Functional analyses of Twist-1 in malignant mesothelioma using animal carcinogensis model.

• Project/Area Number: 25860292
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology
• Research Institution: Nagoya University
• Principal Investigator: OKAZAKI YASUMASA 名古屋大学, 医学(系)研究科(研究院), 講師 (30403489)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 中皮腫 / Twist-1 / 鉄 / 酸化ストレス / microRNA / 転写因子 / 腹膜中皮腫

Outline of Final Research Achievements

With the aid of this grant, Twist-1 is also detected in carbon nanotubes (CNT)-induced SM. The expression of Twist-1 was not significantly affected the life span in asbestos and CNT-induced rat MM. The expression of MDM2, which is induced by Twist-1 and works to degradate p53, and Ki67, which is a marker for cellular proliferation, were associated with Twist-1 expression. These results suggest that Twist-1 has potent proliferative effect on MM in vivo. The examination for the expression of transcriptional factors, which are reported to be a candidate for the induction of Twist-1, disclosed the relation between Twist-1 and HIF1alpha expression in CNT-induced rat MM.

Research Products

• [Journal Article] Direct exposure of non-equilibrium atmospheric pressure plasma confers simultaneous oxidative and ultraviolet modifications in biomolecules (2014)
  • Author(s): Yasumasa Okazaki, Yue Wang, Hiromasa Tanaka, Masaaki Mizuno, Kae Nakamura, Hiroaki Kajiyama, Hiroyuki Kano, Koji Uchida, Fumitaka Kikkawa, Masaru Hori, Shinya Toyokuni
  • Journal Title: J Clin Biochem Nutr. Volume: 55 Issue: 3 Pages: 207-15
  • DOI: 10.3164/jcbn.14-40
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Rat model demonstrates a high risk of tremolite but a low risk of anthophyllite for mesothelial carcinogenesis (2014)
  • Author(s): Aierken D, Okazaki Y, Chew SH, Sakai A, Wang Y, Nagai H, Misawa N, Kohyama N, Toyokuni S
  • Journal Title: Nagoya J Med Sci Volume: 76(1-2) Pages: 149-160
  • Peer Reviewed / Open Access
• [Journal Article] Plasma Medical Science for Cancer Therapy. ~toward cancer therapy using non-thermal atmospheric pressure plasma~ (2014)
  • Author(s): Hiromasa Tanaka, Masaaki Mizuno, Kenji Ishikawa, Keigo Takeda, Kae Nakamura, Fumi Utsumi, Hiroaki Kajiyama, Hiroyuki Kano, Yasumasa Okazaki, Shinnya Toyokuni,Shoichi Maruyama, Fumitaka Kikkawa, Masaru Hori
  • Journal Title: IEEE Transactions on Plasma Science Volume: 42 Pages: 3760-3763
  • DOI: 10.1109/tps.2014.2353659
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Cancer-promoting role of adipocytes in asbestos-induced mesothelial carcinogenesis through dysregulated adipocytokine production. (2014)
  • Author(s): Chew SH, Okazaki Y, Nagai H, Misawa N, Akatsuka S, Yamashita K, Jiang L, Yamashita Y, Noguchi M, Hosoda K, Sekido Y, Takahashi T, Toyokuni S
  • Journal Title: Cancer-promoting role of adipocytes in asbestos-induced mesothelial carcinogenesis through dysregulated adipocytokine production Volume: 35 Pages: 164-172
  • DOI: 10.1093/carcin/bgt267
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Deferasirox induces mesenchymal-epithelial transition in crocidolite-induced mesothelial carcinogenesis in rats (2013)
  • Author(s): Nagai H, Okazaki Y, Chew SH, Misawa N, Yasui H and Toyokuni S.
  • Journal Title: Cance Prev Res Volume: 6 Pages: 1222-1230
  • DOI: 10.1158/1940-6207.capr-13-0244
  • Peer Reviewed
• [Journal Article] Intraperitoneal administration of tangled multiwalled carbon nanotubes of 15 nm in diameter does not induce mesothelial carcinogenesis in rats (2013)
