Elucidation of the Lipid Metabolism and Autophagic Status in the Breast Apocrine Cancer

• Project/Area Number: 25860282
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Human pathology
• Research Institution: Nihon University
• Principal Investigator: FUCHINOUE Fumi 日本大学, 医学部, 助手 (10409021)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 乳癌 / アポクリン癌 / オートファジー / 脂質代謝 / p62 / PGC1alpha

Outline of Final Research Achievements

In the recent years, metabolism-related factors have been elucidated as a cause in the increase of the cancer cells and cancerization of normal cells. In this study, three new findings have been established through investigations of lipid metabolic and autophagic status in apocrine carcinoma, which is categorized as a special type of breast carcinoma, and whether or not they relate to cell proliferation. Firstly, stimulation of lipid metabolism and impairment of autophagy are speculated in apocrine carcinomas by contrasting PGC1α and p62 expression within apocrine and non-apocrine carcinomas. Secondly, immunohistochemical analysis of p62 is useful for differential diagnosis of apocrine carcinoma and apocrine metaplasia of the breast. Finally, knockdown of p62 resulted in　a reduction of cell proliferative activity in breast cancer cell lines of the apocrine type, indicating that p62 could be a therapeutic target in apocrine carcinoma.

Research Products

• [Journal Article] Overexpression of PGC1α and accumulation of p62 in apocrine carcinoma of the breast. (2015)
  • Author(s): Fuchinoue F, Hirotani Y, Nakanishi Y, Yamaguchi H, Nishimaki H, Noda H, Tang X, Iiuzuka M, Amano S, Sugitani M, Nemoto N, Masuda S.
  • Journal Title: Patholology International Volume: 65 Issue: 1 Pages: 19-26
  • DOI: 10.1111/pin.12235
  • Peer Reviewed
• [Presentation] molecular apocrine乳癌培養細胞は、p62ノックダウンにより細胞増殖が抑制される (2015)
  • Author(s): 渕之上史
  • Organizer: 第23回日本乳癌学会学術総会
  • Place of Presentation: 東京国際フォーラム(東京都千代田区)
  • Year and Date: 2015-07-02 – 2015-07-04
• [Presentation] p62免疫組織染色をもちいた、乳腺アポクリン化生上皮とアポクリン癌との鑑別法の検討 (2015)
  • Author(s): 渕之上史
  • Organizer: 第104回日本病理学会総会
  • Place of Presentation: 名古屋国際会議場(愛知県名古屋市)
  • Year and Date: 2015-04-30 – 2015-05-02
• [Presentation] 乳腺アポクリン癌におけるPGC1α, p62の発現検討 (2014)
  • Author(s): 渕之上史
  • Organizer: 第103回日本病理学会総会
  • Place of Presentation: ANAクラウンプラザホテル広島(広島県広島市)
  • Year and Date: 2014-04-24 – 2014-04-26
• [Presentation] 乳腺アポクリン癌におけるPGC1α, p62の発現検討 (2014)
  • Author(s): 渕之上史
  • Organizer: 第103回日本病理学会総会
  • Place of Presentation: ANAクラウンプラザホテル広島