Regulation of early B cell development by the CCR4-NOT deadenylase complex

• Project/Area Number: 25860358
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Immunology
• Research Institution: Osaka University
• Principal Investigator: INOUE Takeshi 大阪大学, 免疫学フロンティア研究センター, 特任助教 (80466838)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: B細胞分化 / CCR4-NOT複合体 / mRNA分解 / p53 / mRNA代謝 / deadenylase / CCR4-NOT / CNOT3

Outline of Final Research Achievements

Post-transcriptional gene regulation is a key regulatory mechanism underlying mammalian cell differentiation and development. To elucidate physiological functions of the CCR4-NOT deadenylase complex, we analyzed the function of CNOT3 subunit of this complex using B cell development as a model system. We generated B-cell specific Cnot3-deficient mice and found that CNOT3 has an essential role for early B cell development. Furthermore, we identified a physiological target mRNA of this complex in early B cells.

Research Products

• [Presentation] Regulation of early B cell development by the CCR4-NOT mRNA deadenylase complex (2015)
  • Author(s): Takeshi Inoue
  • Organizer: Keystone Symposia
  • Place of Presentation: Fairmont Banff Springs, Canada
  • Year and Date: 2015-03-22 – 2015-03-27
• [Presentation] B細胞におけるCNOT3の機能解析 (2015)
  • Author(s): 井上 毅
  • Organizer: 第3回CCR4-NOT研究会
  • Place of Presentation: 東北大学
  • Year and Date: 2015-03-13
• [Presentation] B細胞分化・活性化におけるCNOT3の機能解析 (2014)
  • Author(s): 井上 毅
  • Organizer: 第2回CCR4-NOT研究会
  • Place of Presentation: 沖縄科学技術大学院大学
  • Year and Date: 2014-06-27 – 2014-06-29
• [Presentation] B細胞初期分化におけるCnot3の機能解析 (2013)
  • Author(s): 井上毅
  • Organizer: 第1回CCR4-NOT研究会
  • Place of Presentation: ホテルグランド天空(秋田県)
• [Presentation] B細胞分化におけるCNOT3の機能解析 (2013)
  • Author(s): 井上毅，黒崎知博
  • Organizer: 第5回シグナルネットワーク研究会
  • Place of Presentation: 北海道大学医学部
• [Presentation] B細胞分化におけるCCR4-NOT脱アデニル化酵素複合体サブユニットCNOT3の機能解析 (2013)
  • Author(s): 井上毅，森田斉弘，足達俊吾，夏目徹，深尾太郎，小原收，山本雅，黒崎知博
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（兵庫県）
• [Presentation] Essential role of CNOT3 subunit of the CCR4-NOT deadenylase complex during B cell development (2013)
  • Author(s): Takeshi Inoue，Tadashi Yamamoto，Tomohiro Kurosaki
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ（千葉県）