  • Author(s): H.Nagai
  • Journal Title: Pathol Int Volume: 63 Pages: 457-462
  • DOI: 10.1111/pin.12093
  • Peer Reviewed
• [Presentation] NON-EQUILIBRIUM ATMOSPHERIC PRESSURE PLASMA (NEAPP) GENERATES OXIDATIVE INJURY (2015)
  • Author(s): Okazaki Y, Wang Y, Tanaka H, Mizuno M, Nakamura K, Kajiyama H, Kano H, Uchida K, Kikkawa F, Hori M,Toyokuni S
  • Organizer: IWPCT-2 (International Workshop on Plasma for Cancer Treatment-2)
  • Place of Presentation: 名古屋大学（愛知県名古屋市）
  • Year and Date: 2015-03-16 – 2015-03-17
• [Presentation] Non-equilibrium atmospheric pressure plasma (NEAPP) generates oxidative injury (2014)
  • Author(s): Yasumasa Okazaki, Yue Wang, Hiromasa Tanaka, Masaaki Mizuno, Kae Nakamura, Hiroaki Kajiyama, Hiroyuki Kano, Fumitaka Kikkawa, Masaru Hori, Shinya Toyokuni
  • Organizer: Society for Free Radical Research International 2014
  • Place of Presentation: 京都国際会議場（京都市）
• [Presentation] Non-equilibrium atmospheric pressure plasma (NEAPP) generates oxidative injury (2014)
  • Author(s): Okazaki Y, Wang Y, Tanaka H, Mizuno M, Nakamura K, Kajiyama H, Kano H, Kikkawa F, Hori M,Toyokuni S
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 非平衡大気圧プラズマは酸化ストレスを生じる (2014)
  • Author(s): 岡崎泰昌, 王越, 田中宏昌, 水野正明, 中村香江, 梶山広明, 加納浩之, 内田浩二, 吉川史隆, 堀勝, 豊國伸哉
  • Organizer: 第38回日本鉄バイオサイエンス学会学術集会
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2014-09-06 – 2014-09-07
• [Presentation] 非平衡大気圧プラズマは酸化ストレスを生じる (2014)
  • Author(s): 岡崎泰昌, 王越, 田中宏昌, 水野正明, 中村香江, 梶山広明, 加納浩之, 内田浩二, 吉川史隆, 堀勝, 豊國伸哉
  • Organizer: 第67回日本酸化ストレス学会学術集会
  • Place of Presentation: 同志社大学（京都府京都市）
  • Year and Date: 2014-09-04 – 2014-09-05
• [Presentation] 非平衡大気圧プラズマは酸化ストレスを生じる (2014)
  • Author(s): 岡崎泰昌, 王越, 田中宏昌, 水野正明, 中村香江, 梶山広明, 加納浩之, 内田浩二, 吉川史隆, 堀勝, 豊國伸哉
  • Organizer: 第11回病理学会カンファレンス
  • Place of Presentation: 六甲山ホテル（兵庫県神戸市）
  • Year and Date: 2014-08-01 – 2014-08-02
• [Presentation] Non-equilibrium atmospheric pressure plasma (NEAPP) generates oxidative injury (2014)
  • Author(s): Okazaki Y, Wang Y, Tanaka H, Mizuno M, Nakamura K, Kajiyama H, Kano H, Kikkawa F, Hori M,Toyokuni S
  • Organizer: ICPM-5 (International Conference on Plasma Medicine-5)
  • Place of Presentation: 奈良県立公会堂（奈良県奈良市）
  • Year and Date: 2014-05-18 – 2014-05-23
• [Presentation] DAP (DMT1 ASSOCIATED PROTEIN) REGULATES CELL PROLIFERATION THROUGH THE REGULATION OF DMT1 (IRE) (2013)
  • Author(s): Yasumasa Okazaki, Hirotaka Nagai, Satomi Funahashi, Shan-Hwu Chew, Mary Yeh, Kwo-yih Yeh, Jonathan Glass, Shinya Toyokuni
  • Organizer: Biennial meeting of Society for Free Radical Research-Asia
  • Place of Presentation: 台北（台湾）
• [Presentation] CD146、IMP3の過剰発現はアスベスト誘発ラット悪性腹膜中皮腫の生存期間を短縮する (2013)
  • Author(s): 岡崎泰昌、永井裕崇、周珊瑚、蒋麗、舟橋諭美、辻村亨、豊國伸哉
  • Organizer: 第37回日本鉄バイオサイエンス学会学術集会
  • Place of Presentation: JA共済ビル（東京都）
• [Presentation] CD146、IMP3の過剰発現はアスベスト誘発ラット悪性腹膜中皮腫の生存期間を短縮する (2013)
  • Author(s): 岡崎泰昌、永井裕崇、周珊瑚、蒋麗、舟橋諭美、辻村亨、豊國伸哉
  • Organizer: 第66回日本酸化ストレス学会
  • Place of Presentation: ウインク愛知（名古屋市）
• [Presentation] 実験病理学分野における若手研究者のアメリカ留学の一例 (2013)
  • Author(s): 岡崎泰昌
  • Organizer: 第102回日本病理学会総会
  • Place of Presentation: ロイトン札幌（札幌市）
• [Book] ロビンス 基礎病理学 原書9版 (2014)
  • Author(s): （分担翻訳）岡崎泰昌
  • Total Pages: 1087
  • Publisher: 丸善出版、エルゼビア ジャパン
• [Book] 改訂第二版 酸化ストレスの医学 (2014)
  • Author(s): （分担執筆）岡崎泰昌, et al.
  • Total Pages: 444
  • Publisher: 診断と治療社
• [Book] 活性酸素の本当の姿 (2013)
  • Author(s): 藤原 範子 (著), 大河原知水 (著), 木崎 節子 (著), 李 昌一 (著), 住本 英樹 (著), 赤池 孝章 (著), 藤井 重元 (著), 岡崎 泰昌 (著), 豊國 伸哉 (著), 内藤 裕二 (著), 大野 秀樹 (著), 鈴木敬一郎 (編集)
  • Total Pages: 176
  • Publisher: ナップ