Molecular understanding of gliomagenesis using neural differentiation sysytem
| Project/Area Number |
25860236
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
WATANABE Akira 京都大学, iPS細胞研究所, 助教 (60506765)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | シングルセル / 脳腫瘍 / 神経分化 / 単一細胞解析 / 細胞分化 |
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| Outline of Final Research Achievements |
The purpose of this study is to address the early event of gliomagenesis. I hypothesized that initiation of tumorigenesis is deviation from a normal neural differentiation. First, I established a methodology of single cell gene expression analysis, which enables to analyze dynamics of transcriptome during the differentiation. By combination with bioinformatical analysis, I successfully re-order the single cell samples in order to differentiation stage. Then, I applied this technique to neural differentiation, and demonstrated the dynamic alteration of transcription during neural differentiation. And also, I could identify cell populations that deviated from normal differentiation. The molecular mechanism that is responsible for branching may facilitate the molecular understanding of gliomagenesis.
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Report
(3 results)
Research Products
(20 results)
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[Journal Article] Dynamic regulation of human endogenous retroviruses mediates factor-induced reprogramming and differentiation potential.2014
Author(s)
Ohnuki M, Tanabe K, Sutou K, Teramoto I, Sawamura Y, Narita M, Nakamura M, Tokunaga Y, Nakamura M, Watanabe A, Yamanaka S, Takahashi K.
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Journal Title
Proceeding of the National Academy of Science U.S.A
Volume: 111
Issue: 34
Pages: 12426-12431
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Expandable Megakaryocyte Cell Lines Enable Clinically Applicable Generation of Platelets from Human Induced Pluripotent Stem Cells.2014
Author(s)
Nakamura S, Takayama N, Hirata S, Seo H, Endo H, Ochi K, Fujita KI, Koike T, Harimoto KI, Dohda T, Watanabe A, Okita K, Takahashi N, Sawaguchi A, Yamanaka S, Nakauchi H, Nishimura S, Eto K.
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Journal Title
Cell Stem Cell.
Volume: 14
Issue: 4
Pages: 535-48
DOI
Related Report
Peer Reviewed / Open Access
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