| Project/Area Number |
25860236
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
WATANABE Akira 京都大学, iPS細胞研究所, 助教 (60506765)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | シングルセル / 脳腫瘍 / 神経分化 / 単一細胞解析 / 細胞分化 |
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| Outline of Final Research Achievements |
The purpose of this study is to address the early event of gliomagenesis. I hypothesized that initiation of tumorigenesis is deviation from a normal neural differentiation. First, I established a methodology of single cell gene expression analysis, which enables to analyze dynamics of transcriptome during the differentiation. By combination with bioinformatical analysis, I successfully re-order the single cell samples in order to differentiation stage. Then, I applied this technique to neural differentiation, and demonstrated the dynamic alteration of transcription during neural differentiation. And also, I could identify cell populations that deviated from normal differentiation. The molecular mechanism that is responsible for branching may facilitate the molecular understanding of gliomagenesis.
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