| Project/Area Number |
25860218
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | Tottori University (2014-2016)
Fukushima Medical University (2013) |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | SNAREタンパク / 膜融合 / ファゴサイトーシス / ファゴソーム / マクロファージ / リン酸化 / 膜輸送 / FRET解析 / SNAREタンパク質 |
|---|
| Outline of Final Research Achievements |
Phagocytosis is the one of biological defense mechanisms that are characteristic of phagocytes such as dendritic cells and macrophages, and a reaction to isolate pathogens by surrounding them as phagosomes to sterilize and digest them. Phagocytic processing is composed of phagosome formation (uptake activity) and maturation, and known to go along by a series of complicated membrane fusion of intracellular organelles.
SNAP-23, a plasma membrane-localized SNARE protein which operates membrane fusion events, is involved in phagosome formation and maturation, however, the regulatory mechanism remains totally obscure. In this study, we showed that phagocytosis efficiency is decreased by phosphorylation of SNAP-23 at Ser95 in macrophages. Further, we found that IKK2 is the one of phosphorylation kinases of SNAP-23 at Ser95 on phagosome membrane.
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