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Development of vaccine for Clostridium difficile infection using membrane fraction

Research Project

Project/Area Number 25860327
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Bacteriology (including mycology)
Research InstitutionNational Institute of Infectious Diseases

Principal Investigator

Senoh Mitsutoshi  国立感染症研究所, その他部局等, 主任研究官 (20646624)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsClostridium difficile / ワクチン / 定着因子 / Membrane fraction / Vaccine
Outline of Final Research Achievements

Diarrhea and pseudomembrane colitis caused by Clostridium difficile infection (CDI) is a global health concern because of the high recurrence rate after standard antibiotic therapy. Vaccination presents a powerful countermeasure against disease recurrence. In this study, mice vaccinated with the nontoxigenic C. difficile membrane fraction (ntCDMF) generated a marked immune response to the antigen, as demonstrated by the serum IgG and intestinal fluid IgA levels. Significantly, pretreatment with harvested IgG- and IgA-containing fluids was sufficient to prevent in vitro adhesion of C. difficile to Caco-2 cells. Furthermore, it was examined whether ntCDMF has an effect on in vivo situation. The number of C. difficile in feces of mice that were immunized by ntCDMF decreased than that of control mice. The survived number of hamsters that were immunized by ntCDMF was more than that of control hamsters. These results highlight the potential of ntCDMF as a vaccine candidate for CDI.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (13 results)

All 2016 2015 2014 2013

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Acknowledgement Compliant: 1 results) Presentation (7 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Predominance of PCR-ribotypes, 018 (smz) and 369 (trf) of Clostridium difficile in Japan: a potential relationship with other global circulating strains?2015

    • Author(s)
      Senoh M, Kato H, Fukuda T, Niikawa A, Hori Y, Hagiya H, Ito Y, Miki H, Abe Y, Furuta K, Takeuchi H, Tajima H, Tominaga H, Satomura H, Kato H, Morita S, Tanada A, Hara T, Kawada M, Sato Y, Takahashi M, Higuchi A, Nakajima T, Wakamatsu Y, Toyokawa M, Ueda A, Roberts P, Miyajima F, Shibayama K.
    • Journal Title

      J Med Microbiol.

      Volume: 64 Issue: 10 Pages: 1226-1236

    • DOI

      10.1099/jmm.0.000149

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of adhesion of Clostridium difficile to human intestinal cells after treatment with serum and intestinal fluid isolated from mice immunized with nontoxigenic C. difficile membrane fraction.2015

    • Author(s)
      Senoh M, Iwaki M, Yamamoto A, Kato H, Fukuda T, Shibayama K.
    • Journal Title

      Microb Pathog.

      Volume: 81 Pages: 1-5

    • DOI

      10.1016/j.micpath.2015.03.001

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Fulminant pseudomembranous colitis caused by Clostridium difficile PCR ribotype 027 in a healthy young woman in Japan.2014

    • Author(s)
      Nishimura S, Kou T, Kato H, Watanabe M, Uno S, Senoh M, Fukuda T, Hata A, Yazumi S.
    • Journal Title

      J Infect Chemother.

      Volume: 20 Issue: 11 Pages: 729-731

    • DOI

      10.1016/j.jiac.2014.07.004

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Reverse transcription polymerase chain reaction-based method for selectively detecting vegetative cells of toxigenic Clostridium difficile.2014

    • Author(s)
      Senoh M, Kato H, Murase T, Hagiya H, Tagashira Y, Fukuda T, Iwaki M, Yamamoto A, Shibayama K.
    • Journal Title

      Microbiol Immunol.

      Volume: 58 Issue: 11 Pages: 615-620

    • DOI

      10.1111/1348-0421.12189

    • NAID

      40020271468

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Postoperative Clostridium difficile infection with PCR ribotype 078 strain identified at necropsy in five Thoroughbred racehorses.2013

    • Author(s)
      Niwa H, Kato H, Hobo S, Kinoshita Y, Ueno T, Katayama Y, Hariu K, Oku K, Senoh M, Kuroda T, Nakai K.
    • Journal Title

      Vet Rec

      Volume: 173 Issue: 24 Pages: 607-607

    • DOI

      10.1136/vr.101960

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Two cases of fulminant colitis due to binary toxin-positive Clostridium difficile that are not PCR ribotype 027 or type 078.2013

    • Author(s)
      Tagashira Y, Kato H, Senoh M, Nakamura A.
    • Journal Title

      J Med Microbiol

      Volume: 62 Issue: 9 Pages: 1486-1489

    • DOI

      10.1099/jmm.0.057968-0

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 膜画分を用いたClostridium difficile感染症(CDI)ワクチンのin vivo効果2016

    • Author(s)
      妹尾充敏, 岩城正昭, 山本明彦, 加藤はる, 福田靖, 柴山恵吾
    • Organizer
      第89回日本細菌学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2016-03-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] Nontoxigenic Clostridium difficile membrane fraction as a vaccine candidate.2015

    • Author(s)
      Senoh M, Iwaki M, Yamamoto A, Kato H, Fukuda T, Shibayama K.
    • Organizer
      The 6th Congress of European Microbiologists
    • Place of Presentation
      Maastricht, Netherlands
    • Year and Date
      2015-06-07
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Prevalence of two PCR ribotypes, smz (180) and trf (369) of Clostridium difficile in JAPAN.2015

    • Author(s)
      Kato H, Senoh M, Fukuda T, Roberts P, Miyajima F, Shibayama K.
    • Organizer
      5th International Clostridium difficile Symposium
    • Place of Presentation
      Bled, Slovenia
    • Year and Date
      2015-05-19
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 膜画分を用いたClostridium difficile感染症(CDI)ワクチンの開発2015

    • Author(s)
      妹尾充敏, 岩城正昭, 山本明彦, 加藤はる, 福田靖, 柴山恵吾
    • Organizer
      第88回日本細菌学会総会
    • Place of Presentation
      岐阜
    • Year and Date
      2015-03-26 – 2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] 毒素産生性Clostridium difficileの新規遺伝学的検査法の開発2014

    • Author(s)
      妹尾充敏、加藤はる、福田靖、柴山恵吾
    • Organizer
      第61回トキシンシンポジウム
    • Place of Presentation
      鳴門
    • Year and Date
      2014-09-03 – 2014-09-05
    • Related Report
      2014 Research-status Report
  • [Presentation] 毒素産生性Clostridium difficile栄養型菌の新規遺伝学的検査法の開発2014

    • Author(s)
      妹尾充敏、加藤はる、福田靖、柴山恵吾
    • Organizer
      第88回日本感染症学会学術講演会 第62回日本化学療法学会総会 合同学会
    • Place of Presentation
      博多
    • Year and Date
      2014-06-18 – 2014-06-20
    • Related Report
      2014 Research-status Report
  • [Presentation] 日本の医療施設におけるClostridium difficile感染症の疫学調査2014

    • Author(s)
      妹尾充敏、加藤はる、福田靖、柴山恵吾
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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