A novel dsRNA mycovirus reduces pathogenicity of Aspergillus fumigatus in a mouse infection model.
| Project/Area Number |
25860314
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | Chiba University |
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Principal Investigator |
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| Research Collaborator |
URAYAMA Syun-ishi 東京農工大学
YAHARA Misa 千葉大学, 大学院生
SHISHIDO Erika 千葉大学, 大学院生
HIBIYA Yuko 国立がん研究センター
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | マイコウイルス / Aspergillus / A.fumigatus / アスペルギルス |
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| Outline of Final Research Achievements |
In a screen of 44 clinical and environmental isolates of A. fumigatus for mycoviruses, four of the isolates were found to contain distinct groups of dsRNA segments. By using next-generation sequencer (illimina, Miseq), we have sequenced the dsRNA mycovitus genomes of these mycoviruses which are respectively members of the Chrysovirus genus, the Partitivirus genus, and 2 of novel mycoviruses. In this investigation, isogenic lines of virus-free and virus-infected A. fumigatus for each mycovirus infections have been compared with respect to phenotypic differences and effects on host fitness. Virulence assays in mouse model implicated that a novel mycovirus influence for normal pathogenesis. In addition, we also will report that mycovirus have influence on A. fumigatus characterization, such as, number of spores, mycelia morphology, adherence of A. fumigatus resting conidia to host pulmonary epithelial cells, and tolerance for macrophage phagocytosis.
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Report
(5 results)
Research Products
(11 results)
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[Journal Article] Discrepancy between Hepatitis C Virus Genotypes and NS4-Based Serotypes: Association with Their Subgenomic Sequences.2017
Author(s)
Nan Nwe Win, Shingo Nakamoto, Tatsuo Kanda, Hiroki Takahashi, Azusa Takahashi-Nakaguchi, Shin Yasui, Masato Nakamura, Shuang Wu, Fumio Imazeki, Shigeru Mikami, Osamu Yokosuka, Tohru Gonoi, Hiroshi Shirasawa
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Journal Title
International Journal of Molecular Sciences
Volume: 18(1)
Issue: 1
Pages: 172-172
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The CgHaa1-Regulon Mediates Response and Tolerance to Acetic Acid Stress in the Human Pathogen Candida glabrata.2016
Author(s)
Ruben T Bernardo, Diana V Cunha, Can Wang, Leonel Pereira, Sand 243;nia Silva, Sara B Salazar, Markus S Schr246;der, Michiyo Okamoto, Azusa Takahashi-Nakaguchi, Hiroji Chibana, Toshihiro Aoyama, Isabel S225;-Correia, Joana Azeredo, Geraldine Butler, Nuno Pereira Mira
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Journal Title
Genomes Genetics
Volume: 7(1)
Issue: 1
Pages: 1-18
DOI
Related Report
Peer Reviewed
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