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Micro RNA profiles in pancreatic cancer

Research Project

Project/Area Number 25860514
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Gastroenterology
Research InstitutionHokkaido University

Principal Investigator

KAWAKUBO Kazumichi  北海道大学, 医学(系)研究科(研究院), 助教 (80633578)

Co-Investigator(Renkei-kenkyūsha) NATSUIZAKA Mitsuteru  北海道大学, 北海道大学病院, 助教 (80642446)
KUBOTA Yoshimasa  北海道大学, 大学院医学研究科
KAWAKAMI Hiroshi  北海道大学, 北海道大学病院, 助教 (80399823)
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords遺伝子変異解析 / EUS-FNA / 膵癌 / 遺伝子網羅的解析
Outline of Final Research Achievements

DNA was extracted from 38 pancreatic tumors and CTNNB1 gene mutation was analyzed by next generation sequencer. Seven out of 7 solid-pseudopapillary neoplasm showed a single base pair missence mutations in exon 3 of CTNNB1 with variant frequency of 5.4 to 48.8%.
DNA was extracted from cyst fluid or pancreatic juice in 5 patients with intraductal papillary mucinous neoplasms. We analyzed 36 cancer related gene mutations by next generation sequencer. Four GNAS and 2 KRAS mutations were detected with variant frequency of 2-29.8 and 6-21% respectively.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (23 results)

All 2015 2014

All Journal Article (18 results) (of which Peer Reviewed: 18 results,  Open Access: 4 results) Presentation (3 results) Book (2 results)

  • [Journal Article] Hepatobiliary and Pancreatic: A splenic artery aneurysm presenting as a calcified pancreatic mass.2015

    • Author(s)
      Kawakubo K, Kawakami H, Kuwatani M, Sakamoto N.
    • Journal Title

      J Gastroenterol Hepatol.

      Volume: 30 Issue: 4 Pages: 655-655

    • DOI

      10.1111/jgh.12848

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Technical tips and troubleshooting of endoscopic biliary drainage for unresectable malignant hilar biliary obstruction.2015

    • Author(s)
      Kawakami H, Itoi T, Kuwatani M, Kawakubo K, Kubota Y, Sakamoto N.
    • Journal Title

      J Hepatobiliary Pancreat Sci.

      Volume: 22 Issue: 4

    • DOI

      10.1002/jhbp.186

    • NAID

      120005740324

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A unique use of a double-pigtail plastic stent: correction of kinking of the common bile duct due to a metal stent.2015

    • Author(s)
      Kuwatani M, Kawakami H, Abe Y, Kawahata S, Kawakubo K, Kubo K, Sakamoto N.
    • Journal Title

      Gut Liver.

      Volume: 9 Issue: 2 Pages: 251-252

    • DOI

      10.5009/gnl14286

    • NAID

      120005600753

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Incidental Pancreatic Cyst is a Significant Predictor of Mortality? What Determines Life Expectancy?2015

    • Author(s)
      Kawakubo K, Kawakami H, Kuwatani M, Sakamoto N.
    • Journal Title

      Radiology.

      Volume: 274 Issue: 3 Pages: 939-940

    • DOI

      10.1148/radiol.14141965

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Single-step simultaneous side-by-side placement of a self-expandable metallic stent with a 6-Fr delivery system for unresectable malignant hilar biliary obstruction: a feasibility study.2015

    • Author(s)
      Kawakubo K, Kawakami H, Kuwatani M, Kudo T, Abe Y, Kawahata S, Kubo K, Kubota Y, Sakamoto N.
    • Journal Title

      J Hepatobiliary Pancreat Sci.

      Volume: 22 Issue: 2 Pages: 151-155

    • DOI

      10.1002/jhbp.173

    • NAID

      40020363680

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Risk factors for technical failure of endoscopic double self-expandable metallic stent placement by partial stent-in-stent method.2015

    • Author(s)
      Kawakubo K, Kawakami H, Toyokawa Y, Otani K, Kuwatani M, Abe Y, Kawahata S, Kubo K, Kubota Y, Sakamoto N.
    • Journal Title

      J Hepatobiliary Pancreat Sci.

      Volume: 22 Issue: 1 Pages: 79-85

    • DOI

      10.1002/jhbp.170

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CTNNB1 mutational analysis of solid-pseudopapillary neoplasms of the pancreas using endoscopic ultrasound-guided fine-needle aspiration and next-generation deep sequencing.2014

    • Author(s)
      Kubota Y, Kawakami H, Natsuizaka M, Kawakubo K, Marukawa K, Kudo T, Abe Y, Kubo K, Kuwatani M, Hatanaka Y, Mitsuhashi T, Matsuno Y, Sakamoto N.
    • Journal Title

      J Gastroenterol.

