| Project/Area Number |
25860275
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | Jichi Medical University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | ノンコーディングRNA / 上皮間葉転換 / 肺腺癌 / non-coding RNA / EMT |
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| Outline of Final Research Achievements |
In this study, we sought to identify long non-coding RNAs (lncRNAs) that may play roles in epithelial mesenchymal transition (EMT) in non-small cell lung cancer (NSCLC). We used a panel of 40 NSCLC cell lines, whose characteristics, including driver mutations, gene expression profiles, and drug sensitivities, were extensively analyzed in our previous studies. Of these cell lines, 5 epithelial-phenotype and 10 mesenchymal-phenotype cell lines were subjected to transcriptome analysis by NGS. Candidate lncRNAs that showed differential expression pattern between epithelial vs. mesenchymal cell lines were selected, and the data was further validated in the whole panel of 40 cell lines using TaqMan RT-PCR analysis. At the time of this writing, we have identified 12 candidate lncRNAs. In ongoing research, we'll further validate the results by analysis of primary tumors and in vitro functional studies of these lncRNAs.
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