To clarify why the stability of CDT-I prophage is different between Escherichia coli belonging to different O serogroups

• Project/Area Number: 25860323
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including mycology)
• Research Institution: Osaka Prefecture University
• Principal Investigator: Atsushi Hinenoya 大阪府立大学, 生命環境科学研究科(系), 助教 (20548490)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: CDT / 大腸菌 / ファージ / spontaneous induction / 細胞膨化致死毒素 / CDT-Iファージ / Spontaneous induction

Outline of Final Research Achievements

Cytolethal distending toxin (CDT-I) produced by Escherichia coli is encoded on lambdoid phage, and its phage induction is associated with the toxin production. We found that the induction level of CDT-I phage is different among the strains, and correlated with O serogroup of host E. coli including O127 and O142. The present study tried to identify the host factor which regulate CDT-I phage induction. Finally although we could not identify it during this research period, new findings could be obtained: (1) CDT-I phage induction is occurred in a RecA dependent pathway, and (2) intra-prophage interaction could be involved in the different CDT-I phage induction between CDT-I-producing E. coli O serogroups O127 and O142.

Research Products

• [Journal Article] Chlorine Dioxide is a Better Disinfectant than Sodium Hypochlorite against Multi-Drug Resistant <i>Staphylococcus aureus</i>, <i>Pseudomonas aeruginosa</i>, and <i>Acinetobacter baumannii</i> (2015)
  • Author(s): A. Hinenoya, S.P. Awasthi, N. Yasuda, A. Shima, H. Morino, T. Koizumi, T. Fukuda, T. Miura, T. Shibata, S. Yamasaki
  • Journal Title: Japanese Journal of Infectious Diseases Volume: 68 Issue: 4 Pages: 276-279
  • DOI: 10.7883/yoken.JJID.2014.294
  • NAID: 130005088795
  • ISSN: 1344-6304, 1884-2836
  • Peer Reviewed
• [Journal Article] Anethole inhibits growth of recently emerged multidrug resistant toxigenic <i>Vibrio cholerae</i> O1 El Tor variant strains <i>in vitro</i> (2015)
  • Author(s): M.S.H. Zahid, S.P. Awasthi, A. Hinenoya, and S. Yamasaki
  • Journal Title: Journal of Veterinary Medical Science Volume: 77 Issue: 5 Pages: 535-540
  • DOI: 10.1292/jvms.14-0664
  • NAID: 130005071874
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] Campylobacter hyointestinalis isolated from pigs produces multiple variants of biologically active cytolethal distending toxin (2015)
  • Author(s): K. Kamei, N. Hatanaka, M. Asakura, S. Somroop, W. Samosornsuk, A. Hinenoya, N. Misawa, S. Nakagawa, S. Yamasaki
  • Journal Title: Infect. Immun. Volume: 83 Issue: 11 Pages: 4304-4313
  • DOI: 10.1128/iai.00997-15
  • Peer Reviewed
• [Journal Article] Suppression of virulence of toxigenic Vibrio cholerae by anethole through the cyclic AMP (cAMP)-cAMP receptor protein signaling system (2015)
  • Author(s): M.S.H. Zahid, S.P. Awasthi, M. Asakura, S. Chatterjee, A. Hinenoya, S. M. Faruque and S. Yamasaki
  • Journal Title: PLoS ONE Volume: 10 Issue: 9 Pages: 137529-137529
  • DOI: 10.1371/journal.pone.0137529
