Transdifferentiation of pancreatic exocrine cells to beta cells

• Project/Area Number: 25860213
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Osaka University
• Principal Investigator: MIYAZAKI Satsuki 大阪大学, 医学(系)研究科(研究院), 助教 (60452439)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 転写因子 / 腺房細胞 / インスリン / インスリン産生細胞 / 分化転換

Outline of Final Research Achievements

I established a transgenic mouse line in which Pdx1 and Isl1 could be inducibly expressed in the acinar cells. My results showed that acinar-derived EGFP-positive insulin producing cells appear by induction of both Pdx1 and Isl1 in adult exocrine cells. Overexpression of the factors necessary for transdifferentiaion using lentivirul vectors in sorted EGFP-positive acinar cells induced acinar-to-beta cell transdifferentiation. Although further study is required, my result provides a promise for the in vitro transdifferentiation of exocrine cells to beta cells.

Research Products

• [Journal Article] エレクトロポレーションによる生体への遺伝子導入 (2014)
  • Author(s): 宮崎 早月、宮崎 純一
  • Journal Title: Surgery Frontier Volume: 21 Pages: 62-66
• [Journal Article] Functional analysis of Tcl1 using Tcl1-deficient mouse embryonic stem cells. (2013)
  • Author(s): Miyazaki T, Miyazaki S, Ashida M, Tanaka T, Tashiro F, Miyazaki J
  • Journal Title: PLoS One Volume: 8 Issue: 8 Pages: e71645-e71645
  • DOI: 10.1371/journal.pone.0071645
  • Peer Reviewed
• [Journal Article] Expansion and conversion of human pancreatic ductal cells into insulin-secreting endocrine cells. (2013)
  • Author(s): Lee J, Sugiyama T, Liu Y, Wang J, Gu X, Lei J, Markmann JF, Miyazaki S, Miyazaki J, Szot GL, Bottino R, Kim SK
  • Journal Title: Elife Volume: 2
  • DOI: 10.7554/elife.00940
  • Peer Reviewed
• [Presentation] Ces1遺伝子欠損マウス胎仔におけるヒストンH3K9及びDNAメチル化レベルの亢進と胎生致死・成長遅延および始原生殖細胞の分化異常 (2014)
  • Author(s): 松浦 巧、宮崎 竜志、宮崎 早月、田代 文、宮崎 純一
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Ces1遺伝子欠損マウスにみられる始原生殖細胞の生存・増殖・分化の異常 (2013)
  • Author(s): 松浦 巧、宮崎 竜志、宮崎 早月、田代 文、宮崎 純一
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸ポートアイランド
• [Book] 「医学のための生命科学」 第１０章 再生 (2014)
  • Author(s): 宮崎 早月、宮崎 純一
  • Total Pages: 300
  • Publisher: 南山堂