| Project/Area Number |
25860466
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hygiene and public health
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
YASUDA SHUSUKE 京都府立医科大学, 医学(系)研究科(研究院), 研究員 (10643398)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 悪性腫瘍 / TRAIL / 癌予防 / アポトーシス |
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| Outline of Final Research Achievements |
Sulforaphane enhances apoptosis induced by Lactobacillus-stimulated PBMC in human colon cancer HCT116 cells and HT-29 cells. The apoptosis was considerably blocked by the pan-caspase inhibitor zVAD-fmk, which indicated that apoptosis was caspase-dependent in both colon cancer cells. Furthermore, TNFR/Fc chimera significantly at least partially inhibited the apoptosis in both colon cancer cells.
The expression of cIAP-2 was significantly up-regulated by the co-culture with Lactobacillus-stimulated PBMC in both colon cancer cells. Interestingly, sulforaphane treatment suppressed the induction of cIAP-2 by the co-culture, and restored the cIAP-2 expression to the normal level almost. These results showed the relationship of TNFα and cIAP-2 in the apoptosis by the combination.
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