Analysis of the regulatory mechanism of the human histone methyltransferase SETDB1

• Project/Area Number: 25860253
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pathological medical chemistry
• Research Institution: Osaka University
• Principal Investigator: Tachibana Keisuke 大阪大学, 薬学研究科(研究院), 招へい教員 (30432446)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: ヒストンメチル化酵素 / SETDB1 / 細胞内局在 / 相互作用解析

Outline of Final Research Achievements

SETDB1 is a histone methyltransferase that methylates lysine 9 on histone H3. We found that a post-translational modification of hSETDB1 is important to regulate its methyltransferase activity. Moreover, we identified the regions necessary for interaction between hSETDB1 and hMCAF1. Finally, we showed that nuclear hSETDB1 might be degraded by the proteasome and exported to the cytosol. Theses findings will be useful for regulating hSETDB1 function.

Research Products

• [Journal Article] Analysis of the subcellular localization of the human histone methyltransferase SETDB1 (2015)
  • Author(s): Tachibana K, Gotoh E, Kawamata N, Ishimoto K, Uchihara Y, Iwanari H, Sugiyama A, Kawamura T, Mochizuki Y, Tanaka T, Sakai J, Hamakubo T, Kodama T, Doi T.
  • Journal Title: Biochem Biophys Res Commun Volume: 465 Issue: 4 Pages: 725-731
  • DOI: 10.1016/j.bbrc.2015.08.065
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] プラスチック工業製品中に含まれる難燃剤によるマウス脂肪細胞分化への影響評価 (2014)
  • Author(s): 秋山恵麻, 中西菜月, 角谷秀樹, 中尾晃幸, 高野友希, 橘敬祐, 土井健史, 太田壮一
  • Organizer: 第64回 日本薬学会近畿支部総会・大会
  • Place of Presentation: 京都
  • Year and Date: 2014-10-11
• [Presentation] 母乳汚染物質テトラブロモビスフェノールＡによる脂肪細胞の分化攪乱作用 (2014)
  • Author(s): 秋山恵麻, 空久保良太, 角谷秀樹, 中尾晃幸, 橘敬祐, 土井健史, 太田壮一
  • Organizer: 第64回 日本薬学会近畿支部総会・大会
  • Place of Presentation: 京都
  • Year and Date: 2014-10-11
• [Presentation] ヒストンメチル化酵素 SETDB1 の酵素活性と翻訳後修飾の解析 (2013)
  • Author(s): 川又那津子, 石本憲司, 内原佳恵, 後藤英子, 垣之内啓介, 溝端栄一, 望月康弘, 酒井寿郎, 井上豪, 児玉龍彦, 橘敬祐, 土井健史
  • Organizer: 第63回 日本薬学会近畿支部総会・大会
  • Place of Presentation: 京都
• [Remarks]
  • URL: http://www.phs.osaka-u.ac.jp/homepage/b018/index.html