Development of nano/microbubbles for ultrasound mediated theranostics

• Project/Area Number: 25860397
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied pharmacology
• Research Institution: Teikyo University
• Principal Investigator: Oda Yusuke 帝京大学, 薬学部, 助手 (80505941)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: ナノ・マイクロバブル / 超音波 / セラノスティクス / 微小気泡 / マイクロバブル

Outline of Final Research Achievements

Development of nano/microbubbles for the ultrasound diagnostics and ultrasound mediated drug/gene delivery is important in order to establish effective ultrasound mediated theranostics. We find that the size of nano/microbubble is important factor to the stability and drug/gene delivery efficacy.

Research Products

• [Journal Article] Development of tissue-selectivegene delivery system with ultrasound (2016)
  • Author(s): Ryo Suzuki, Daiki Omata, Yusuke Oda, Mutsumi Sugii, Hitoshi Uruga, Hohan Unga, Yoichi Negishi, Sanae Oda, Kazuo Maruyama
  • Journal Title: Asian Journal of Pharmaceutical Sciences Volume: 2 Pages: 162-163
  • Peer Reviewed / Open Access
• [Journal Article] Development od cell-targeting bubble liposome for effective gene delivery with ultrasound (2016)
  • Author(s): Mutsumi Sugii, Ryo Suzuki, Yusuke Oda, Johan Unga, Daiki Omata, Hitoshi Uruga, Yoichi Negishi, Kazuo Martuyama
  • Journal Title: Asian Journal of Pharmaceutical Sciences Volume: 2 Issue: 1 Pages: 146-147
  • DOI: 10.1016/j.ajps.2015.11.083
  • Peer Reviewed / Open Access
• [Journal Article] Tumor growth suppression by the combination of nanobubbles and ultrasound (2016)
  • Author(s): Suzuki R, Oda Y, Omata D, Nishiie N, Koshima R, Shiono Y, Sawaguchi Y, Unga J,Naoi T, Negishi Y, Kawakami S, Hashida M, Maruyama K.
  • Journal Title: Cancer Sci. Volume: 107 Issue: 3 Pages: 217-113
  • DOI: 10.1111/cas.12867
  • NAID: 120005829401
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Development of fluorous lipid-based nanobubbles for efficiently containing perfluoropropane (2015)
  • Author(s): Yusuke Oda, Ryo Suzuki, Tatsuya Mori, Hideyo Takahashi, Hideaki Natsugari, Daiki Omata, Johan Unga, Hitoshi Uruga, Mutsumi Sugii, Shigeru Kawakami, Yuriko Higuchi, Fumiyoshi Yamashita, Mitsuru Hashida, Kazuo Maruyama
  • Journal Title: International Journal of Pharmaceutics Volume: 20 Issue: 1-2 Pages: 64-71
  • DOI: 10.1016/j.ijpharm.2015.03.073
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Nonviral gene delivery systems by the combination of bubble liposomes and ultrasound (2015)
  • Author(s): Daiki Omata, Yoichi Negishi, Ryo Suzuki, Yusuke Oda, Yoko Endo-Takahashi, Kazuo Maruyama
  • Journal Title: Advances in Genetics Volume: 89 Pages: 25-48
  • DOI: 10.1016/bs.adgen.2014.11.001
  • ISBN: 9780128022726
  • Peer Reviewed
• [Journal Article] Systemic delivery of miR-126 by miRNA-loaded Bubble liposomes for the treatment of hindlimb ischemia (2014)
  • Author(s): Endo-Takahashi Y, Negishi Y, Nakamura A, Ukai S, Ooaku K, Oda Y, Sugimoto K, Moriyasu F, Takagi N, Suzuki R, Maruyama K, Aramaki Y
  • Journal Title: Scientific Reports Volume: 4 Issue: 1 Pages: 3883-3883
  • DOI: 10.1038/srep03883
  • Peer Reviewed
