In vitro derivation of follicles from mouse ES cells and somatic cells
| Project/Area Number |
25860222
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
General medical chemistry
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | ES細胞 / iPS細胞 / 生殖細胞 / 卵胞形成 / 分化誘導 / 卵子形成 / 転写因子 |
|---|
| Outline of Final Research Achievements |
Folliculogenesis-competent ES cells higly expressed transcription factors which are important for folliculogenesis. Based on this finding, we transduced transcription factors essential for folliculogenesis into mouse iPS cells with germ cell-specific reporter gene. Among genes transduced, overexpression of Nobox and Figla induced derivation of germ cell-specific reporter+ cells. The induction was observed even when only Nobox was transduced; however, the efficiency was improved by a combination with Figla. Unexpectedly, overexpression of Nobox and Figla did not induce germ cell-specific reporter+ cells under cell differentiation culture conditions. Taken all together, we speculate that Nobox and Figla would bring pluripotent stem cells to 'metastable state' between pluripotency and germ cells, therefore the cells could turn to somatic cells under somatic cell differentiation cultures.
|
Report
(3 results)
Research Products
(22 results)
-
[Journal Article] Gene expression ontogeny of spermatogenesis in the marmoset uncovers primate characteristics during testicular development.2015
Author(s)
Yu-Ching Lin Z, Hirano T, Shibata S, Seki NM, Kitajima R, Sedohara A, Siomi MC, Sasaki E, Siomi H, Imamura M, Okano H.
-
Journal Title
Dev Biol.
Volume: -
Issue: 1
Pages: 43-58
DOI
Related Report
Peer Reviewed / Open Access
-
-
[Journal Article] Small RNA profiling and characterization of piRNA clusters in the adult testes of the common marmoset, a model primate.2014
Author(s)
Hirano T, Iwasaki YW, Lin ZY, Imamura M, Seki NM, Sasaki E, Saito K, Okano H, Siomi MC, *Siomi H.
-
Journal Title
RNA
Volume: 20
Issue: 8
Pages: 1223-1237
DOI
Related Report
Peer Reviewed / Open Access
-
-
[Journal Article] Aberrant gene expression and sexually incompatible genomic imprinting in oocytes derived from XY mouse embryonic stem cells in vitro.2013
Author(s)
Nitta M, Imamura M, Inoue Y, Kunitomo Y, Lin ZY, Ogawa T, Yogo K, Ishida-Kitagawa N, Fukunaga N, Okano H, Sato E, Takeya T, Miyoshi J
-
Journal Title
PLoS One
Volume: 8
Issue: 3
Pages: e58555-e58555
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
[Presentation] Developmental dynamics and Culture of Testicular Germ Cells in Common Marmoset Callithrix Jacchus2014
Author(s)
Zachary YC Lin, Masanori Imamura, Takamasa Hirano, Eiji Matsunaga, Miki Taoka, Hiroo Imai, Hirotaka J. Okano, Atsushi Iriki, Mikiko C. Siomi, Haruhiko Siomi, Erika Sasaki, Hirohisa Hirai, Hideyuki Okano
Organizer
Cryopreservation Conference 2014
Place of Presentation
岡崎コンファレンスセンター
Year and Date
2014-10-23 – 2014-10-24
Related Report
-
-
-
-
-
[Presentation] Molecular characterization of proliferative germ-like cells and oocyte-like cells derived from pluripotent stem cells in vitro2013
Author(s)
Masanori Imamura, Mai Nitta, Rieko Ikeda, Keiichiro Yogo, Noritaka Fukunaga, Eimei Sato, Tatsuo Takeya, Jun Miyoshi, Takashi Shinohara, Yasuhisa Matsui, Kuniya Abe, Shinya Yamanaka, Toshiaki Noce, Hideyuki Okano
Organizer
The 11th Stem Cell Research Symposium
Place of Presentation
The University of Tokyo, Ito International Research Center
Related Report
-
-
-
-
-
-
