Transcriptome analysis of glucose metabolism disorder using a zebrafish diet-induced obesity model

• Project/Area Number: 25860294
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology
• Research Institution: Mie University
• Principal Investigator: ZANG Liqing 三重大学, 医学部, 技術員 (10437105)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 糖代謝異常 / ゼブラフィッシュ / 肥満モデル / 遺伝子発現抑制 / インスリン / 糖尿病治療薬 / RNA-Seq

Outline of Final Research Achievements

Glucose metabolism disorders, such as diabetes mellitus, are pathological conditions in which the blood glucose cannot be maintained within the normal range. The pathogenesis of these conditions are either relatively low insulin production or insulin resistance or both, but there are little understanding of the mechanisms at present. In this study, we performed comparative transcriptome analysis of liver and pancreas tissue from diet-induced obese zebrafish using next-generation sequencer. Bioinformatics analysis revealed that obese zebrafish and human share common pathophysiological pathways related to glucose metabolism, and several new treatment target gene candidates were identified. In addition, we demonstrated that this diet-induced obesity model is utility as an animal model for human glucose metabolism disorders by gene knockdown experiments and diabetes therapeutic drugs treatment.

Research Products

• [Journal Article] Repeated blood collection for blood tests in adult zebrafish (2015)
  • Author(s): Zang L, Shimada Y, Nishimura Y, Tanaka T, Nishimura N.
  • Journal Title: Journal of Visualized Experiments Volume: 未定
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Effects of Yuzu (Citrus junos Siebold ex Tanaka) peel on the diet-induced obesity in a zebrafish model (2014)
  • Author(s): Liqing Zang, Yasuhito Shimada, Jumpei Kawajiria, Toshio Tanaka, Norihiro Nishimura
  • Journal Title: Journal of Functional Foods Volume: 10 Pages: 499-510
  • DOI: 10.1016/j.jff.2014.08.002
  • Peer Reviewed / Open Access
• [Journal Article] Eriocitrin ameliorates diet-induced hepatic steatosis with activation of mitochondrial biogenesis (2014)
  • Author(s): Masanori Hiramitsu, Yasuhito Shimada,Junya Kuroyanagi,Takashi Inoue,Takao Katagiri,Liqing Zang,Yuhei Nishimura,Norihiro Nishimura & Toshio Tanaka
  • Journal Title: Scientific Reports Volume: 4 Issue: 1 Pages: 3708-3708
  • DOI: 10.1038/srep03708
  • Peer Reviewed
• [Journal Article] A Novel, Reliable Method for Repeated Blood Collection from Aquarium Fish. (2013)
  • Author(s): Zang L, Shimada Y, Nishimura Y, Tanaka T, Nishimura N.
  • Journal Title: Zebrafish Volume: 10 Issue: 3 Pages: 425-432
  • DOI: 10.1089/zeb.2012.0862
  • Peer Reviewed
• [Journal Article] Fluorescent-Based Methods for Gene Knockdown and Functional Cardiac Imaging in Zebrafish. (2013)
  • Author(s): Umemoto N, Nishimura Y, Shimada Y, Yamanaka Y, Kishi S, Ito S, Okamori K, Nakamura Y, Kuroyanagi J, Zhang Z, Zang L, Wang Z, Nishimura N, Tanaka T.
  • Journal Title: Molecular Biotechnology Volume: 55 Issue: 2 Pages: 131-142
  • DOI: 10.1007/s12033-013-9664-6
  • Peer Reviewed
• [Patent(Industrial Property Rights)] 脂質の生体内沈着抑制剤、飼料および飼育方法 (2014)
  • Inventor(s): 伊藤克、籠谷和弘、臧黎清、西村訓弘
  • Industrial Property Rights Holder: 伊藤克、籠谷和弘、臧黎清、西村訓弘
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-05-22