In vivo patch-clamp analysis of itch transmission in central nerve system

• Project/Area Number: 25860431
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pain science
• Research Institution: University of Toyama (2014); National Institute for Physiological Sciences (2013)
• Principal Investigator: UTA Daisuke 富山大学, 大学院医学薬学研究部(薬学), 助教 (70598416)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 痒み / 脊髄後角 / in vivoパッチクランプ / 5-ＨＴ / 痛み / 大脳皮質 / シナプス伝達 / 中枢神経 / セロトニン / c-Fos / TRP / in vivo patch clamp / 脊髄 / 膠様質ニューロン

Outline of Final Research Achievements

The mechanism for the itch transmission in the central nervous system remains unclear. The present study was designed to analyze pruritic synaptic responses evoked in spinal dorsal horn and cortex neurons of rodents by using an in vivo whole-cell patch-clamp and extracellular recording technique. The present main finding is that subpopulation of spinal dorsal horn and cortex neurons are responsible for pruritic cutaneous 5-HT stimulation.

Research Products

• [Journal Article] Three-dimensional distribution of sensory stimulation-evoked neuronal activity of spinal dorsal horn neurons analyzed by in vivo calcium imaging (2014)
  • Author(s): Nishida K, Matsumura S, Taniguchi W, Uta D, Furue H, Ito S.
  • Journal Title: PLoS One Volume: 9(8) Issue: 8 Pages: e103321-e103321
  • DOI: 10.1371/journal.pone.0103321
  • Peer Reviewed / Open Access
• [Journal Article] Firing pattern of substantia gelatinosa neurons receiving TRPA1-expressing afferents in rat spinal cord. (2014)
  • Author(s): Uta D, Yoshimura M, Imoto K and Furue H.
  • Journal Title: The Journal of Functional Diagnosis of the Spinal Cord Volume: 35 Pages: 3-9
  • NAID: 40020382915
  • Peer Reviewed
• [Journal Article] Systemic dexmedetomidine augments inhibitory synaptic transmission in the superficial dorsal horn through activation of descending noradrenergic control : an in vivo patch-clamp analysis of analgesic mechamisms (2014)
  • Author(s): Funai Y, Pickering AE, Uta D, Nishikawa K, Mori T, Asada A, Imoto K and Furue H
  • Journal Title: Pain Volume: 155(3) Issue: 3 Pages: 617-628
  • DOI: 10.1016/j.pain.2013.12.018
  • Peer Reviewed
• [Journal Article] 脊髄における侵害受容機構（Neuronal mechanisms of spinal nociception） (2014)
  • Author(s): 古江秀昌、歌 大介、秋元 望
  • Journal Title: 月刊ファインケミカル Volume: 43 Pages: 17-21
• [Journal Article] CCL-1 in the spinal cord contributes to neuropathic pain induced by nerve injury. (2013)
  • Author(s): Nozomi Akimoto, Kenji Honda, Daisuke Uta, Kaoru Beppu, Yuichi Ushijima, Yu Matsuzaki, Shigeto Nakashima, Mizuho A. Kido, Keiji lmoto, Yukio Takano & Mami Noda
  • Journal Title: Cell Death & Disease Volume: 4 Issue: 6 Pages: e679-e679
  • DOI: 10.1038/cddis.2013.198
  • Peer Reviewed
• [Presentation] Three dimensional reconstruction of trigeminal ganglion cell processes labeled by intracellular injection: Emphasis on club-like endings. (2015)
  • Author(s): Tonomura S, Ebara S, Uta D, Furue H, Furuta T, Kuroda D and Kumamoto K.
