Wnt signaling promotes epithelial tubulogenesis through the regulation of cell morphology and proliferation
| Project/Area Number |
25860211
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
MATSUMOTO Shinji 大阪大学, 医学(系)研究科(研究院), 特任助教(常勤) (20572324)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | Wnt / EGF / Arｌ４ｃ / YAP/TAZ / 上皮管腔形成 / Arl4c / 上皮分岐管腔形成 |
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| Outline of Final Research Achievements |
Co-stimulation with Wnt3a and EGF induced development of tubes consisting of rat intestinal epithelial cells (IEC6) by inducing expression of Arl4c in three-dimensional culture. Arl4c expression resulted in cell morphological change (extension) through the activation of Rac and inactivation of Rho properly, which promoted cell growth by inducing nuclear translocation of YAP/TAZ in leading cells. Arl4c was expressed in ureteric bud tips in the embryonic kidney and regulated ureteric buds tubulogenesis. Furthermore, immunohistochemical analyses revealed that Arl4c was strongly expressed at high frequencies in tumor lesions of colorectal and lung cancers. Arl4c was involved in migration, invasion and proliferation capabilities both in vitro and in vivo.
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Report
(3 results)
Research Products
(15 results)
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[Journal Article] A. Basolateral secretion of Wnt5a in polarized epithelial cells is required for apical lumen formation2015
Author(s)
Yamamoto, H., Awada, C., Matsumoto, S., Kaneiwa, T., Sugimoto, T., Takao, T., and Kikuchi
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Journal Title
J. Cell Sci.
Volume: 128
Pages: 1051-1063
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] A combination of Wnt and growth factor signaling induces Arl4c expression to form epithelial tubular structures.2014
Author(s)
Matsumoto, S., Fujii, S., Sato, A., Ibuka, S., Kagawa, Y., Ishii, M., and Kikuchi, A.
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Journal Title
EMBO J
Volume: 33
Issue: 7
Pages: 702-718
DOI
Related Report
Peer Reviewed
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[Journal Article] Cell cycle-dependent Rho GTPase activity dynamically regulates cancer cell motility and invasion in vivo.2014
Author(s)
Kagawa Y, Matsumoto S, Kamioka Y, Mimori M, Naito Y, Ishii T, Okuzaki D, Nishida N, Maeda S, Naito A, Kikuta J, Nishikawa K, Nishimura J, Haraguchi N, Takemasa I, Mizushima T, Ikeda M, Yamamoto H, Sekimoto M, Ishii H, Doki Y, Matsuda M, Kikuchi A, Mori M, Ishii M
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Journal Title
PLoS One
Volume: 8
Issue: 12
Pages: e83629-e83629
DOI
Related Report
Peer Reviewed / Open Access
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