Analysis of hemorrhagic fever virus assembly and budding by visible technology

Research Project

Project/Area Number	25860342
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Virology
Research Institution	Nagasaki University
Principal Investigator	URATA Shuzo 長崎大学, 熱帯医学研究所, 助教 (20614449)
Project Period (FY)	2013-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000) Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	ウイルス
Outline of Final Research Achievements	1. Lassa virus Z has a mojor role on virus assembly and budding. We have shown that Z localized at late endosome(CD63 as a marker), and viral glycoprotein (GPC) facilitated this localization significantly. 2. Cellular factor, ALIX/AIP1, plays an important role on Z-mdeiated virus budding. 3. Z was tagged with Nano luc (Nluc) gene to establish high-through put screening system, and A549 stably expressing Z-Nluc cell line was cloned.