Micro RNA profiles in pancreatic cancer

• Project/Area Number: 25860514
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Hokkaido University
• Principal Investigator: KAWAKUBO Kazumichi 北海道大学, 医学(系)研究科(研究院), 助教 (80633578)
• Co-Investigator(Renkei-kenkyūsha): NATSUIZAKA Mitsuteru 北海道大学, 北海道大学病院, 助教 (80642446) ; KUBOTA Yoshimasa 北海道大学, 大学院医学研究科 ; KAWAKAMI Hiroshi 北海道大学, 北海道大学病院, 助教 (80399823)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 遺伝子変異解析 / EUS-FNA / 膵癌 / 遺伝子網羅的解析

Outline of Final Research Achievements

DNA was extracted from 38 pancreatic tumors and CTNNB1 gene mutation was analyzed by next generation sequencer. Seven out of 7 solid-pseudopapillary neoplasm showed a single base pair missence mutations in exon 3 of CTNNB1 with variant frequency of 5.4 to 48.8%.
DNA was extracted from cyst fluid or pancreatic juice in 5 patients with intraductal papillary mucinous neoplasms. We analyzed 36 cancer related gene mutations by next generation sequencer. Four GNAS and 2 KRAS mutations were detected with variant frequency of 2-29.8 and 6-21% respectively.

