| Project/Area Number |
25860239
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Pathological medical chemistry
|
|---|
| Research Institution | Hiroshima University |
|---|
Principal Investigator |
FURUMAI Ryohei 広島大学, 医歯薬保健学研究院, 特任助教 (30450414)
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | ノンコーディングRNA / プラダーウィリー症候群 / 次世代シークエンサー / マイクロRNA |
|---|
| Outline of Final Research Achievements |
Brain-specific non-coding RNA (ncRNA) responsible for the pathogenesis of Prader-Willie syndrome, a rare developmental/mental disorder, was knock-downed in the primary cultured neurons obtained from wild type mouse brain. Then we compared the global transcriptome between control and knock-downed neurons by next-generation sequencer. As a result, we found that the expressions of several genes and miRNAs were significantly changed in knock-downed neurons. Our finding suggested that transcriptional changes in these genes/miRNAs in knock-downed neurons could be related to the etiology of Prader-Willie syndrome.
|
