Transdifferentiation of pancreatic exocrine cells to beta cells
| Project/Area Number |
25860213
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
MIYAZAKI Satsuki 大阪大学, 医学(系)研究科(研究院), 助教 (60452439)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 転写因子 / 腺房細胞 / インスリン / インスリン産生細胞 / 分化転換 |
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| Outline of Final Research Achievements |
I established a transgenic mouse line in which Pdx1 and Isl1 could be inducibly expressed in the acinar cells. My results showed that acinar-derived EGFP-positive insulin producing cells appear by induction of both Pdx1 and Isl1 in adult exocrine cells. Overexpression of the factors necessary for transdifferentiaion using lentivirul vectors in sorted EGFP-positive acinar cells induced acinar-to-beta cell transdifferentiation. Although further study is required, my result provides a promise for the in vitro transdifferentiation of exocrine cells to beta cells.
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Report
(3 results)
Research Products
(6 results)
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[Journal Article] Expansion and conversion of human pancreatic ductal cells into insulin-secreting endocrine cells.2013
Author(s)
Lee J, Sugiyama T, Liu Y, Wang J, Gu X, Lei J, Markmann JF, Miyazaki S, Miyazaki J, Szot GL, Bottino R, Kim SK
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Journal Title
DOI
Related Report
Peer Reviewed
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