Research Products

• [Journal Article] Hepatobiliary and Pancreatic: A splenic artery aneurysm presenting as a calcified pancreatic mass. (2015)
  • Author(s): Kawakubo K, Kawakami H, Kuwatani M, Sakamoto N.
  • Journal Title: J Gastroenterol Hepatol. Volume: 30 Issue: 4 Pages: 655-655
  • DOI: 10.1111/jgh.12848
  • Peer Reviewed
• [Journal Article] Technical tips and troubleshooting of endoscopic biliary drainage for unresectable malignant hilar biliary obstruction. (2015)
  • Author(s): Kawakami H, Itoi T, Kuwatani M, Kawakubo K, Kubota Y, Sakamoto N.
  • Journal Title: J Hepatobiliary Pancreat Sci. Volume: 22 Issue: 4
  • DOI: 10.1002/jhbp.186
  • NAID: 120005740324
  • Peer Reviewed
• [Journal Article] A unique use of a double-pigtail plastic stent: correction of kinking of the common bile duct due to a metal stent. (2015)
  • Author(s): Kuwatani M, Kawakami H, Abe Y, Kawahata S, Kawakubo K, Kubo K, Sakamoto N.
  • Journal Title: Gut Liver. Volume: 9 Issue: 2 Pages: 251-252
  • DOI: 10.5009/gnl14286
  • NAID: 120005600753
  • Peer Reviewed / Open Access
• [Journal Article] Incidental Pancreatic Cyst is a Significant Predictor of Mortality? What Determines Life Expectancy? (2015)
  • Author(s): Kawakubo K, Kawakami H, Kuwatani M, Sakamoto N.
  • Journal Title: Radiology. Volume: 274 Issue: 3 Pages: 939-940
  • DOI: 10.1148/radiol.14141965
  • Peer Reviewed
• [Journal Article] Single-step simultaneous side-by-side placement of a self-expandable metallic stent with a 6-Fr delivery system for unresectable malignant hilar biliary obstruction: a feasibility study. (2015)
  • Author(s): Kawakubo K, Kawakami H, Kuwatani M, Kudo T, Abe Y, Kawahata S, Kubo K, Kubota Y, Sakamoto N.
  • Journal Title: J Hepatobiliary Pancreat Sci. Volume: 22 Issue: 2 Pages: 151-155
  • DOI: 10.1002/jhbp.173
  • NAID: 40020363680
  • Peer Reviewed
• [Journal Article] Risk factors for technical failure of endoscopic double self-expandable metallic stent placement by partial stent-in-stent method. (2015)
  • Author(s): Kawakubo K, Kawakami H, Toyokawa Y, Otani K, Kuwatani M, Abe Y, Kawahata S, Kubo K, Kubota Y, Sakamoto N.
  • Journal Title: J Hepatobiliary Pancreat Sci. Volume: 22 Issue: 1 Pages: 79-85
  • DOI: 10.1002/jhbp.170
  • Peer Reviewed
• [Journal Article] CTNNB1 mutational analysis of solid-pseudopapillary neoplasms of the pancreas using endoscopic ultrasound-guided fine-needle aspiration and next-generation deep sequencing. (2014)
  • Author(s): Kubota Y, Kawakami H, Natsuizaka M, Kawakubo K, Marukawa K, Kudo T, Abe Y, Kubo K, Kuwatani M, Hatanaka Y, Mitsuhashi T, Matsuno Y, Sakamoto N.
  • Journal Title: J Gastroenterol. Volume: in press Issue: 2 Pages: 203-210
  • DOI: 10.1007/s00535-014-0954-y
  • NAID: 120005693897
  • Peer Reviewed
• [Journal Article] High and low negative pressure suction techniques in EUS-guided fine-needle tissue acquisition by using 25-gauge needles: a multicenter, prospective, randomized, controlled trial. (2014)
  • Author(s): Kudo T, Kawakami H, Hayashi T, Yasuda I, Mukai T, Inoue H, Katanuma A, Kawakubo K, Ishiwatari H, Doi S, Yamada R, Maguchi H, Isayama H, Mitsuhashi T, Sakamoto N
  • Journal Title: Gastrointest Endosc. Volume: 80 Issue: 6 Pages: 1030-1037
  • DOI: 10.1016/j.gie.2014.04.012
  • NAID: 120005539217
  • Peer Reviewed
• [Journal Article] Difference from bile duct cancer and relationship between bile duct wall thickness and serum IgG/IgG4 levels in IgG4-related sclerosing cholangitis. (2014)
  • Author(s): Kuwatani M, Kawakami H, Zen Y, Kawakubo K, Kudo T, Abe Y, Kubo K, Sakamoto N.
  • Journal Title: Hepatogastroenterology. Volume: 61 Pages: 1852-1856
  • NAID: 120005672362
  • Peer Reviewed
• [Journal Article] Intravenous and intraperitoneal paclitaxel with S-1 for refractory pancreatic cancer with malignant ascites: an interim analysis. (2014)
  • Author(s): Takahara N, Isayama H, Nakai Y, Sasaki T, Ishigami H, Yamashita H, Yamaguchi H, Hamada T, Uchino R, Mizuno S, Miyabayashi K, Mohri D, Kawakubo K, Kogure H, Yamamoto N, Sasahira N, Hirano K, Ijichi H, Tateishi K, Tada M, Kitayama J, Watanabe T, Koike K.
  • Journal Title: J Gastrointest Cancer. Volume: 45(3) Issue: 3 Pages: 307-11
  • DOI: 10.1007/s12029-014-9603-1
  • Peer Reviewed / Open Access
• [Journal Article] Transmural biliary drainage can be an alternative to transpapillary drainage in patients with an indwelling duodenal stent. (2014)
  • Author(s): Hamada T, Isayama H, Nakai Y, Kogure H, Yamamoto N, Kawakubo K, Takahara N, Uchino R, Mizuno S, Sasaki T, Togawa O, Matsubara S, Ito Y, Hirano K, Tsujino T, Tada M, Koike K.
