| Project/Area Number |
25860219
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | Nagoya City University |
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Principal Investigator |
KEIKO Kono 名古屋市立大学, 医学(系)研究科(研究院), 講師 (30632723)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 細胞創傷治癒 / 分裂老化 / 出芽酵母 / 細胞老化 / 細胞・組織 / ストレス / シグナル伝達 / タンパク質 |
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| Outline of Final Research Achievements |
Cellular wound-healing, enabling the repair of plasma membrane damage, is frequent in nature. Despite its ubiquitous usage, the underlying molecular mechanisms are poorly understood. Here we report a genome-wide screen to identify biological processes required for cellular wound-healing in yeast. 109 out of 6,032 mutations screened exhibited cellular wounding-specific lethality. Based on the screen, we uncovered a previously unappreciated link between cellular wound-healing and replicative aging: senescence determined by the number of cell division. Plasma membrane damage and subsequent damage repair promoted reorganization of phospholipids near the damage site. The plasma membrane reorganization accelerated replicative aging. Thus, we propose that cellular wound-healing mediated PS exposure on the plasma membrane outer leaflet is an evolutionary conserved mechanism promoting cellular senescence in eukaryotes.
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