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Prevalence and pathogenic potential of Escherichia coli isolates from raw milk and raw milk cheese in Egypt (2015)
  • Author(s): R.A. Ombarak, A. Hinenoya, S.P. Awasthi, A. Iguchi, A. Shima, A.R. Elbagory, S. Yamasaki
  • Journal Title: Int. J. Food. Microbiol. Volume: 221 Pages: 69-76
  • DOI: 10.1016/j.ijfoodmicro.2016.01.009
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Chlorine dioxide is a superior disinfectant against multi-drug resistant Staphylococcus aureus, Pseudomonas aeruginosa and Acinetobacter baumannii (2015)
  • Author(s): A. Hinenoya, S.P. Awasthi, N. Yasuda, A. Shima, H. Morino, T. Koizumi, T. Fukuda, T. Miura, T. Shibata, S. Yamasaki
  • Journal Title: Jpn. J. Infect. Dis Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Anethole inhibits growth of recently emerged multidrug resistant toxigenic Vibrio cholerae O1 El Tor variant strains in vitro (2015)
  • Author(s): M.S. Zahid, S.P. Awasthi, A. Hinenoya, S. Yamasaki
  • Journal Title: J. Vet. Med. Sci Volume: 印刷中
  • NAID: 130005071874
  • Peer Reviewed
• [Journal Article] Molecular characterization of cytolethal distending toxin gene-positive Escherichia coli from healthy cattle and swine in Nara, Japan (2014)
  • Author(s): A. Hinenoya, K. Shima, M. Asakura, K. Nishimura, T. Tsukamoto, T. Ooka, T. Hayashi, T. Ramamurthy, S.M. Faruque and S. Yamasaki
  • Journal Title: BMC Microbiol. Volume: 14 Issue: 1 Pages: 97-97
  • DOI: 10.1186/1471-2180-14-97
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Development of a PCR-restriction fragment length polymorphism assay for detection and subtyping of cholix toxin variant genes of Vibrio cholerae (2014)
  • Author(s): Awasthi SP, Asakura M, Neogi SB, Hinenoya A, Ramamurthy T, Yamasaki S
  • Journal Title: J Med Microbiol Volume: 63 Issue: 5 Pages: 659-666
  • DOI: 10.1099/jmm.0.070797-0
  • Peer Reviewed
• [Journal Article] A PCR-RFLP assay for the detection and differentiation of Campylobacter jejuni, C. coli, C. fetus, C. hyointestinalis, C. lari, C. helveticus and C. upsaliensis (2014)
  • Author(s): K. Kamei, M. Asakura, S. Somroop, A. Hinenoya, A. Nagita, N. Misawa, M. Matsuda, S. Nakagawa, S. Yamasaki
  • Journal Title: J. Med. Microbiol Volume: not available Issue: 5 Pages: 659-666
  • DOI: 10.1099/jmm.0.071498-0
  • Peer Reviewed
• [Journal Article] Prevalence of Vibrio cholerae O1 El Tor variant in a cholera-endemic zone of Kenya (2014)
  • Author(s): Saidi SM1, Chowdhury N, Awasthi SP, Asakura M, Hinenoya A, Iijima Y, Yamasaki S
  • Journal Title: J Med Microbiol Volume: 63 Issue: 3 Pages: 415-420
  • DOI: 10.1099/jmm.0.068999-0
  • Peer Reviewed
• [Presentation] ベトナム、メコンデルタ地区における家畜からのESBL産生大腸菌の分離と性状解析 (2015)
  • Author(s): 日根野谷淳、Tran Thi Thu Suong、Nguyen Trong Ngu、Nguyen Cong Ha、Nguyen Doan Duy、Hoai Hoang Phuong、住村欣範、山本容正、山﨑伸二
  • Organizer: 第36回日本食品微生物学会学術総会
  • Place of Presentation: 川崎市教育文化会館（神奈川、川崎）
  • Year and Date: 2015-11-12
• [Presentation] Analysis of CDT-I phage production in Escherichia coli (2015)
  • Author(s): A. Hinenoya, S.P. Awasthi, M. Asakura, S. Yamasaki
  • Organizer: FEMS 6th Congress of European Microbiologists
