • Organizer
      第44回日本職業・環境アレルギー学会総会・学術大会
    • Place of Presentation
      小田急ホテルセンチュリー相模大野(神奈川県)
    • Related Report
      2013 Research-status Report
  • [Presentation] 正常・疾患ラット肺組織の3次元超高分解能OCTイメージング2013

    • Author(s)
      服部雄治、川越寛之、石田周太郎、荒巻光利、西澤典彦、松島充代子、高嶋浩司、長谷川好規、川部勤
    • Organizer
      日本光学会年次学術講演会Optics & Photonics Japan 2013
    • Place of Presentation
      奈良県新公会堂(奈良県)
    • Related Report
      2013 Research-status Report
  • [Presentation] 環境物質曝露によるマクロファージの活性化に対するフラボノイドの効果とその機序2013

    • Author(s)
      山口剛広、松島充代子、川部 勤.
    • Organizer
      第63回日本アレルギー学会秋季学術大会
    • Place of Presentation
      ホテルニューオータニ(東京都)
    • Related Report
      2013 Research-status Report
  • [Presentation] TLR7を介した肺胞マクロファージの活性化に対するケルセチンならびにその誘導するHO -1の効果について2013

    • Author(s)
      安井正宏、松島充代子、川部 勤
    • Organizer
      第63回日本アレルギー学会秋季学術大会
    • Place of Presentation
      ホテルニューオータニ(東京都)
    • Related Report
      2013 Research-status Report
  • [Presentation] Chrysin suppresses mast cell activation via HO-1-independent pathway.2013

    • Author(s)
      Marika Shiga, Miyoko Matsushima, Haruka Nose, Takehiro Yamaguchi, Yuka Kodera, Tsutomu Kawabe
    • Organizer
      第42回日本免疫学会
    • Place of Presentation
      幕張メッセ(千葉県)
    • Related Report
      2013 Research-status Report
  • [Presentation] The inhibitory effect of quercetin on TLR7-induced activation of alveolar macrophages.2013

    • Author(s)
      Yuka Kodera, Miyoko Matsushima, Haruka Nose, Takehiro Yamaguchi, Marika Shiga, Tsutomu Kawabe
    • Organizer
      第42回日本免疫学会
    • Place of Presentation
      幕張メッセ(千葉県)
    • Related Report
      2013 Research-status Report
  • [Remarks] 研究室ホームページ

    • URL

      http://square.umin.ac.jp/kawabe/index.html

    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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