Cell-layer transport mechanism of botulinum neurotoxin-binding protein, nontoxic nonhemagglutinin
| Project/Area Number |
25860325
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | Tokyo University of Agriculture |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | ボツリヌス食中毒 / 体内輸送 / 経口毒素 / タンパク質毒素 / 消化管輸送 |
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| Outline of Final Research Achievements |
Botulinum toxin (BoNT) complex (TC) traverses the gastrointestinal tract and circulation, eventually becoming localized in neuromuscular junctions. During this process, BoNT must pass through cells, thus from the intestinal lumen to the cells of the intestinal tract and blood vessels. The botulinum TC is formed by association of the BoNT with at least one nontoxic protein, nontoxic nonhemagglutinin (NTNHA). In the present work, we examined the binding and transcytosis of serotype D NTNHA protein in epithelial and endothelial cells to clarify the role played by the protein in toxin delivery. Our studies showed that NTNHA bound to and transcytosed across IEC-6 and BAEC cells. While NTNHA also bound to MDCK or Caco-2 cells, but did not traverse across MDCK or Caco-2 cells. Such specificity of NTNHA protein transcytosis may explain why only some animals are sensitive to botulinum toxin. The sensitivity depends on the toxin serotype in play, and the route of toxin delivery.
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Report
(3 results)
Research Products
(14 results)
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[Journal Article] 87. Purification, crystallization and preliminary X-ray analysis of a HA17-HA70 (HA2-HA3) complex from Clostridium botulinum type C progenitor toxin.2014
Author(s)
Chikako Iwasa, Takashi Tonozuka, Masaya Shinoda, Yoshimasa Sagane, Koichi Niwa, Toshihiro Watanabe, Hiromi Yoshida, Shigehiro Kamitori, Toshifumi Takao, Keiji Oguma, Atsushi Nishikawa
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Journal Title
Acta Crystallographica
Volume: F70
Issue: 1
Pages: 64-67
DOI
Related Report
Peer Reviewed
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[Journal Article] Identification of the interaction region between hemagglutinin components of the botulinum toxin complex2014
Author(s)
Suzuki T, Miyashita S, Hayashi S, Miyata K, Inui K, Kondo Y, Miyazaki S, Ohyama T, Niwa K, Watanabe T, Sagane Y
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Journal Title
International Journal of Biological Macromolecules
Volume: 65
Pages: 284-288
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Sugar-induced conformational change found in the HA-33/HA-17 trimer of the botulinum toxin complex2013
Author(s)
Yoshimasa Sagane, Shintaro Hayash, Takashi Matsumoto, Shin-ichro Miyashita, Ken Inui, Keita Miyata, Shunsuke Yajima, Tomonori Suzuki, Kimiko Hasegawa, Akihito Yamano, Atushi Nishikawa, Tohru Ohyama, Toshihiro Watanabe, Koichi Niwa
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Journal Title
Biochem. Biophys. Res. Commun
Volume: 438
Issue: 3
Pages: 483-487
DOI
Related Report
Peer Reviewed
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