Study for structure and function of botulinum hemagglutinin

• Project/Area Number: 25860320
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including mycology)
• Research Institution: Tokyo University of Agriculture (2014-2015); Okayama University (2013)
• Principal Investigator: Suzuki Tomonori 東京農業大学, 応用生物科学部, 助教 (90453836)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: Clostridium botulinum / 毒素複合体 / ボツリヌス毒素 / 血球凝集素 / タンパク質間相互作用 / タンパク質複合体 / 立体構造 / 立体構造予測

Outline of Final Research Achievements

The large toxin complex (L-TC) produced by Clostridium botulinum is formed from neurotoxin and four non-toxic proteins. In particular, HA components appear to play a role in the transport of the TC across the intestinal epithelium. In this study, the binding regions between HA subcomponents were examined.

Research Products

• [Journal Article] Genomics of Clostridium botulinum group III strains (2015)
  • Author(s): Sakaguchi, Y., Suzuki, T., Yamamoto, Y., Nishikawa, A., Oguma, K.
  • Journal Title: Res. Microbiol. Volume: 166 Issue: 4 Pages: 318-325
  • DOI: 10.1016/j.resmic.2014.07.016
  • Peer Reviewed / Open Access
• [Journal Article] Identification of the interaction region between hemagglutinin components of the botulinum toxin complex (2014)
  • Author(s): Suzuki T, Miyashita S, Hayashi S, Miyata K, Inui K, Kondo Y, Miyazaki S, Ohyama T, Niwa K, Watanabe T, Sagane Y
  • Journal Title: International Journal of Biological Macromolecules Volume: 65 Pages: 284-288
  • DOI: 10.1016/j.ijbiomac.2014.01.052
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Sugar-induced conformational change found in the HA-33/HA-17 trimer of the botulinum toxin complex (2013)
  • Author(s): Yoshimasa Sagane, Shintaro Hayash, Takashi Matsumoto, Shin-ichro Miyashita, Ken Inui, Keita Miyata, Shunsuke Yajima, Tomonori Suzuki, Kimiko Hasegawa, Akihito Yamano, Atushi Nishikawa, Tohru Ohyama, Toshihiro Watanabe, Koichi Niwa
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 438 Issue: 3 Pages: 483-487
  • DOI: 10.1016/j.bbrc.2013.07.112
  • Peer Reviewed
• [Journal Article] Botulinum Toxin Complex Increases Paracellular Permeability in Intestinal Epithelial Cells via Activation of p38 Mitogen-Activated Protein Kinase (2013)
  • Author(s): Miyashita SI, Sagane Y, Inui K, Hayashi S, Miyata K, Suzuki T, Ohyama T, Watanabe T, Niwa K.
  • Journal Title: The Journal of Veterinary Medical Science Volume: 75 Pages: 1637-1642
  • NAID: 130003362264
  • Peer Reviewed
• [Journal Article] Random phage display based screening of peptides that bind to botulinum neurotoxin binding protein, nontoxic nonhemagglutinin. (2013)
  • Author(s): Sagane Y, Miyata K, Kurihara S, Yoneyama T, Inui K, Miyashita S, Hayashi S, Suzuki T, Niwa K,
  • Journal Title: Current Microbiology Volume: (Online) Issue: 2 Pages: 188-192
  • DOI: 10.1007/s00284-013-0355-0
  • Peer Reviewed
• [Journal Article] Phospholipase C produced by Clostridium botulinum types C and D: comparison of gene, enzymatic, and biological activities with those of Clostridium perfringens alpha-toxin. (2013)
  • Author(s): Ni Nengah Dwi Fatmawati, Y. Sakaguchi, T. Suzuki, M. Oda, K. Shimizu, Y. Yamamoto, J. Sakurai, O. Matsushita and K. Oguma
  • Journal Title: Acta Medica Okayama Volume: 67 Pages: 9-18