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Bordetella pertussis fimbriae are regulated by BvgAS system and Pfim structure

Research Project

Project/Area Number 25860328
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Bacteriology (including mycology)
Research InstitutionNational Institute of Infectious Diseases

Principal Investigator

OTSUKA Nao  国立感染症研究所, その他部局等, 研究員 (90596610)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords百日咳菌 / fimbriae / 発現機構 / 発現 / 遺伝子発現
Outline of Final Research Achievements

Bordetella pertussis, the etiologic agent of whooping cough, produces two serologically distinct fimbriae, Fim2 and Fim3 either one or both. Expression of fimbrial genes fim2 and fim3 is regulated by the two component BvgAS system, and by the length of poly(C) tract in the promoter of each gene. In this study, we observed that the Pfim poly(C) distribution does not be affected by growing medium and antibacterial agents with sub-MIC. In the Bvg+ phase, B. pertussis virulence-activated genes (vags) are up-regulated, and virulence-repressed genes (vrgs) are down-regulated. We confirmed that fim2 gene is a vag as previous studies reported, but fim3 behaved as either a vag or vrg, depending on the Pfim3 poly(C) length and Bvg state.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (4 results)

All 2015 2013

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (1 results) Book (1 results)

  • [Journal Article] Molecular epidemiology of Bordetella pertussis in the Philippines in 2012-20142015

    • Author(s)
      Galit SR, Otsuka N, Furuse Y, et al.
    • Journal Title

      Int J Infect Dis

      Volume: 35 Pages: 24-26

    • DOI

      10.1016/j.ijid.2015.04.001

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genetic analysis of Bordetella pertussis isolates from the 2008-2010 pertussis epidemic in Japan2013

    • Author(s)
      Miyaji Y, Otsuka N, Toyoizumi-Ajisaka H, Shibayama K, Kamachi K
    • Journal Title

      PLoS One

      Volume: (8)10 Issue: 10 Pages: e77165-e77165

    • DOI

      10.1371/journal.pone.0077165

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Bordetella pertussis fimbriae are regulated by BvgAS system and Pfim structure2015

    • Author(s)
      Nao Otsuka, Valerie Bouchez, Keigo Shibayama, Kazunari Kamachi, Nicole Guiso
    • Organizer
      第88回日本細菌学会総会
    • Place of Presentation
      岐阜県岐阜市
    • Year and Date
      2015-03-26 – 2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Book] 化学療法の領域 2013年 増刊号(Vol.29 S-1):細菌感染症 8.百日咳2013

    • Author(s)
      大塚菜緒、蒲地一成
    • Total Pages
      9
    • Publisher
      医薬ジャーナル
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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