Study for structure and function of botulinum hemagglutinin
Project/Area Number |
25860320
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Bacteriology (including mycology)
|
Research Institution | Tokyo University of Agriculture (2014-2015) Okayama University (2013) |
Principal Investigator |
Suzuki Tomonori 東京農業大学, 応用生物科学部, 助教 (90453836)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Keywords | Clostridium botulinum / 毒素複合体 / ボツリヌス毒素 / 血球凝集素 / タンパク質間相互作用 / タンパク質複合体 / 立体構造 / 立体構造予測 |
Outline of Final Research Achievements |
The large toxin complex (L-TC) produced by Clostridium botulinum is formed from neurotoxin and four non-toxic proteins. In particular, HA components appear to play a role in the transport of the TC across the intestinal epithelium. In this study, the binding regions between HA subcomponents were examined.
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Report
(4 results)
Research Products
(6 results)
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[Journal Article] Identification of the interaction region between hemagglutinin components of the botulinum toxin complex2014
Author(s)
Suzuki T, Miyashita S, Hayashi S, Miyata K, Inui K, Kondo Y, Miyazaki S, Ohyama T, Niwa K, Watanabe T, Sagane Y
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Journal Title
International Journal of Biological Macromolecules
Volume: 65
Pages: 284-288
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Sugar-induced conformational change found in the HA-33/HA-17 trimer of the botulinum toxin complex2013
Author(s)
Yoshimasa Sagane, Shintaro Hayash, Takashi Matsumoto, Shin-ichro Miyashita, Ken Inui, Keita Miyata, Shunsuke Yajima, Tomonori Suzuki, Kimiko Hasegawa, Akihito Yamano, Atushi Nishikawa, Tohru Ohyama, Toshihiro Watanabe, Koichi Niwa
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Journal Title
Biochem. Biophys. Res. Commun
Volume: 438
Issue: 3
Pages: 483-487
DOI
Related Report
Peer Reviewed
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[Journal Article] Random phage display based screening of peptides that bind to botulinum neurotoxin binding protein, nontoxic nonhemagglutinin.2013
Author(s)
Sagane Y, Miyata K, Kurihara S, Yoneyama T, Inui K, Miyashita S, Hayashi S, Suzuki T, Niwa K,
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Journal Title
Current Microbiology
Volume: (Online)
Issue: 2
Pages: 188-192
DOI
Related Report
Peer Reviewed
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