Emerging role of enhancer RNA in NRF2-dependent human HO-1 gene induction
Project/Area Number |
25860202
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
General medical chemistry
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Research Institution | Hirosaki University |
Principal Investigator |
MARUYAMA Atsushi 弘前大学, 医学(系)研究科(研究院), 助教 (10431438)
|
Project Period (FY) |
2013-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | ヘムオキシゲナーゼー1 / NRF2 / ノンコーディングRNA / 転写制御 / 酸化ストレス / ヘムオキシゲナーゼ / Nrf2 / エピジェネティクス |
Outline of Final Research Achievements |
Emerging evidence indicates that enhancer RNAs (eRNAs), which are long non-coding RNAs transcribed from gene enhancer regions, play important roles in the regulation of gene expression. However, it remains unknown whether eRNAs are involved in the human heme oxygenase-1 gene (HO-1) induction. HO-1 possesses two distantly located enhancers called E2 and E1 enhancers to regulate its transcription. In this study, I demonstrated that eRNAs are transcribed from the human HO-1 enhancers. Focusing on E2-derived enhancer RNA (eRNA E2), I found eRNA E2 is induced in response to HO-1 substrate heme and oxidative stress-causing reagents such as diethyl maleate (DEM) in an NRF2-dependent manner. I also found DEM-induced HO-1 expression was selectively down-regulated by knockdown of the eRNA E2. Furthermore, DEM-induced Pol II binding to human HO-1 regions is attenuated in eRNA E2 knockdown cells. These results indicate that eRNA E2 is functional and required for the induction of HO-1.
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Report
(3 results)
Research Products
(16 results)
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[Journal Article] p62 Deficiency Enhances α-Synuclein Pathology in Mice.2015
Author(s)
Tanji K, Odagiri S, Miki Y, Maruyama A, Nikaido Y, Mimura J, Mori F, Warabi E, Yanagawa T, Ueno S, Itoh K, Wakabayashi K.
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Journal Title
Brain Pathol.
Volume: 印刷中
Issue: 5
Pages: 552-564
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Nrf2- and ATF4-dependent up-regulation of xCT modulates the sensitivity of T24 bladder carcinoma cells to proteasome inhibition.2014
Author(s)
Peng Ye, P., Mimura J., Okada, T., Sato, H., Liu, T., Maruyama, A., Ohyama, C., and Itoh, K.
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Journal Title
Mol. Cell. Biol.
Volume: 34
Issue: 18
Pages: 3421-3434
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Phosphorylation of serine 349 of p62 in Alzheimer’s disease brain2014
Author(s)
Tanji K, Miki Y, Ozaki T, Maruyama A, Yoshida H, Mimura J, Matsumiya T, Mori F, Imaizumi T, Itoh K, Kakita A, Takahashi H, Wakabayashi K
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Journal Title
Acta Neuropathol Comm
Volume: 2
Issue: 1
Pages: 50-50
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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