| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
Carcinoma-associated fibroblasts (CAFs), frequently observed in tumor stroma, are more capable of maintaining cancer stem cells and promoting tumor growth and angiogenesis through their interactions with carcinoma cells. In this study, we performed comprehensive analysis of metalloproteinases in tumor stroma and CAFs. We found that, in stroma associated with human cancer, several metalloproteinases were significantly upregulated, whereas there was a trend towards decreased expression of tissue inhibitors of metalloproteinases (TIMPs). By analyzing CAF microarray datasets, we identified several candidates for CAF-specific metalloproteinases. CAFs also secreted exosomes containing more metalloproteinases including ADAM10 compared to normal adjacent fibroblasts. These data suggest that dysregulation of metalloproteinases in CAFs may contribute to their tumor-propagating ability in patients.
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