| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Heme binds to proteins to modulate their function. However, the regulatory role of heme in the innate immune responses has been unknown. Previous study we found that heme binds to transcription repressor Bach2 to inhibit its DNA binding activity and to induce its degradation in B cells. On the basis of this study we focused on the regulatory of heme in the innate immune response. Firstly, we compared red pulp macrophage phenotype between wild-type and Bach2 knockout mice by using DNA microarray analysis. In this macrophage, there were almost no change between wild-type and Bach2 knock out mice in gene expressions. Secondly, we performed DNA microarray by using Bach2 reporter mice bone marrow macrophage. The results of the gene ontology analysis of the Bach2 expressed-cells showed “ heme biosynthetic process” and “ heme catabolic process”. These results suggested that Bach2 may integrate heme metabolism and synthesis in bone marrow macrophage.
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