Real-time imaging of cell penetrating peptide (CPP) in the cytosol
| Project/Area Number |
25860400
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied pharmacology
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| Research Institution | Niigata University (2014-2016)
Osaka Ohtani University (2013) |
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Principal Investigator |
Okuda Akiko 新潟大学, 医歯学系, 助教 (60454584)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | 膜透過ペプチド / ドラッグデリバリー / エンドサイトーシス / CPP |
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| Outline of Final Research Achievements |
Cell-penetrating peptides (CPPs) can enter cells with cargo. However, endocytosis plays a major role in their uptake and keep most of CPP-cargo trapped in endosomes. In this study, I designed peptides which could green fluorescent protein (EGFP) and immunoglobulin (IgG) into cytosol of HEK293. Furthermore, it was indicated that cellular uptake of CPP-EGFP and cytosolic delivery occur via endocytosis.
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Report
(5 results)
Research Products
(3 results)
