| Project/Area Number |
25860237
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
MIYATA Yugo 大阪大学, 医学(系)研究科(研究院), 特任研究員 (70623453)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | メタボリックシンドローム / 内臓脂肪細胞 / 前駆脂肪細胞 |
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| Outline of Final Research Achievements |
White adipose tissues (WATs) are classified into visceral and subcutaneous WAT and they have distinct metabolic and biological properties. Many reports have suggested that there are depot specific preadipocytes in each WAT. However, visceral preadipocytes has not been identified until now. In the present study, we aimed to identify preadipocytes from mouse visceral and subcutaneous WATs, and to develop new anti-metabolic syndrome drugs by using mature visceral adipocytes from visceral preadipocytes.
Through in vitro screening and in vivo implantation experiments, visceral preadipocytes were identified. Furthermore, we confirmed that differentiated adipocytes obtained from mesenteric and subcutaneous preadipocytes maintained each characteristic phenotype in vitro as reported in each WAT in vivo. We also developed a simple method for culturing those preadipocytes.
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