  • Journal Title: Dig Dis Sci. Volume: 59 Issue: 8 Pages: 1931-8
  • DOI: 10.1007/s10620-014-3062-1
  • Peer Reviewed
• [Journal Article] Slow pull versus suction in endoscopic ultrasound-guided fine-needle aspiration of pancreatic solid masses. (2014)
  • Author(s): Nakai Y, Isayama H, Chang KJ, Yamamoto N, Hamada T, Uchino R, Mizuno S, Miyabayashi K, Yamamoto K, Kawakubo K, Kogure H, Sasaki T, Hirano K, Tanaka M, Tada M, Fukayama M, Koike K.
  • Journal Title: Dig Dis Sci. Volume: 59 Issue: 7 Pages: 1578-85
  • DOI: 10.1007/s10620-013-3019-9
  • Peer Reviewed
• [Journal Article] Safety and utility of single-session endoscopic ultrasonography and endoscopic retrograde cholangiopancreatography for the evaluation of pancreatobiliary diseases. (2014)
  • Author(s): Kawakubo K, Kawakami H, Kuwatani M, Haba S, Kudo T, Abe Y, Kawahata S, Onodera M, Ehira N, Yamato H, Eto K, Sakamoto N.
  • Journal Title: Gut Liver. Volume: 8 Issue: 3 Pages: 329-332
  • DOI: 10.5009/gnl.2014.8.3.329
  • NAID: 120005455894
  • Peer Reviewed / Open Access
• [Journal Article] Multicenter retrospective study of endoscopic ultrasound-guided biliary drainage for malignant biliary obstruction in Japan. (2014)
  • Author(s): Kawakubo K, Isayama H, Kato H, Itoi T, Kawakami H, Hanada K, Ishiwatari H, Yasuda I, Kawamoto H, Itokawa F, Kuwatani M, Iiboshi T, Hayashi T, Doi S, Nakai Y.
  • Journal Title: J Hepatobiliary Pancreat Sci. Volume: 印刷中 Issue: 5 Pages: 328-334
  • DOI: 10.1002/jhbp.27
  • Peer Reviewed
• [Journal Article] Influence of the safety and diagnostic accuracy of preoperative endoscopic ultrasound-guided fine-needle aspiration for resectable pancreatic cancer on clinical performance. (2014)
  • Author(s): Kudo T, Kawakami H, Kuwatani M, Eto K, Kawahata S, Abe Y, Onodera M, Ehira N, Yamato H, Haba S, Kawakubo K, Sakamoto N.
  • Journal Title: World J Gastroenterol. Volume: 20 Issue: 13 Pages: 3620-3627
  • DOI: 10.3748/wjg.v20.i13.3620
  • Peer Reviewed / Open Access
• [Journal Article] Acute pancreatitis caused by Anisakis. (2014)
  • Author(s): Yamato H, Kawakami H, Takagi K, Ogawa K, Hatanaka K, Yamamoto Y, Naruse H, Kawakubo K, Sakamoto N.
  • Journal Title: Gastrointest Endosc. Volume: 79 Issue: 4 Pages: 676-676
  • DOI: 10.1016/j.gie.2013.11.026
  • Peer Reviewed
• [Journal Article] Is intraductal papillary mucinous neoplasm of the pancreas just a precursor or identical with pancreatic ductal adenocarcinoma? (2014)
  • Author(s): Kawakubo K, Kawakami H, Kuwatani M, Sakamoto N.
  • Journal Title: Am J Gastroenterol. Volume: 109 Issue: 3 Pages: 446-447
  • DOI: 10.1038/ajg.2013.468
  • Peer Reviewed
• [Journal Article] Disease-Specific Mortality Among Patients With Intraductal Papillary Mucinous Neoplasm of the Pancreas (2014)
  • Author(s): Kawakubo K, Tada M, Isayama H, Sasahira N, Nakai Y, Takahara N, Uchino R, Hamada T, Miyabayashi K, Yamamoto K, Mizuno S, Mohri D, Kogure H, Sasaki T, Yamamoto N, Hirano K, Ijichi H, Tateishi K, Koike K
  • Journal Title: Clin Gastroenterol Hepatol Volume: 12(3) (2013 Jul 25) Issue: 3 Pages: 486-91
  • DOI: 10.1016/j.cgh.2013.06.032
  • Peer Reviewed
• [Presentation] EUS-guided choledochoduodenostomy with a conventional metallic stent: outcome of a single center experience (2014)
  • Author(s): Kawakubo K, Kawakami, H, Kuwatani, M, Sakamoto, N
  • Organizer: SGI2014
  • Place of Presentation: ソウル（大韓民国）
  • Year and Date: 2014-10-25 – 2014-10-26
• [Presentation] Feasibility of single-step simultaneous side-by-side placement using 6Fr self-expandable metallic stent (2014)
  • Author(s): Kawakubo K, Kawakami H, Kuwatani M, Abe Y, Kawahata S, Kubota Y, Kubo K, Sakamoto N
  • Organizer: DDW2014
  • Place of Presentation: シカゴ（米国）
  • Year and Date: 2014-05-03 – 2014-05-06
• [Presentation] Risk factors for technical failure of endoscopic multiple metallic stent placement by stent-in-stent method for unresectable malignant hilar biliary obstruction (2014)
  • Author(s): Kawakubo K, Kawakami H, Kuwatani M, Abe Y, Kawahata S, Kubota Y, Kubo K and Sakamoto N
  • Organizer: DDW2014
  • Place of Presentation: シカゴ（米国）
  • Year and Date: 2014-05-03 – 2014-05-06
• [Book] 【消化器疾患に対する内視鏡的アプローチの最新情報】 胆道疾患に対するInterventional EUSの現況と展望. (2014)
  • Author(s): 川久保和道, 河上洋, 桑谷将城, 羽場真, 阿部容子, 川畑修平, 久保田良政, 工藤大樹, 久保公利, 坂本直哉.
  • Total Pages: 7
  • Publisher: 映像情報Medical
• [Book] 【患者にやさしいERCPの工夫】 Wire-guided cannulation(WGC)により胆管挿管率の向上とERCP後膵炎の合併の低下は得られるのか? (2014)
  • Author(s): 河上洋, 桑谷将城, 川久保和道, 工藤大樹, 阿部容子, 久保公利, 久保田良政, 坂本直哉.
  • Total Pages: 8
  • Publisher: 消化器内科