• [Journal Article] バブルリポソームと超音波技術を利用した治療と診断 (2013)
  • Author(s): 丸山一雄、小俣大樹、鈴木 亮、小田雄介、澤口能一
  • Journal Title: ファインケミカル Volume: 42 Pages: 39-43
• [Journal Article] 超音波セラノスティックス (2013)
  • Author(s): 丸山一雄、小田雄介、小俣大樹、鈴木 亮
  • Journal Title: 薬剤学 Volume: 73 Pages: 194-202
  • NAID: 10031185372
• [Journal Article] Novel Strategies for Ultrasound Diagnostics and Therapeutics by Micro/Nanobubbles (2013)
  • Author(s): Ryo Suzuki, Yusuke Oda, Daiki Omata, Yoshikazu Sawaguchi, Mutsumi Seki, Hitoshi, Uruga, Tomoyuki Naoi, Yoichi Negishi, Kazuo Maruyama
  • Journal Title: Thermal Medicine Volume: 29 Issue: 2 Pages: 37-46
  • DOI: 10.3191/thermalmed.29.37
  • NAID: 10031183062
  • ISSN: 1882-2576, 1882-3750
  • Peer Reviewed
• [Journal Article] バブルリポソームと超音波を用いて抗原送達した樹状細胞の免疫によるがん転移予防効果 (2013)
  • Author(s): 小田雄介、鈴木 亮、関むつみ、小俣大樹、澤口能一、宇留賀仁史、丸山一雄
  • Journal Title: 超音波テクノ Volume: 25 Pages: 41-45
• [Presentation] Feasibility of Bubble liposomes as ultrasound contrast agent; in vivo animal study (2015)
  • Author(s): Yusuke Oda, Ryo Suzuki, Daiki Omata, Johan Unga, Hitoshi Uruga, Mutsumi Sugii, Kazuo Maruyama
  • Organizer: World Molecular Imaging Congress
  • Place of Presentation: Honolulu, Hawaii
  • Year and Date: 2015-09-02
  • Int'l Joint Research
• [Presentation] フッ化リン脂質を利用したバブルリポソームの開発 (2015)
  • Author(s): 小田雄介、高橋秀依、鈴木 亮、小俣大樹、Unga Johan、宇留賀仁史、杉井むつみ、川上 茂、樋口ゆり子、橋田 充、丸山一雄
  • Organizer: 日本薬学会
  • Place of Presentation: 神戸学院大学
  • Year and Date: 2015-03-25 – 2015-03-28
• [Presentation] 微小気泡の粒子径が及ぼす超音波遺伝子導入への影響 (2014)
  • Author(s): 三瓶芳莉、小田雄介、鈴木 亮、石川 舞、小俣大樹、Johan Unga、宇留賀仁史、関むつみ、根岸洋一、丸山一雄
  • Organizer: 日本DDS学会
  • Place of Presentation: 慶應義塾大学薬学部芝共立キャンパス
  • Year and Date: 2014-07-30 – 2014-07-31
• [Presentation] ソノポレーションを駆使した樹状細胞への抗原送達法の開発とがん免疫療法への応用 (2014)
  • Author(s): 小田雄介、鈴木 亮、小俣大樹、Johan Unga、丸山一雄
  • Organizer: 日本超音波医学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-05-09 – 2014-05-11
  • Invited
• [Presentation] 微小気泡の粒子径が及ぼす超音波造影能と超音波遺伝子導入効率への影響 (2014)
  • Author(s): 石川 舞、小田雄介、鈴木 亮、三瓶芳莉、宇留賀仁史、小俣大樹、澤口能一、関むつみ、丸山一雄
  • Organizer: 日本薬学会 第134年会
  • Place of Presentation: 熊本市総合体育館
• [Presentation] バブルリポソームの安定性におよぼす脂質組成の影響 (2013)
  • Author(s): 小田雄介、鈴木 亮、小俣大樹、澤口能一、宇留賀仁史、関むつみ、根岸洋一、川上 茂、樋口ゆり子、橋田 充、丸山一雄
  • Organizer: 日本DDS学会
  • Place of Presentation: 京都テレサ
• [Presentation] 脂質組成の異なるバブルリポソームの特性評価 (2013)
  • Author(s): 小田雄介、鈴木 亮、宇留賀仁史、小俣大樹、澤口能一、関むつみ、根岸洋一、丸山一雄
  • Organizer: 第12回 日本超音波治療研究会
  • Place of Presentation: 帝京大学 板橋キャンパス
• [Presentation] 超音波造影と超音波遺伝子導入に対する微小気泡の粒子径の影響 (2013)
  • Author(s): 石川 舞、小田雄介、鈴木 亮、三瓶芳莉、宇留賀仁史、小俣大樹、澤口能一、関むつみ、丸山一雄
  • Organizer: 第12回 日本超音波治療研究会
  • Place of Presentation: 帝京大学 板橋キャンパス
• [Presentation] Brain Specific Gene Delivery by Bubble liposomes and Ultrasound (2013)
  • Author(s): Y. Oda, R. Suzuki, D. Omata, Y. Sawaguchi, Y. Negishi, K. Maruyama
  • Organizer: 40th Annual Meeting & Exposition of the Controlled Release Society
  • Place of Presentation: Hawaii Convention Center
• [Remarks] 帝京大学薬学部薬物送達学研究室
  • URL: http://www.teikyo-dds-lab.com/
• [Remarks] 帝京大学薬学部薬物送達学研究室（DDS研）
  • URL: http://www.teikyo-dds-lab.com/
• [Remarks] 帝京大学 薬学部 薬物送達学研究室
  • URL: http://www.teikyo-dds-lab.com/