  • Organizer: Proceedings of 120th Annual Meeting of the Japanese Association of Anatomists and the 92nd Auual Meeting of the Physiological Society of Japan
  • Place of Presentation: 神戸コンベンションセンター
  • Year and Date: 2015-03-23
• [Presentation] The role of TRP channel related to thermal effect during carbon dioxide-rich water immersion. (2015)
  • Author(s): Nishimura N, Iwase S, Nishimura R, Uta D, Matsumoto T and Sato M．
  • Organizer: Proceedings of 120th Annual Meeting of the Japanese Association of Anatomists and the 92nd Auual Meeting of the Physiological Society of Japan
  • Place of Presentation: 神戸コンベンションセンター
  • Year and Date: 2015-03-22
• [Presentation] Simultaneous observation of skin receptors and central terminations on single primary sensory neuron. (2015)
  • Author(s): Ebara S, Tonomura S, Uta D, Furue H, Furuta T, Kuroda D and Kumamoto K.
  • Organizer: Proceedings of 120th Annual Meeting of the Japanese Association of Anatomists and the 92nd Auual Meeting of the Physiological Society of Japan
  • Place of Presentation: 神戸コンベンションセンター
  • Year and Date: 2015-03-21
• [Presentation] Selective excitatory effect of TRPA1 agonists on synaptic transmission in superficial spinal dorsal horn neurons of the adult rat. (2015)
  • Author(s): Uta D, Imoto K and Furue H.
  • Organizer: The 88th Annual Meeting of the Japan Pharmacological Society
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2015-03-20
• [Presentation] 成熟ラット脊髄後角膠様質ニューロンへの後根刺激誘起のシナプス伝達に対するTRPA1作動薬の応答。 (2015)
  • Author(s): 歌 大介、吉村 恵、井本 敬二、古江 秀昌.
  • Organizer: 第36回脊髄機能診断研究会
  • Place of Presentation: イイノホール＆カンファレンスセンター
  • Year and Date: 2015-02-07
• [Presentation] In vivoパッチクランプ法をもちいた成熟ラット脊髄後角における痒み情報伝達機構の解析。 (2015)
  • Author(s): 歌 大介.
  • Organizer: 脊髄運動・感覚統合研究会
  • Place of Presentation: 熊本保健科学大学
  • Year and Date: 2015-01-09
• [Presentation] 一次感覚神経におけるTRPV1-ANO1相互作用による疼痛メカニズム。 (2014)
  • Author(s): 高山 靖規、歌 大介、古江 秀昌、富永 真琴.
  • Organizer: 「痛みと痛覚情動連関の神経機構」平成26年度生理研研究会
  • Place of Presentation: 岡崎カンファレンスセンター
  • Year and Date: 2014-12-10
• [Presentation] A pain-enhancing mechanism through TRPV1-ANO1 interaction, and its inhibition effect by drag. (2014)
  • Author(s): Takayama Y, Uta D, Furue H and Tominaga M.
  • Organizer: The 41th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: Walter E. Washington Convention Center, Washington D.C., USA
  • Year and Date: 2014-11-16
• [Presentation] In vivo spinal excitatory synaptic responses and scratching behaviors evoked by cutaneous 5-HT application. (2014)
  • Author(s): Uta D, Andoh T, Kuraishi Y, Imoto K and Furue H.
  • Organizer: The 24th International Symposium of Itch
  • Place of Presentation: Belle Salle Jimbocho
  • Year and Date: 2014-10-18
• [Presentation] Three dimensional reconstruction of trigeminal ganglion cell processes labeled by intracellular injection: Emphasis on club-like endings. (2014)
  • Author(s): Tonomura S, Ebara S, Meir I, Bagdasarian K, Kuroda D, Uta D, Furue H, Furuta T, Ahissar E and Kumamoto K.
  • Organizer: The 37th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-09-13
• [Presentation] Electrophysiological analysis of spinal dorsal horn neurons receiving pruriceptive afferents in the adult rat spinal cord. (2014)
  • Author(s): Uta D, Andoh T, Kuraishi Y, Imoto K and Furue H.
  • Organizer: The 37th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-09-12
• [Presentation] Enhancement of TRPV1-mediated nociception by anoctamin 1 activation. (2014)
  • Author(s): Takayama Y, Uta D, Furue H and Tominaga M.
