Molecular mechanism of budding formation in colon cancer metastasis.

• Project/Area Number: 25860289
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology
• Research Institution: The University of Tokyo
• Principal Investigator: Kunita Akiko 東京大学, 医学(系)研究科(研究院), 助教 (50608768)
• Co-Investigator(Renkei-kenkyūsha): MORIKAWA Teppei 東京大学, 大学院医学系研究科, 准教授 (80451772)
• Research Collaborator: TAKESHITA Kimiko 東京大学, 医学部, 学術支援職員 ; SAKUMA Kei 東京大学, 医学部, 技術専門職員
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 大腸がん / 浸潤

Outline of Final Research Achievements

In order to investigate the mechanisms of budding formation in colon cancer lymph node metastasis,colon cancer specimens which shows budding were collected, and examined the metastasis-related molecule; podoplanin expression by immunohistochemistry. Podoplanin was expressed at the budding site and active fibroblast.

Research Products

• [Journal Article] Host SHP1 phosphatase antagonizes Helicobacter pylori CagA and can be downregulated by EBV. (2016)
  • Author(s): Saju P, Kamiya MN, Hayashi T, Senda Y, Nagase L, Noda S, Matsusaka K, Funata S, Kunita A, Urabe M, Seto Y, Fukayama M, Kaneda A, Hatakeyama M.
  • Journal Title: Nat Microbiol Volume: 印刷中
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Unique clonal relationship between T-cell acute lymphoblastic leukemia and subsequent Langerhans cell histiocytosis with TCR rearrangement and NOTCH1 mutation. (2016)
  • Author(s): Takahiko Yasuda, Shinobu Tsuzuki, Masahito Kawazu, Fumihiko Hayakawa, Shinya Kojima, Toshihide Ueno, Naoto Imoto, Shinji Kohsaka, Akiko Kunita, Koichiro Doi, Toru Sakura, Toshiaki Yujiri, Eisei Kondo, Katsumichi Fujimaki, Yasunori Ueda, Yasutaka Aoyama, Shigeki Ohtake, Junko Takita, Eirin Sai, Masafumi Taniwaki, et al.
  • Journal Title: Genes Chromosomes Cancer Volume: in press Pages: 409-417
  • DOI: 10.1038/ng.3535
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Gut wall replacing type of gastrointestinal stromal tumor presenting as a perforation of the ileal diverticulum. (2015)
  • Author(s): Ikemura M, Kunita A, Miwa Y, Jimbo K, Mori K, Seto Y, Fukayama M.
  • Journal Title: Pathol. Res. Prac Volume: 211 Pages: 392-5
  • DOI: 10.1016/j.prp.2015.05.001
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The chimeric antibody chLpMab-7 targeting human podoplanin suppresses pulmonary metastasis via ADCC and CDC rather than via its neutralizing activity. (2015)
  • Author(s): Kato Y, Kunita A, Abe S, Ogasawara S, Fujii Y, Oki H, Fukayama M, Nishioka Y, Kaneko MK.
  • Journal Title: Oncotarget Volume: 6(34) Pages: 36003-18
  • DOI: 10.18632/oncotarget.5339
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Update on Epstein-Barr virus and gastric cancer (review) (2015)
  • Author(s): Shinozaki-Ushiku A, Kunita A, Fukayama M.
  • Journal Title: Int J Oncol. Volume: 46 Pages: 1421-1434
  • DOI: 10.3892/ijo.2015.2856
  • Peer Reviewed
• [Journal Article] Profiling of Virus-Encoded MicroRNAs in Epstein-Barr Virus-Associated Gastric Carcinoma and Their Roles in Gastric Carcinogenesis (2015)
  • Author(s): Shinozaki-Ushiku A, Kunita A, Isogai M, Hibiya T, Ushiku T, Takada K, and Fukayama M
  • Journal Title: J Virol. Volume: 89 Pages: 5581-5591
  • DOI: 10.1128/jvi.03639-14
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Hunner-Type (Classic) Interstitial Cystitis: A Distinct Inflammatory Disorder Characterized (2015)
  • Author(s): Maeda D, Akiyama Y, Morikawa T, Kunita A, Ota Y, Katoh H, Niimi A, Nomiya A, Ishikawa S, Goto A, Igawa Y, Fukayama M, Homma Y.
  • Journal Title: Plos One. Volume: 10 Pages: 1-16
  • DOI: 10.1371/journal.pone.0143316
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The Niche Component Periostin Is Produced by Cancer-Associated Fibroblasts, Supporting Growth of Gastric Cancer through ERK Activation. (2014)
  • Author(s): Kikuchi Y, Kunita A, Iwata C, Komura D, Nishiyama T, Shimazu K, Takeshita K, Shibahara J, Kii I, Morishita Y, Yashiro M, Hirakawa K, Miyazono K, Kudo A, Fukayama M, Kashima TG.
