Role of memory T helper cells and their maintenance in vivo

• Project/Area Number: 25860352
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Immunology
• Research Institution: Chiba University
• Principal Investigator: SHINODA Kenta 千葉大学, 医学(系)研究科(研究院), 助教 (10612195)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: アレルギー･喘息 / 免疫学 / アレルギー・ぜんそく

Outline of Final Research Achievements

To investigate the roles of bone marrow (BM) memory T helper (Th) cells, we evaluated humoral immune response of CD69-deficient mice which lacks them. We have clarified that BM memory Th cells are essential for efficient establishment for humoral immunity. On the other hands, it is known that there are memory Th cells that maintained at peripheral non-lymphoid tissues which are the sites of pathogen invasion. We focused on the maintenance of memory Th cells in lung tissue and found that memory Th cells are maintained within inducible bronchus-associated lymphoid tissue (iBALT). These studies will contribute the understanding of the role of memory Th cells and their maintenance in the body.

Research Products

• [Journal Article] Trithorax complex component Menin controls differentiation and maintenance of T helper 17 cells. (2014)
  • Author(s): Watanabe, Y., Onodera, A., Kanai, U., Ichikawa, T., Obata-Ninomiya, K., Wada, T., Kiuchi, M., Iwamura, C., Tumes, DJ., Shinoda, K., Yagi, R., Motohashi, S., Hirahara, K. and Nakayama, T.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 111(35) Issue: 35 Pages: 12829-34
  • DOI: 10.1073/pnas.1321245111
  • Peer Reviewed / Open Access
• [Journal Article] The Interleukin-33-p38 kinase axis confers memory T helper 2 cell pathogenicity in the airway. (2014)
  • Author(s): Endo, Y., Hirahara, K., Iinuma, T., Shinoda, K., Tumes, D. J., Asou, K. H., Matsugae, N., Obata-Ninomiya, K., Yamamoto, H., Motohashi, S., Oboki, K., Nakae, S., Saito, H., Okamoto, Y., and Nakayama, T.
  • Journal Title: Immunity Volume: 42 Pages: 294-308
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The polycomb protein Ezh2 regulates differentiation and plasticity of CD4+ T helper type-1 and type-2 cells. (2013)
  • Author(s): Tumes, D. J., Onodera, A., Suzuki, A., Shinoda, K., Endo, Y., Iwamura, C., Hosokawa, H., Koseki, H., Tokoyoda, K., Suzuki, Y., Motohashi, S., and Nakayama, T.
  • Journal Title: Immunity Volume: 39 Issue: 5 Pages: 819-32
  • DOI: 10.1016/j.immuni.2013.09.012
  • Peer Reviewed
• [Journal Article] Gata3/Ruvbl2 complex regulates T helper 2 cell proliferation via repression of Cdkn2c expression. (2013)
  • Author(s): Hosokawa H, Tanaka T, Kato M, Tohyama H, Hanazawa A, Tamaki Y, Hirahara K, Sakikawa I, Morita A, Nagira M, Suzuki Y, Nakayama T.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 110(46) Issue: 46 Pages: 18626-18631
  • DOI: 10.1073/pnas.1311100110
  • Peer Reviewed
• [Journal Article] メモリーTh2細胞による慢性アレルギー性炎症の制御 (2013)
  • Author(s): 篠田健太、中山俊憲
  • Journal Title: BIO Clinica Volume: 28 Pages: 36-41
• [Journal Article] CD49b-dependent estabhshment of T helper cell memory. (2013)
  • Author(s): Hanazawa, A., Hayashizaki, K.,Shinoda, K., Yagita, H., Okumura, K., Löhning, M., Hara, T., Tani-ichi, S., Ikuta, K., Eckes, B.,Radbruch, A., Tokoyoda, K, and Nakayama, T.
  • Journal Title: Immuno1. Cell Bio1 Volume: 91 Issue: 8 Pages: 524-531
  • DOI: 10.1038/icb.2013.36
  • Peer Reviewed
• [Journal Article] 記憶ヘルパ－T細胞の形成と維持の分子機構 (2013)
  • Author(s): 篠田健太、中山俊憲
  • Journal Title: 臨床免疫・アレルギ－科 Volume: 59 Pages: 477-482
  • NAID: 40019682456
• [Presentation] Functionally distinct GATA3 complexes regulate Th2 cell differentiation and proliferation. (2014)
  • Author(s): Hosokawa, H., Shinoda, K., Suzuki, A., Ito, T., and Nakayama, T.
  • Organizer: 第43回日本免疫学会総会・学術集会
  • Place of Presentation: 国立京都国際会館(京都府京都市)
  • Year and Date: 2014-12-10 – 2014-12-12
• [Presentation] Bach2-Blimp1 axis plays an important role in the regulation of allergic airway inflammation. (2014)
  • Author(s): Izumoto, M., Kuwahara, M., Kiyoi, T., Shinoda, K., Nakayama, T., Kurosaki, T., and Yamashita, M.
  • Organizer: 第43回日本免疫学会総会・学術集会
  • Place of Presentation: 国立京都国際会館(京都府京都市)
  • Year and Date: 2014-12-10 – 2014-12-12
• [Presentation] The trithorax complex component Menin controls differentiation and maintenance of T helper 17 cells. (2014)
  • Author(s): Watanabe, Y., Onodera, A., Ichikawa, T., Obata-Ninomiya, K., Wada, T., Kiuchi, M., Morimoto, Y., Shinoda, K., Yagi, R., Motohashi, S., Hirahara, K., and Nakayama, T.
  • Organizer: 第43回日本免疫学会総会・学術集会
  • Place of Presentation: 国立京都国際会館(京都府京都市)
  • Year and Date: 2014-12-10 – 2014-12-12
• [Presentation] Induction of anti-tumor effect of BCG-LM through activation of eosinophils and effector memory Th2 cells.
  • Author(s): Ito, T., Hirahara, K., Suzuki, A., Shinoda, K., Hayashizaki, K., Wada, T., Yano, I., and Nakayama, T.
  • Organizer: 第42回日本免疫学会総会学術集会
  • Place of Presentation: 幕張メッセ（千葉県千葉市）
• [Presentation] Pathogenic memory Th2 cells in the airway and regulation by activated NKT cells in vivo.
  • Author(s): Nakayama, T., Iwamura, C., Shinoda, K., and Endo, Y.
  • Organizer: 第63回日本アレルギー学会秋季学術大会
  • Place of Presentation: ホテルニューオータニ（東京都千代田区）
• [Presentation] CD69 regulates the formation of resting T helper memory.
  • Author(s): Shinoda, K., and Nakayama, T.
  • Organizer: 7th International Leukocyte Signal Transduction Conference
  • Place of Presentation: Grecotel Conference Center Kos（Greece）
• [Presentation] The histone methyltransferase Ezh2 regulates differentiation and plasticity of CD4 T helper cells.
  • Author(s): Tumes, D., Onodera, A., Suzuki, A., Shinoda, K., Endo, Y., Iwamura, C., Hosokawa, H., Koseki, H., Tokoyoda, K., Suzuki, Y., and Nakayama, T.
  • Organizer: AAI Annual Meeting
  • Place of Presentation: Hawaii Convention Center (Honolulu, Hawaii, USA)
• [Remarks] 千葉大学大学院医学研究院免疫発生学
  • URL: http://www.m.chiba-u.ac.jp/class/meneki/