Real-time imaging of cell penetrating peptide (CPP) in the cytosol

Research Project

Project/Area Number	25860400
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Applied pharmacology
Research Institution	Niigata University (2014-2016) Osaka Ohtani University (2013)
Principal Investigator	Okuda Akiko 新潟大学, 医歯学系, 助教 (60454584)
Project Period (FY)	2013-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000) Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords	膜透過ペプチド / ドラッグデリバリー / エンドサイトーシス / CPP
Outline of Final Research Achievements	Cell-penetrating peptides (CPPs) can enter cells with cargo. However, endocytosis plays a major role in their uptake and keep most of CPP-cargo trapped in endosomes. In this study, I designed peptides which could green fluorescent protein (EGFP) and immunoglobulin (IgG) into cytosol of HEK293. Furthermore, it was indicated that cellular uptake of CPP-EGFP and cytosolic delivery occur via endocytosis.

Report

(5 results)