| Project/Area Number |
25860344
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Virology
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| Research Institution | Jichi Medical University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | E型肝炎ウイルス / 5'非翻訳領域 / ウイルス複製 / リバースジェネティクス法 / リアルタイムRT-PCR法 / cell-free蛋白質翻訳系 |
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| Outline of Final Research Achievements |
To investigate the functional role of 5' untranslated region (5’UTR) of the genome RNA in the replication of hepatitis E virus (HEV), various 5’UTR mutants were constructed and their full-length RNA were transfected into PLC/PRF/5 cells, Influence of 5’UTR mutations on replication efficiency was evaluated by various methods such as real-time RT-PCR. All HEV mutants lacking secondary structure in the 5’UTR could not grow in cells, indicating that the secondary structure of the 5’UTR plays an essential role for viral replication. In addition, some mutants with mutations in stem and/or bulge structures replicated less efficiently, suggesting that the primary structure of the 5’UTR is also important for active replication of HEV.
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