      Volume: in press Issue: 2 Pages: 203-210

    • DOI

      10.1007/s00535-014-0954-y

    • NAID

      120005693897

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High and low negative pressure suction techniques in EUS-guided fine-needle tissue acquisition by using 25-gauge needles: A multicenter, prospective, randomized, controlled trial2014

    • Author(s)
      Kudo T, Kawakami H, Hayashi T, Yasuda I, Mukai T, Inoue H, Katanuma A, Kawakubo K, Ishiwatari H, Doi S, Yamada R, Maguchi H, Isayama H, Mitsuhashi T, Sakamoto N
    • Journal Title

      Gastrointest Endosc.

      Volume: 80 Issue: 6 Pages: 1030

    • DOI

      10.1016/j.gie.2014.04.012

    • NAID

      120005539217

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Difference from bile duct cancer and relationship between bile duct wall thickness and serum IgG/IgG4 levels in IgG4-related sclerosing cholangitis.2014

    • Author(s)
      Kuwatani M, Kawakami H, Zen Y, Kawakubo K, Kudo T, Abe Y, Kubo K, Sakamoto N.
    • Journal Title

      Hepatogastroenterology.

      Volume: 61 Pages: 1852-1856

    • NAID

      120005672362

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intravenous and intraperitoneal paclitaxel with S-1 for refractory pancreatic cancer with malignant ascites: an interim analysis.2014

    • Author(s)
      Takahara N, Isayama H, Nakai Y, Sasaki T, Ishigami H, Yamashita H, Yamaguchi H, Hamada T, Uchino R, Mizuno S, Miyabayashi K, Mohri D, Kawakubo K, Kogure H, Yamamoto N, Sasahira N, Hirano K, Ijichi H, Tateishi K, Tada M, Kitayama J, Watanabe T, Koike K.
    • Journal Title

      J Gastrointest Cancer.

      Volume: 45(3) Issue: 3 Pages: 307-11

    • DOI

      10.1007/s12029-014-9603-1

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Transmural biliary drainage can be an alternative to transpapillary drainage in patients with an indwelling duodenal stent.2014

    • Author(s)
      Hamada T, Isayama H, Nakai Y, Kogure H, Yamamoto N, Kawakubo K, Takahara N, Uchino R, Mizuno S, Sasaki T, Togawa O, Matsubara S, Ito Y, Hirano K, Tsujino T, Tada M, Koike K.
    • Journal Title

      Dig Dis Sci.

      Volume: 59 Issue: 8 Pages: 1931-8

    • DOI

      10.1007/s10620-014-3062-1

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Slow pull versus suction in endoscopic ultrasound-guided fine-needle aspiration of pancreatic solid masses.2014

    • Author(s)
      Nakai Y, Isayama H, Chang KJ, Yamamoto N, Hamada T, Uchino R, Mizuno S, Miyabayashi K, Yamamoto K, Kawakubo K, Kogure H, Sasaki T, Hirano K, Tanaka M, Tada M, Fukayama M, Koike K.
    • Journal Title

      Dig Dis Sci.

      Volume: 59 Issue: 7 Pages: 1578-85

    • DOI

      10.1007/s10620-013-3019-9

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Safety and utility of single-session endoscopic ultrasonography and endoscopic retrograde cholangiopancreatography for the evaluation of pancreatobiliary diseases.2014

    • Author(s)
      Kawakubo K, Kawakami H, Kuwatani M, Haba S, Kudo T, Abe Y, Kawahata S, Onodera M, Ehira N, Yamato H, Eto K, Sakamoto N.
    • Journal Title

      Gut Liver.

      Volume: 8 Issue: 3 Pages: 329-332

    • DOI

      10.5009/gnl.2014.8.3.329

    • NAID

      120005455894

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Multicenter retrospective study of endoscopic ultrasound-guided biliary drainage for malignant biliary obstruction in Japan2014

    • Author(s)
      Kawakubo K, Isayama H, Kato H, Itoi T, Kawakami H, Hanada K, Ishiwatari H, Yasuda I, Kawamoto H, Itokawa F, Kuwatani M, Iiboshi T, Hayashi T, Doi S, Nakai Y.
    • Journal Title

      J Hepatobiliary Pancreat Sci.