  • Journal Title: American Journal of Pathology Volume: 184 Pages: 859-870
  • DOI: 10.1016/j.ajpath.2013.11.012
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] HER2 protein overexpression and gene amplification in upper urinary tract urothelial carcinoma-an analysis of 171 patients (2014)
  • Author(s): asaki, Y, Sasaki, T, Kawai, T, Morikawa, T, Matsusaka, K, Kunita, A., Kume, H, Aoki, I, Homma, Y, and Fukayama, M.
  • Journal Title: International Journal of Clinical and Experimental Pathology Volume: 7 Pages: 699-708
  • Peer Reviewed
• [Journal Article] VEGF-Mediated Angiogenesis Links EMT-Induced Cancer Stemness to Tumor Initiation. (2014)
  • Author(s): Fantozzi, A, Gruber, D. C, Pisarsky, L, Heck, C, Kunita, A, Yilmaz, M, Meyer-Schaller, N, Cornille, K, Hopfer, U, Bentires-Alj, M, and Christofori, G.
  • Journal Title: Cancer Research Volume: 74 Pages: 1566-1575
  • DOI: 10.1158/0008-5472.can-13-1641
  • Peer Reviewed
• [Journal Article] Enrichment of stem-like cell population comprises transformation ability of Epstein-Barr virus latent membrane protein 2A for non-transformed cells. (2014)
  • Author(s): Tanaka M, Suzuki HI, Shibahara J, Kunita A, Isagawa T, Yoshimi A, Kurokawa M, Miyazono K, Aburatani H, Ishikawa S, Fukayama M.
  • Journal Title: Virus Research Volume: 33(19) Pages: 1-10
  • DOI: 10.1038/onc.2013.204
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 新規抗ポドプラニンキメラ抗体による抗腫瘍効果の検討 (2015)
  • Author(s): 国田朱子、 小笠原諭、金子美華、加藤幸成
  • Organizer: 第38回日本分子生物学会年会・第88回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] マウス胃癌モデルを用いたLMP2Aの機能解析 (2015)
  • Author(s): 国田朱子、市村香代子、沼倉里枝、日比谷孝志、坂谷貴司、大島正伸 、深山正久
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2015-10-08
• [Presentation] 新規抗ポドプラニンキメラ抗体による転移抑制効果の検討 (2015)
  • Author(s): 国田 朱子、加藤 幸成、大木 弘治、西岡 安彦、深山 正久
  • Organizer: 第２４回日本がん転移学会学術集会総会
  • Place of Presentation: シティプラザ大阪（大阪府中央区）
  • Year and Date: 2015-07-23
• [Presentation] Epstein-Barr Virus-encoded MicroRNAs in Gastric Carcinoma (2015)
  • Author(s): 国田朱子、牛久 綾、 阿部浩幸、 日向宗利、深山正久
  • Organizer: 第5回新学術領域発がんスパイラル国際シンポジウム
  • Place of Presentation: 神戸
  • Year and Date: 2015-02-26 – 2015-02-27
• [Presentation] Identification of Interleukin 13 Receptor alpha 2 (IL13Rα2) as a novel marker of human melanoma (2015)
  • Author(s): Mao Komai, Akiko Kunita, Teppei Morikawa, Hayato Okamoto1, Moegi Sato1, Hiroaki Uchida1, Yasuhiro Yoshimatsu1, Takeshi Fukuhara, Masashi Fukayama, Tetsuro Watabe
  • Organizer: Joint International Symposium on TGF-B Family and Cancer
  • Place of Presentation: 筑波
  • Year and Date: 2015-01-12 – 2015-01-13
• [Presentation] Leptin receptor expression and loss of SOCS3 in Epstein-Barr virus –associated gastric carcinoma (EBVaGC). (2014)
  • Author(s): 国田朱子，日野るみ、阿部浩幸、深山正久
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] EBウイルス関連胃癌におけるウイルス由来マイクロRNAの特性解析 (2014)
  • Author(s): 国田朱子，阿部浩幸、日向宗利、牛久綾、深山正久
  • Organizer: 第6回日本RNAi研究会
  • Place of Presentation: 広島
  • Year and Date: 2014-08-28 – 2014-08-30
• [Presentation] 肺腺癌腫瘍間質におけるmiR-21の発現 (2014)
  • Author(s): 国田朱子、森田茂樹、深山正久
  • Organizer: 第33回分子病理学研究会宮城蔵王シンポジウム
  • Place of Presentation: 宮城蔵王
  • Year and Date: 2014-07-25 – 2014-07-26
• [Presentation] Expression of miR-21 in cancer associated fibroblasts in lung adenocarcinoma (2014)
  • Author(s): Akiko Kunita, Shigeki Morita, Masashi Fukayama
  • Organizer: AACR Annual Meeting 2014
  • Place of Presentation: 米国 サンディエゴ
  • Year and Date: 2014-04-05 – 2014-04-09
• [Presentation] 腫瘍浸潤先端部におけるポドプラニンの発現解析 (2013)
  • Author(s): 国田朱子，深山正久
  • Organizer: がん若手ワークショップ
  • Place of Presentation: 蓼科
• [Presentation] Podoplanin expression at the tumor invasive front (2013)
  • Author(s): Akiko Kunita, Masashi Fukayama
  • Organizer: AACR International Conference on Frontiers in Basic Cancer Research
  • Place of Presentation: 米国、ナショナルハーバー
• [Presentation] 腫瘍浸潤先端部におけるpodoplaninの発現 (2013)
  • Author(s): 国田朱子，深山正久
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