| Project/Area Number |
25860413
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Bunkyo Gakuin University |
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Principal Investigator |
AKI Nakayama 文京学院大学, 保健医療学部, 助手 (00599425)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | エキソソーム / 尿タンパク質 / バイオマーカー / 腎機能 / エクソソーム / プロテオーム / 免疫電子顕微鏡法 / 尿中エキソソーム / 二次元電気泳動法 / 質量分析法 / Tamm-Horsfall protein |
|---|
| Outline of Final Research Achievements |
We identified urinary exosomal proteins by two dimensional electrophoresis-based proteome analyses which include membranous, extracellular region, and cytoplasm of renal epithelial cells. The numbers, diameters, and concentration of protein of exosomal fraction were consistent between healthy subjects. Furthermore, total protein concentration of urinary exosome fraction was consistent per unit time. This research provides the basic information about protein profile and amount of exosomal production in renal epithelial cell of healthy subjects.
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