Analyses of mechanisms of regulation of cell fusion and formation of tumor-associated multinucleated giant cells
Project/Area Number |
25860293
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Experimental pathology
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Research Institution | Nagoya University |
Principal Investigator |
Mii Shinji 名古屋大学, 医学(系)研究科(研究院), 助教 (80646505)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | CD109 / 破骨細胞 / 間葉系間質細胞 / 骨肉腫 / 骨芽細胞 |
Outline of Final Research Achievements |
In this study, immunohistochemical analysis revealed CD109 expression in human and murine osteoclasts in bones and osteoclast-like multinucleated giant cells in human osteosarcoma and giant cell tumor. In addition, we performed co-culture experiments using primary osteoblasts and pre-osteoclasts isolated from either wild-type or CD109-deficient mice. In this in vitro co-culture experiments, CD109-deficient pre-osteoclasts failed to multinucleate by cell fusion. These findings suggest that CD109 plays a role in cell fusion of osteoclasts and in formation of osteoclast-like multinucleated giant cells.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] Identification of Meflin as a Potential Marker for Mesenchymal Stromal Cells2016
Author(s)
Maeda K, Enomoto A, Hara A, Asai N, Kobayashi T, Horinouchi A, Maruyama S, Ishikawa Y, Nishiyama T, Kiyoi H, Kato T, Ando K, Weng L, Mii S, Asai M, Mizutani Y, Watanabe O, Hirooka Y, Goto H, Takahashi M
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Journal Title
Sci Rep
Volume: 6
Issue: 1
Pages: 22288-22288
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] CD109 attenuates TGF-β1 signaling and enhances EGF signaling in SK-MG-1 human glioblastoma cells.2015
Author(s)
Jing-Min Zhang, Yoshiki Murakumo, Sumitaka Hagiwara, Ping Jiang, Shinji Mii, Emir Kalyoncu, Shoji Saito, Chikage Suzuki, Yasutaka Sakurai, Yoshiko Numata, Toshimichi Yamamoto, Masahide Takahashi
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Journal Title
Biochemical and Biophysical Research Communications
Volume: 459
Issue: 2
Pages: 252-258
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Akt-girdin signaling in cancer-associated fibroblasts contributes to tumor progression2015
Author(s)
Yumiko Yamamura, Naoya Asai, Atsushi Enomoto, Takuya Kato, Shinji Mii, Yuji Kondo, Kaori Ushida, Kaoru Niimi, Nobuyuki Tsunoda, Masato Nagino, Shu Ichihara, Koichi Furukawa, Kengo Maeda, Toyoaki Murohara, Masahide Takahashi
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Journal Title
Cancer Research
Volume: 75
Issue: 5
Pages: 813-823
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Suppression of REV7 enhances cisplatin sensitivity in ovarian clear cell carcinoma cells.2014
Author(s)
Niimi K, Murakumo Y, Watanabe N, Kato T, Mii S, Enomoto A, Asai M, Asai N, Yamamoto E, Kajiyama H, Shibata K, Kikkawa F, Takahashi M.
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Journal Title
Cancer Sci.
Volume: 105
Issue: 5
Pages: 545-52
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] TRIM27/MRTF-B-Dependent Integrin β1 Expression Defines Leading Cells in Cancer Cell Collectives.2014
Author(s)
Takuya Kato, Atsushi Enomoto, Takashi Watanabe, Hisashi Haga, Sumire Ishida, Yuji Kondo, Koichi Furukawa, Takeshi Urano, Shinji Mii, Liang Weng, Maki Ishida-Takagishi, Masato Asai, Naoya Asai, Kozo Kaibuchi, Yoshiki Murakumo, Masahide Takahashi.
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Journal Title
Cell reports.
Volume: 7
Issue: 4
Pages: 1156-1167
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Detection of a Soluble Form of CD109 in Serum of CD109 Transgenic and Tumor Xenografted Mice.2014
Author(s)
Sakakura H, Murakumo Y, Mii S, Hagiwara S, Kato T, Asai M, Hoshino A, Yamamoto N, Sobue S, Ichihara M, Ueda M, Takahashi M.
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Journal Title
PLoS One
Volume: 9
Issue: 1
Pages: e83385-e83385
DOI
Related Report
Peer Reviewed
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[Journal Article] The REV7 subunit of DNA polymerase ζ is essential for primordial germ cell maintenance in the mouse.2013
Author(s)
Watanabe, N., Mii, S., Asai, N., Asai, M., Niimi, K., Ushida, K., Kato, T., Enomoto, A., Ishii, H., Takahashi, M., Murakumo, Y.
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Journal Title
J. Biol. Chem.
Volume: 288
Issue: 15
Pages: 10459-10471
DOI
Related Report
Peer Reviewed
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