      Volume: 21 Issue: 5 Pages: 328

    • DOI

      10.1002/jhbp.27

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Influence of the safety and diagnostic accuracy of preoperative endoscopic ultrasound-guided fine-needle aspiration for resectable pancreatic cancer on clinical performance.2014

    • Author(s)
      Kudo T, Kawakami H, Kuwatani M, Eto K, Kawahata S, Abe Y, Onodera M, Ehira N, Yamato H, Haba S, Kawakubo K, Sakamoto N.
    • Journal Title

      World J Gastroenterol.

      Volume: 20 Issue: 13 Pages: 3620-3627

    • DOI

      10.3748/wjg.v20.i13.3620

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Acute pancreatitis caused by Anisakis.2014

    • Author(s)
      Yamato H, Kawakami H, Takagi K, Ogawa K, Hatanaka K, Yamamoto Y, Naruse H, Kawakubo K, Sakamoto N.
    • Journal Title

      Gastrointest Endosc.

      Volume: 79 Issue: 4 Pages: 676-676

    • DOI

      10.1016/j.gie.2013.11.026

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Is intraductal papillary mucinous neoplasm of the pancreas just a precursor or identical with pancreatic ductal adenocarcinoma?2014

    • Author(s)
      Kawakubo K, Kawakami H, Kuwatani M, Sakamoto N.
    • Journal Title

      Am J Gastroenterol.

      Volume: 109 Issue: 3 Pages: 446-447

    • DOI

      10.1038/ajg.2013.468

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Disease-Specific Mortality Among Patients With Intraductal Papillary Mucinous Neoplasm of the Pancreas2014

    • Author(s)
      Kawakubo K, Tada M, Isayama H, Sasahira N, Nakai Y, Takahara N, Uchino R, Hamada T, Miyabayashi K, Yamamoto K, Mizuno S, Mohri D, Kogure H, Sasaki T, Yamamoto N, Hirano K, Ijichi H, Tateishi K, Koike K
    • Journal Title

      Clin Gastroenterol Hepatol

      Volume: 12(3) (2013 Jul 25) Issue: 3 Pages: 486-91

    • DOI

      10.1016/j.cgh.2013.06.032

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Presentation] EUS-guided choledochoduodenostomy with a conventional metallic stent: outcome of a single center experience2014

    • Author(s)
      Kawakubo K, Kawakami, H, Kuwatani, M, Sakamoto, N
    • Organizer
      SGI2014
    • Place of Presentation
      ソウル(大韓民国)
    • Year and Date
      2014-10-25 – 2014-10-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] Feasibility of single-step simultaneous side-by-side placement using 6Fr self-expandable metallic stent2014

    • Author(s)
      Kawakubo K, Kawakami H, Kuwatani M, Abe Y, Kawahata S, Kubota Y, Kubo K, Sakamoto N
    • Organizer
      DDW2014
    • Place of Presentation
      シカゴ(米国)
    • Year and Date
      2014-05-03 – 2014-05-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] Risk factors for technical failure of endoscopic multiple metallic stent placement by stent-in-stent method for unresectable malignant hilar biliary obstruction2014

    • Author(s)
      Kawakubo K, Kawakami H, Kuwatani M, Abe Y, Kawahata S, Kubota Y, Kubo K and Sakamoto N
    • Organizer
      DDW2014
    • Place of Presentation
      シカゴ(米国)
    • Year and Date
      2014-05-03 – 2014-05-06
    • Related Report
      2014 Annual Research Report
  • [Book] 【消化器疾患に対する内視鏡的アプローチの最新情報】 胆道疾患に対するInterventional EUSの現況と展望.2014

    • Author(s)
      川久保和道, 河上洋, 桑谷将城, 羽場真, 阿部容子, 川畑修平, 久保田良政, 工藤大樹, 久保公利, 坂本直哉.
    • Total Pages
      7
    • Publisher
      映像情報Medical
    • Related Report
      2014 Annual Research Report
  • [Book] 【患者にやさしいERCPの工夫】 Wire-guided cannulation(WGC)により胆管挿管率の向上とERCP後膵炎の合併の低下は得られるのか?2014

    • Author(s)
      河上洋, 桑谷将城, 川久保和道, 工藤大樹, 阿部容子, 久保公利, 久保田良政, 坂本直哉.
    • Total Pages
      8
    • Publisher
      消化器内科
    • Related Report
      2014 Annual Research Report

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Published: 2014-07-25   Modified: 2019-07-29  

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