Pathogenic analysis of S. aureus isolated from Staphylococcal Scaled skin syndrome

• Project/Area Number: 25860321
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including mycology)
• Research Institution: Hiroshima University
• Principal Investigator: HISATSUNE Junzo 広島大学, 医歯薬保健学研究院, 助教 (40513180)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 細菌 / ゲノム / タンパク質 / 微生物 / 蛋白質 / 進化

Outline of Final Research Achievements

On the investigation A), we found that A domain of Skip deletion mutant strain was not digested by human serum and human plasmin. And then, we found that cutting site of Skip have about N terminus 20 amino acid in A domain of Skip.On the investigation B), highly expressing Skip strain lacked 6-nucleotides in internecine region of arT and sarU. We found that global regulator MgrA binds to the region containing 6-nucleotides and lack of the short stretch prevents the binding of the MgrA, hence expression of SarT/SarU induces expression of Skip. In this study, we uncovered one of the reason why the strains causing SSSS shows high expression of Skip.

Research Products

• [Journal Article] Tripoli metallo-β-lactamase-1 (TMB-1)-producing Acinetobacter spp. with decreased resistance to imipenem in Japan. (2014)
  • Author(s): Kayama S, Shigemoto N, Shimizu W, Kuwahara R, Ikeda M, Ikebe K, Maeda K, Hisatsune J, Ohge H, Sugai M.
  • Journal Title: Antimicrob Agents Chemother. Volume: 58 Issue: 4 Pages: 2477-2478
  • DOI: 10.1128/aac.01790-13
  • Peer Reviewed / Open Access
• [Journal Article] Comprehensive analysis of systemically disseminated ST8/non-USA300 type community-acquired methicillin-resistant Staphylococcus aureus infection. (2014)
  • Author(s): Hagiya H, Hisatsune J, Kojima T, Shiota S, Naito H, Hagioka S, Morimoto N, Otsuka F, Sugai M.
  • Journal Title: Intern Med. Volume: 53 Pages: 907-912
  • Peer Reviewed / Open Access
• [Journal Article] Phospholipase C-related catalytically inactive protein participates in the autophagic elimination of Staphylococcus aureus infecting mouse embryonic fibroblasts. (2014)
  • Author(s): Harada-Hada K, Harada K, Kato F, Hisatsune J, Tanida I, Ogawa M, Asano S, Sugai M, Hirata M, Kanematsu T.
  • Journal Title: PLoS One. Volume: 9 Issue: 5 Pages: e98285-e98285
  • DOI: 10.1371/journal.pone.0098285
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Application of moisturizer to neonates prevents development of atopic dermatitis. (2014)
  • Author(s): Horimukai K, Morita K, Narita M, Kondo M, Kitazawa H, Nozaki M, Shigematsu Y, Yoshida K, Niizeki H, Motomura K, Sago H, Takimoto T, Inoue E, Kamemura N, Kido H, Hisatsune J, Sugai M, Murota H, Katayama I, Sasaki T, Amagai M, Morita H, Matsuda A, Matsumoto K, Saito H, Ohya Y.
  • Journal Title: The Journal of allergy and clinical immunology. Volume: 134 Issue: 4 Pages: 824-830
  • DOI: 10.1016/j.jaci.2014.07.060
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Emergence of Staphylococcus aureus carrying multiple drug resistance genes on a plasmid encoding exfoliative toxin B. (2013)
  • Author(s): Hisatsune J, Hirakawa H, Yamagichi T, Fudaba Y, Oshima K, Hattori M, Kato F, Kayama S, Sugai M
  • Journal Title: Antimicrobial Agents and Chemotherapy Volume: 57 Issue: 12 Pages: 6131-6140
  • DOI: 10.1128/aac.01062-13
  • Peer Reviewed
• [Journal Article] 解説 黄色ブドウ球菌 (2013)
  • Author(s): 久恒順三, 達川伸行, 佐藤祐介, 加藤文紀, 鹿山鎭男, 菅井基行
  • Journal Title: 感染症内科 Volume: 1 Pages: 275-285
• [Presentation] おでき由来黄色ブドウ球菌が産生するヒアルロン酸分解酵素の解析 (2015)
  • Author(s): 窪田星子, 達川伸行, 久恒順三, 金子淳, 菅井基行
  • Organizer: 第88回日本細菌学会総会
  • Place of Presentation: 長良川国際会議場
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] 黄色ブドウ球菌の表層タンパク質 Skip の発現制御機構の解析 (2014)
  • Author(s): 達川伸行, 久恒順三, 林幾江, 菅井基行
  • Organizer: 第67回日本細菌学会中国・四国支部総会
  • Place of Presentation: 徳島文理大学
  • Year and Date: 2014-10-04 – 2014-10-05
• [Presentation] Skip, a cell wall protein of S. aureus for biphasic skin adhesion strategies (2014)
  • Author(s): Junzo Hisatsune, Teruaki Murakami, Nobuyuki Tatsukawa, Ikue Hayashi, Motoyuki Sugai
  • Organizer: 16th International Symposium on Staphylococci and Staphylococcal Infections
  • Place of Presentation: Chicago Palmer House Hilton (USA)
  • Year and Date: 2014-08-26 – 2014-08-29
• [Presentation] Regulatory mechanism of cell wall protein Skip in S. aureus (2014)
  • Author(s): Nobuyuki Tatsukawa, Junzo Hisatsune, Ikue Hayashi, Motoyuki Sugai
  • Organizer: 16th International Symposium on Staphylococci and Staphylococcal Infections
  • Place of Presentation: Chicago Palmer House Hilton (USA)
  • Year and Date: 2014-08-26 – 2014-08-29
• [Presentation] 犬膿皮症由来 S.pseudintermedius 表皮剥脱毒素 ExpB 産生株のゲノム解析 (2014)
  • Author(s): 久恒 順三, 平川英樹, 伊從慶太, 西藤公司, 大島健志朗, 服部 正平, 菅井基行
  • Organizer: 第59回日本ブドウ球菌研究会
  • Place of Presentation: 東京農工大学 農学部第1講義棟 16 号室
  • Year and Date: 2014-08-04
• [Presentation] Impetigo/SSSS原性S. aureusが産生する表層蛋白質Skipの皮膚定着機序の解析 (2014)
  • Author(s): 久恒順三, 村上輝明, 小島太郎, 達川伸行, 林幾江, 山田作夫, 菅井基行
  • Organizer: 第８７回日本細菌学会総会
  • Place of Presentation: 東京
  • Invited
• [Remarks] 広島大学大学院医歯薬保健学研究科医歯薬学専攻細菌学教室
  • URL: http://home.hiroshima-u.ac.jp/saikin/index.html
• [Remarks] 広島大学大学院医歯薬保健学研究科医歯薬学専攻細菌学教室
  • URL: http://home.hiroshima-u.ac.jp/saikin/index.html