  • Place of Presentation: Maastricht (The Netherlands)
  • Year and Date: 2015-06-07
  • Int'l Joint Research
• [Presentation] Phenotypic analysis of CDT-I phages in Escherichia coli (2015)
  • Author(s): A. Hinenoya, M. Asakura, S. Yamasaki
  • Organizer: 第88回日本細菌学会総会
  • Place of Presentation: 長良川国際会議場（岐阜）
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] 鶏肉のカンピロバクター汚染と検査法の問題点 (2015)
  • Author(s): 日根野谷淳
  • Organizer: 第43回日本食品微生物学会学術セミナー
  • Place of Presentation: 京都商工会議所（京都）
  • Year and Date: 2015-03-13
  • Invited
• [Presentation] Campylobacter属8菌種の同定を行うためのPCR-RFLP法の開発 (2014)
  • Author(s): 畑中律敏、平藤優子、亀井数正、S. Somroop、日根野谷淳、朝倉昌博、名木田章、山崎伸二
  • Organizer: 第7回日本カンピロバクター研究会総会
  • Place of Presentation: 国立医薬品食品衛生研究所（東京）
  • Year and Date: 2014-12-11 – 2014-12-12
• [Presentation] アネトールはin vitro及びin vivoで多剤耐性コレラ菌O1エルトールバリアントの病原性発現を抑制する (2014)
  • Author(s): M.S. Zahid、S.P. Awasthi、朝倉昌博、日根野谷淳、S.M. Faruque、山崎伸二
  • Organizer: 第48回腸炎ビブリオシンポジウム
  • Place of Presentation: 金森商船（株） 函館ヒストリープラザ（北海道）
  • Year and Date: 2014-11-13 – 2014-11-14
• [Presentation] Persistent gastrointestinal colonization of ESBL-producing Escherichia coli in cefoperazone treated mice (2014)
  • Author(s): H.H. Phuong, N. Yasuda, I. Hirai, Y. Yamamoto, S.P. Awasthi, A. Hinenoya, S. Yamasaki
  • Organizer: The 1st International Allied Health Sciences Conference
  • Place of Presentation: Bangkok (Thailand)
  • Year and Date: 2014-11-04 – 2014-11-06
• [Presentation] アルゼンチンにおける肉用牛からの志賀毒素産生性大腸菌の分離と性状解析 (2014)
  • Author(s): 奥野健太郎、Germán A. Kopprio、Rubén J. Lara、日根野谷淳、山崎伸二
  • Organizer: 第18回腸管出血性大腸菌感染症研究会
  • Place of Presentation: 同志社大学（京都）
  • Year and Date: 2014-07-15 – 2014-07-16
• [Presentation] Characterization of CDT-I phages in Escherichia coli (2014)
  • Author(s): A. Hinenoya, M. Asakura, S. Yamasaki
  • Organizer: The 114th general meeting of American Society for Microbiology
  • Place of Presentation: Boston (USA)
  • Year and Date: 2014-05-17 – 2014-05-20
• [Presentation] Genetic analysis of horizontal transfer of CDT-I phages among Escherichia coli (2014)
  • Author(s): Hinenoya A, Asakura M, Yamasaki S
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: タワーホール船堀
• [Presentation] アルゼンチンで分離された志賀毒素産生性大腸菌の分子疫学的解析 (2013)
  • Author(s): 奥野健太郎、German A. Kopprio、Ruben J. Lara、日根野谷淳、山崎伸二
  • Organizer: 第66回日本細菌学会関西支部総会
  • Place of Presentation: 大阪大学微生物病研究所
• [Presentation] 抗菌薬投与が薬剤耐性菌のマウスの排菌期間に及ぼす影響 (2013)
  • Author(s): Hoang Hoai Phuong、安田憲朋、平井到、山本容正、Sharda Prasad Awasthi、日根野谷淳、山崎伸二
  • Organizer: 第66回日本細菌学会関西支部総会
  • Place of Presentation: 大阪大学微生物病研究所
• [Presentation] Escherichia coli harboring cytolethal distending toxin-II gene is actually E. albertii (2013)
  • Author(s): Yasuda N, Hinenoya A, Nagita A, Hibino T, Mukaizawa N, Niwa Y, Tsukamoto T, Asakura M, Yamasaki S
  • Organizer: The 113th general meeting of American Society for Microbiology
  • Place of Presentation: Denver, Colorado, USA
• [Presentation] 3型および5型細胞膨化致死毒素遺伝子の特異的PCRの構築 (2013)
  • Author(s): 日根野谷淳、山崎伸二
  • Organizer: 第17回腸管出血性大腸菌感染症研究会
  • Place of Presentation: つくば農林ホール