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Time-dependent Changes of PFDN5 Expression during a Long-term Potentiation-like Facilitation in Adult Mouse Hippocampus Induced by Nicotine Application

Research Project

Project/Area Number 25860196
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field General pharmacology
Research InstitutionHimeji Dokkyo University

Principal Investigator

Matsuura Kenji  姫路獨協大学, 薬学部, 助手 (20452641)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywordsシナプス可塑性 / プレフォルディン / ニコチン / ユビキチン‐プロテアソーム系 / ニコチン受容体 / 海馬 / プロテオミクス / LTP様作用
Outline of Final Research Achievements

Long-lasting synaptic plasticity requires changes of protein expression, although the mechanisms remain unclear. We previously reported that nicotine expressed a long-term potentiation-like facilitation, that is synaptic plasticity, in mouse hippocampus. In this study, we conducted to clarify the involvement of Prefoldin subunit 5 (PFDN5) in synaptic plasticity.After nicotine treatment (3 mg/kg, i.p.), mRNA and protein expression of PFDN5 in hippocampus increased gradually during 2-24-hr period. This enhanced expression of PFDN5 protein at 24-hr was not completely inhibited by pretreatment of mecamylamine (0.5 mg/kg, i.p.), a nonselective nicotinic ACh receptor (nAChR) antagonist. Ubiquitinated PFDN5 increased during synaptic plasticity after nicotine treatment.These findings demonstrated that PFDN5 protein was enhanced during synaptic plasticity through coordinated regulation of gene expression and protein degradation.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (8 results)

All 2016 2015 2013

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Acknowledgement Compliant: 3 results) Presentation (3 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] The influence of chronic nicotine treatment on proteins expressed in the mouse hippocampus and cortex.2016

    • Author(s)
      Kenji Matsuura, Mieko Otani, Masaoki Takano, Keiichi Kadoyama, Shogo Matsuyama.
    • Journal Title

      European Journal of Pharmacology

      Volume: 780 Pages: 16-25

    • DOI

      10.1016/j.ejphar.2016.03.025

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Changes in the expression of collapsin response mediator protein-2 during synaptic plasticity in the mouse hippocampus.2015

    • Author(s)
      Keiichi Kadoyama, Kenji Matsuura, Tooru Nakamura-Hirota, Masaoki Takano, Mieko Otani, Shogo Matsuyama.
    • Journal Title

      Journal of Neuroscience Research

      Volume: 93 Issue: 11 Pages: 1684-1692

    • DOI

      10.1002/jnr.23626

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] The influence of chronic ibuprofen treatment on proteins expressed in the mouse hippocampus.2015

    • Author(s)
      Kenji Matsuura, Mieko Otani, Masaoki Takano, Keiichi Kadoyama, Shogo Matsuyama
    • Journal Title

      European Journal of Pharmacology

      Volume: 752 Pages: 61-68

    • DOI

      10.1016/j.ejphar.2015.01.047

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Proteomic analysis of time-dependent changes in proteins expressed in mouse hippocampus during synaptic plasticity induced by GABAA receptor blockade.2013

    • Author(s)
      Matsuura K, Nakamura-Hirota T, Takano M, Otani M, Kadoyama K, Matsuyama S
    • Journal Title

      Neuroscience Letters

      Volume: 555 Pages: 18-23

    • DOI

      10.1016/j.neulet.2013.07.036

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Proteomic analysis of the hippocampus in Alzheimer's disease model mice by using two-dimensional fluorescence difference in gel electrophoresis2013

    • Author(s)
      Takano M., Yamashita T., Nagano K., Otani M., Maekura K., Kamada H., Tsunoda S., Tsutsumi Y., Tomiyama T., Mori H., Matsuura K., Matsuyama S
    • Journal Title

      Neuroscience Letters

      Volume: 534 Pages: 85-9

    • DOI

      10.1016/j.neulet.2012.11.010

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Proteomic analysis of the hippocampus and cerebral cortex of streptozotocin-treated mice showing cognitive dysfunction2016

    • Author(s)
      K. Matsuura, M. Otani, M. Takano, K. Kadoyama, S. Matsuyama
    • Organizer
      第89回日本薬理学会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2016-03-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] The influence of chronic nicotine treatment on proteins expressed in the mouse hippocampus and cortex2015

    • Author(s)
      K. Matsuura, M. Otani, M. Takano, K. Kadoyama, S. Matsuyama
    • Organizer
      International Society for Neurochemistry
    • Place of Presentation
      Cairns Convention Centre (Australia)
    • Year and Date
      2015-08-26
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Time-dependent changes of PFD5 expression during a long-term potentiation-like facilitation in adult mouse hippocampus induced by nicotine application.2015

    • Author(s)
      Kenji Matsuura, Keiichi Kadoyama, Masaoki Takano, Mieko Otani, and Shogo Matsuyama
    • Organizer
      第88回 日本薬理学会年会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2015-03-20
    • Related Report
      2014 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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