The roles of EBV-encoded microRNAs in the regulation of apoptosis and carcinogenesis

• Project/Area Number: 25860262
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Human pathology
• Research Institution: The University of Tokyo
• Principal Investigator: SHINOZAKI-USHIKU Aya 東京大学, 医学部附属病院, 特任講師(病院) (60581824)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: Epstein-Barr ウイルス / アポトーシス / 胃癌 / microRNA / EBV / マイクロＲＮＡ

Outline of Final Research Achievements

To clarify the roles of EBV-encoded microRNAs in gastric carcinogenesis, surgically resected gastric carcinoma tissues and gastric carcinoma cell lines were analyzed. Comprehensive expression profiling of EBV-encoded microRNAs revealed several EBV microRNAs were highly expressed. Genes which are potentially targeted by these microRNAs were identified by in silico analysis. In vitro study showed ebv-miR-BART4-5p inhibits Bid, one of the apoptosis activators, which leads to resistance to apoptosis.

Research Products

• [Journal Article] Profiling of Virus-encoded MicroRNAs in Epstein-Barr Virus-associated Gastric Carcinoma and their Roles in Gastric Carcinogenesis. (2015)
  • Author(s): Shinozaki-Ushiku A, Kunita A, Isogai M, Hibiya T, Ushiku T, Takada K, Fukayama M.
  • Journal Title: Journal of Virology Volume: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Update on Epstein-Barr virus and gastric cancer (Review). (2015)
  • Author(s): Shinozaki-Ushiku A, Kunita A, Fukayama M.
  • Journal Title: Int J Oncol Volume: 46 Issue: 4 Pages: 1421-1434
  • DOI: 10.3892/ijo.2015.2856
  • Peer Reviewed
• [Presentation] EBV関連胃癌 (2015)
  • Author(s): 牛久 綾
  • Organizer: 第104回日本病理学会総会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2015-04-30 – 2015-05-02
• [Presentation] Epstein-Barr Virus-encoded MicroRNAs in Gastric Carcinoma (2015)
  • Author(s): Akiko Kunita, Aya Shinozaki-Ushiku, Hiroyuki Abe, Munetoshi Hinata, Masashi Fukayama
  • Organizer: 第5回新学術領域発がんスパイラル国際シンポジウム
  • Place of Presentation: 神戸ポートピアホテル（兵庫県神戸市）
  • Year and Date: 2015-02-26 – 2015-02-27
• [Presentation] EBV関連胃癌におけるウイルス由来microRNAの発現およびその機能の解明 (2014)
  • Author(s): 牛久 綾 国田 朱子 磯貝 まや 牛久 哲男 高田 賢蔵 深山 正久
  • Organizer: 第103回日本病理学会総会
  • Place of Presentation: ANAクラウンプラザホテル広島（広島県広島市）
  • Year and Date: 2014-04-24 – 2014-04-26
• [Presentation] EBV 関連胃癌におけるウイルス由来microRNA の発現およびその機能の解明 (2014)
  • Author(s): 牛久 綾 国田 朱子 磯貝 まや 牛久 哲男 高田 賢蔵 深山 正久
  • Organizer: 第103回日本病理学会総会
  • Place of Presentation: 広島
• [Presentation] Epstein-Barr Virus-encoded MicroRNAs in Gastric Carcinoma (2014)
  • Author(s): 国田 朱子 牛久 綾 阿部 浩幸 磯貝 まや 深山 正久
  • Organizer: 第4回新学術領域発がんスパイラル国際シンポジウム
  • Place of Presentation: 札幌
• [Remarks] 東京大学 医学部 人体病理学・病理診断学
  • URL: http://pathol.umin.ac.jp/index.shtml