Development of vaccine for Clostridium difficile infection using membrane fraction
| Project/Area Number |
25860327
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
Senoh Mitsutoshi 国立感染症研究所, その他部局等, 主任研究官 (20646624)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | Clostridium difficile / ワクチン / 定着因子 / Membrane fraction / Vaccine |
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| Outline of Final Research Achievements |
Diarrhea and pseudomembrane colitis caused by Clostridium difficile infection (CDI) is a global health concern because of the high recurrence rate after standard antibiotic therapy. Vaccination presents a powerful countermeasure against disease recurrence. In this study, mice vaccinated with the nontoxigenic C. difficile membrane fraction (ntCDMF) generated a marked immune response to the antigen, as demonstrated by the serum IgG and intestinal fluid IgA levels. Significantly, pretreatment with harvested IgG- and IgA-containing fluids was sufficient to prevent in vitro adhesion of C. difficile to Caco-2 cells. Furthermore, it was examined whether ntCDMF has an effect on in vivo situation. The number of C. difficile in feces of mice that were immunized by ntCDMF decreased than that of control mice. The survived number of hamsters that were immunized by ntCDMF was more than that of control hamsters. These results highlight the potential of ntCDMF as a vaccine candidate for CDI.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] Predominance of PCR-ribotypes, 018 (smz) and 369 (trf) of Clostridium difficile in Japan: a potential relationship with other global circulating strains?2015
Author(s)
Senoh M, Kato H, Fukuda T, Niikawa A, Hori Y, Hagiya H, Ito Y, Miki H, Abe Y, Furuta K, Takeuchi H, Tajima H, Tominaga H, Satomura H, Kato H, Morita S, Tanada A, Hara T, Kawada M, Sato Y, Takahashi M, Higuchi A, Nakajima T, Wakamatsu Y, Toyokawa M, Ueda A, Roberts P, Miyajima F, Shibayama K.
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Journal Title
J Med Microbiol.
Volume: 64
Issue: 10
Pages: 1226-1236
DOI
Related Report
Peer Reviewed
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[Journal Article] Postoperative Clostridium difficile infection with PCR ribotype 078 strain identified at necropsy in five Thoroughbred racehorses.2013
Author(s)
Niwa H, Kato H, Hobo S, Kinoshita Y, Ueno T, Katayama Y, Hariu K, Oku K, Senoh M, Kuroda T, Nakai K.
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Journal Title
Vet Rec
Volume: 173
Issue: 24
Pages: 607-607
DOI
Related Report
Peer Reviewed
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