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New Cancer Treatment Model Using Anti-MCM2 Intrabody

Research Project

Project/Area Number 25860290
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Experimental pathology
Research InstitutionTokyo Medical and Dental University

Principal Investigator

ABE Shinya  東京医科歯科大学, 医歯(薬)学総合研究科, 助教 (70596725)

Co-Investigator(Renkei-kenkyūsha) KITAGAWA Masanobu  東京医科歯科大学, 大学院医歯学総合研究科, 教授 (10177834)
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
KeywordsMCM2 / アポトーシス / 治療モデル / 細胞内抗体 / ファージディスプレイ / DNA damage
Outline of Final Research Achievements

In a previous study, we showed that Friend leukemia virus (FLV) envelope protein gp70 bound MCM2, impaired its nuclear translocation, and enhanced DNA-damage-induced apoptosis in hematopoietic cells when the cells expressed high levels of MCM2.
Here, we aimed to create a new cancer treatment model by interrupting the nuclear transition of MCM2 using the anti-MCM2 intrabody instead of gp70. We first conducted separation of anti-MCM2 antibody by phage display method, and were successful to separate specific antibody against MCM2. We, furthermore, confirmed that, in vitro and in vivo, this antibody connects with MCM2 in the cell and fortifies apoptosis caused by the anti-cancer drug. It is thought that, by furthering the study, this treatment by anti-MCM2 intrabody would be a model for new tumor treatments.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (11 results)

All 2014 2013

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (7 results)

  • [Journal Article] Screening with a novel cell-based assay for TAZ activators identifies a compound that enhances myogenesis in C2C12 cells and facilitates muscle repair in the muscle injury model.2014

    • Author(s)
      Yang Z, Nakagawa K, Sarkar A, Maruyama J, Iwasa H, Bao Y, Ishigami-Yuasa M, Ito S, Kagechika H, Hata S, Nishina H, Abe S, Kitagawa M, Hata Y
    • Journal Title

      Mol. Cell Biol.

      Volume: -

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Over-Expression of Cancerous Inhibitor of PP2A (CIP2A) in Bone Marrow Cells from Patients with a Group of High-Risk Myelodysplastic Syndromes.2014

    • Author(s)
      Li N, Abe S, Kurata M, Abe-Suzuki S, Onishi I, Kirimura S, Murayama T, Hidaka M, Kawano F, Kitagawa M
    • Journal Title

      Pathol Oncol Res.

      Volume: 20 Issue: 2 Pages: 399-407

    • DOI

      10.1007/s12253-013-9709-y

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Atrial natriuretic peptide attenuates kidney-lung crosstalk in kidney injury2014

    • Author(s)
      Tulafu M, Mitaka C, Hnin Si MK, Abe S, Kitagawa M, Ikeda S, Eishi Y, Kurata S, Tomita M
    • Journal Title

      J Surg Res

      Volume: 186 Issue: 1 Pages: 217-225

    • DOI

      10.1016/j.jss.2013.07.033

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Inhibition of poly (adenosine diphosphate-ribose) polymerase attenuates lung-kidney crosstalk induced by intratracheal lipopolysaccharide instillation in rats2013

    • Author(s)
      Si MK, Mitaka C, Tulafu M, Abe S, Kitagawa M, Ikeda S, Eishi Y, Kurata S, Tomita M.
    • Journal Title

      Respiratory Research

      Volume: 14 Issue: 1 Pages: 126-133

    • DOI

      10.1186/1465-9921-14-126

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] A novel model of cancer therapy by enhancing DNA-damage-induced apoptosis.2014

    • Author(s)
      阿部晋也、山本浩平、阿部志保、桐村進、北川昌伸
    • Organizer
      第73回日本癌学会総会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Apoptotic enhancement by MCM22014

    • Author(s)
      北川昌伸、阿部晋也、阿部志保、倉田盛人、山本浩平、大西威一郎、桐村進、木原淳、遠藤祐子
    • Organizer
      第73回日本癌学会総会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] MCM2の作用を用いた新規腫瘍治療モデル2014

    • Author(s)
      阿部晋也、山本浩平、阿部志保、大西威一郎、桐村進、北川昌伸
    • Organizer
      第103回日本病理学会
    • Place of Presentation
      広島国際会議場(広島県・広島市)
    • Year and Date
      2014-04-24 – 2014-04-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] MCM2によるアポトーシス増強機構2014

    • Author(s)
      北川昌伸、阿部晋也、倉田盛人、山本浩平、阿部志保、大西威一郎、桐村進
    • Organizer
      第103回日本病理学会
    • Place of Presentation
      広島国際会議場(広島県・広島市)
    • Year and Date
      2014-04-24 – 2014-04-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] DNA損傷誘発アポトーシス増強におけるMCM2の役割2013

    • Author(s)
      阿部晋也、阿部志保、山本浩平、北川昌伸
    • Organizer
      第36回日本分子生物学会
    • Place of Presentation
      神戸
    • Related Report
      2013 Research-status Report
  • [Presentation] CM2 bound with retroviral gp70 is localized to cytoplasm and enhanced DNA-damage-induced apoptosis.2013

    • Author(s)
      阿部晋也、倉田盛人、阿部志保、大西威一郎、小川弘恵、北川昌伸
    • Organizer
      第72回日本癌学会総会
    • Place of Presentation
      横浜
    • Related Report
      2013 Research-status Report
  • [Presentation] DNA損傷誘発アポトーシス増強によるin vivo腫瘍治療モデル2013

    • Author(s)
      阿部晋也、倉田盛人、鈴木志保、小川弘恵、北川昌伸
    • Organizer
      第102回日本病理学会総会
    • Place of Presentation
      札幌
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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