  • Organizer: The 37th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-09-12
• [Presentation] Action of Chemokine (C-C motif) ligand 1 on synaptic transmission in the spinal superficial dorsal horn. (2014)
  • Author(s): Akimoto N, Uta D, Honda K, Takano Y, Imoto K, Noda M and Furue H.
  • Organizer: 2014 NIPS-KU/YU Symposium
  • Place of Presentation: Okazaki Conference center
  • Year and Date: 2014-07-02
• [Presentation] A pain-enhancing mechanism involving the interaction between TRPV1 and anoctamin 1. (2014)
  • Author(s): Takayama Y, Uta D, Furue H and Tominaga M.
  • Organizer: 第36回日本疼痛学会
  • Place of Presentation: KKRホテル大阪
  • Year and Date: 2014-06-21
• [Presentation] Effect of TRPA1 and TRPV1 agonists on synaptic transmission evoked in morphologically distinct cell types in the substantia gelatinosa of the adult rat spinal cord. (2014)
  • Author(s): Uta D, Imoto K and Furue H.
  • Organizer: 第36回日本疼痛学会
  • Place of Presentation: KKRホテル大阪
  • Year and Date: 2014-06-20
• [Presentation] Action of CCL-1 on synaptic transmission in the spinal dorsal horn. (2014)
  • Author(s): Akimoto N, Uta D, Honda K, Takano Y, Imoto K, Noda M and Furue H.
  • Organizer: 第36回日本疼痛学会
  • Place of Presentation: KKRホテル大阪
  • Year and Date: 2014-06-20
• [Presentation] In vivoパッチクランプ記録法を用いた脊髄後角における痒み情報伝達の解析
  • Author(s): 歌 大介、秋元 望、井本敬二、古江秀昌
  • Organizer: 第15回ブレインサイエンス研究会
  • Place of Presentation: 休暇村志賀島（福岡県）
• [Presentation] Firing pattern of spinal dorsal horn neurons receiving pruritogen serotonin-responsive afferents in the rat spinal cord
  • Author(s): Uta D, Andoh T, Kuraishi Y, Imoto K and Furue H
  • Organizer: The 36rd Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 国立京都国際会議場（京都府）
• [Presentation] Selective use-dependent inhibition of levobupivacaine on TTX resistant (NaV1.8) sodium currents
  • Author(s): Kawatsu R, Imoto K, Uta D, Okamoto T, Taya K, Yamamura Y, Ikeda M and Furue H
  • Organizer: The 36rd Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 国立京都国際会議場（京都府）
• [Presentation] Full visualization of single trigeminal ganglion neuron
  • Author(s): 54. Ebara S, Tonomura S, Kumamoto K, Kuroda D, Furuta T, Furue H and Uta D
  • Organizer: The 36rd Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 国立京都国際会議場（京都府）
• [Presentation] In vivo patch-clamp analysis of local anesthetic actions on synaptic transmission to the adult rat spinal dorsal horn
  • Author(s): Uta D, Imoto K and Furue H
  • Organizer: 第35回日本疼痛学会
  • Place of Presentation: 大宮ソニックシティ（埼玉県）
• [Presentation] Inhibitory action of local anesthetic, levobupivacaine on NaV1.8 channel
  • Author(s): Kawatsu R, Imoto K, Uta D, Taya K, Yamamura Y and Furue H
  • Organizer: 第35回日本疼痛学会
  • Place of Presentation: 大宮ソニックシティ（埼玉県）
• [Presentation] A new target in the treatment for neuropathic pain induced by nerve injury
  • Author(s): Noda M, Akimoto N, Honda K, Uta D, Furue H, Kido M, Imoto K, Takano Y
  • Organizer: The 11th Korea-Japan Joint Symposium of Brain Sciences, and Cardiac and Smooth Muscle Sciences
