Role of long non-coding RNA in regulation of inflammatory cytokine expression
Project/Area Number |
25860307
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Experimental pathology
|
Research Institution | Tokyo Medical and Dental University |
Principal Investigator |
CHIBA Tomoki 東京医科歯科大学, 医歯(薬)学総合研究科, 特任研究員 (00645830)
|
Co-Investigator(Renkei-kenkyūsha) |
ASAHARA Hiroshi 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (70294460)
KATO Tomomi 東京医科歯科大学, 大学院医歯学総合研究科, 技術補佐員
|
Project Period (FY) |
2013-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | 非コードRNA / 炎症性サイトカイン |
Outline of Final Research Achievements |
Recent advantages of transcriptome analyses revealed that non-coding RNAs, especially long non-coding (lnc) RNAs, are transcribed across genome and involved in various aspects of biological processes. I identified novel lncRNA that is essential for inflammatory cytokine expression. IL-6 and GM-CSF expression was severely reduced by lncRNA silencing upon LPS stimulation. LncRNA was mainly localized in cytoplasm and did not influence NF-kappaB activation. Further, to understand physiological roles, lncRNA transgenic and knockout mice were successfully generated by transposon and CRISPR/Cas9 system, respectively.
|
Report
(3 results)
Research Products
(2 results)
-
[Journal Article] TALEN-Mediated Gene Disruption on Y Chromosome Reveals Critical Role of EIF2S3Y in Mouse Spermatogenesis2015
Author(s)
Matsubara Yohei, Kato Tomoko, Kashimada Kenichi, Tanaka Hiromitsu, Zhi Zhou, Ichinose Shizuko, Mizutani Shuki, Morio Tomohiro, Chiba Tomoki, Ito Yoshiaki, Saga Yumiko, Takada Shuji, and Asahara Hiroshi
-
Journal Title
Stem Cells and Development
Volume: 24
Issue: 10
Pages: 1164-1170
DOI
Related Report
Peer Reviewed / Open Access
-