  • Place of Presentation: Act City Hamamatsu Congress center（Sizuoka）
• [Presentation] In vivo patch-clamp recording analysis of excitatory synaptic transmission in the adult rat spinal dorsal horn evoked by cutaneous application of a pruritogen
  • Author(s): Uta D, Andoh T, Kuraishi Y, Imoto K and Furue H
  • Organizer: The 23th International Symposium of Itch
  • Place of Presentation: Senri Life Science center(Osaka)
  • Invited
• [Presentation] In vivoパッチクランプ法を用いた脊髄後角における痒み情報伝達機構の解析
  • Author(s): 歌 大介
  • Organizer: 日本薬学会北陸支部特別講演会
  • Place of Presentation: 富山大学薬学部研究棟II（富山県）
  • Invited
• [Presentation] Firing pattern and morphological analysis of substantia gelatinosa neurons receiving TRPA1-expressing afferent fibers in adult rat spinal dorsal horn
  • Author(s): Uta D, Imoto K and Furue H
  • Organizer: The 5th Asian Pain Symposium
  • Place of Presentation: Okazaki Conference center(Aichi)
• [Presentation] Effect of Chemokine (C-C motif) Ligand 1 on Synaptic Transmission in the Spinal Dorsal Horn.
  • Author(s): Akimoto N, Uta D, Honda K, Takano Y, Imoto K, Noda M and Furue H
  • Organizer: The 5th Asian Pain Symposium
  • Place of Presentation: Okazaki Conference center(Aichi)
• [Presentation] TRPA1発現線維が入力するラット脊髄後角膠様質細胞の発火パターン解析
  • Author(s): 歌 大介、吉村 恵、井本敬二、古江秀昌
  • Organizer: 第35回脊髄機能診断研究会
  • Place of Presentation: 東京商工会議所ビル国際会議場（東京都）
• [Presentation] In vivoパッチクランプ法による感覚シナプス伝達機構の解析
  • Author(s): 歌 大介
  • Organizer: 第3回新潟脳研―生理研 合同シンポジウム
  • Place of Presentation: 岡崎カンファレンスセンター（愛知県）
• [Presentation] The role of TRPV1 that contribute to the thermal effects of immersion to the carbon dioxide-rich water
  • Author(s): Nishimura N, Iwase S, Nishimura R, Uta D, Matsumoto T and Sato M
  • Organizer: The 91th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: 鹿児島大学郡元キャンパス（鹿児島県）
• [Presentation] Spinal neuronal responses elicited by intradermal pruritogen injection in hairless mice
  • Author(s): Ueda Y, Ishii R, Uta D, Imoto K and Furue H
  • Organizer: The 91th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: 鹿児島大学郡元キャンパス（鹿児島県）
• [Presentation] Reduction of TRPV1-mediated pain sensation by TMEM16A inhibition
  • Author(s): Takayama Y, Uta D and Tominaga M
  • Organizer: The 91th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: 鹿児島大学郡元キャンパス（鹿児島県）
• [Presentation] Effect of CCL-1 on synaptic transmission in the spinal superficial dorsal horn
  • Author(s): Akimoto N, Uta D, Honda K, Takano Y, Imoto K, Noda M and Furue H
  • Organizer: The 91th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: 鹿児島大学郡元キャンパス（鹿児島県）
• [Presentation] Firing pattern of spinal dorsal horn neurons receiving pruriceptive afferents in the adult rat spinal cord
  • Author(s): Uta D, Andoh T, Kuraishi Y, Imoto K and Furue H
  • Organizer: The 91th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: 鹿児島大学郡元キャンパス（鹿児島県）
• [Remarks] 富山大学医学薬学研究部応用薬理学研究室ホームページ
  • URL: http://www.pha.u-toyama.ac.jp/phapha2/Japanese/frame.htm
• [Remarks] 生理学研究所生体情報研究系神経シグナル研究部門ホームページ
  • URL: http://www.nips.ac.jp/huinfo/